Nimagen Molecular Products

BL21(DE3)pLysS Competent E. Coli

Description:

High yields, high efficiency, no heat shock necessary.

Application:

  • Ideal for expressing proteins that are toxic to E.Coli.
  • BL21(DE3)pLysS cells carry the lambda DE3 lysogen. In addition, L21(DE3)pLysS cells contain the pLysS plasmid, which constitutively expresses T7 lysozyme. T7 lysozyme reduces the basal expression of target genes by inhibiting T7 RNA polymerase. This provides tight control of T7 RNA polymerase,which is necessary when the recombinant protein to be expressed is toxic.

Recommended storage condition:

This product should be stored at -80°C. Thaw on ice only before use. Do not re-freeze.

Recommended reaction conditions:

  • Mix DNA with competent cell
  • Let stand on bench for 5 minutes
  • Direct plate

Genotype:

F- ompT gal dcm lon hsdSB(rB- mB-) λ(DE3) pLysS(cmR)

Reference:

Miroux B, Walker JE. J. Mol. Biol. (1996) 260, 289-298.

Competent Cell Type:

Chemically

Transformation Efficiency (cfu/μg):

>1x10^8

Blue-Whiet Screening: 

No

Strain: 

B

Reduces Recombination:

No

Cloning Methylated DNA:

No

Improves Plasmid Quality:

No

Preparing Unmethylated DNA:

Not Suitable

T1 Phage Resistant:

No

RecA Deficient:

No