Nimagen Molecular Products

Dh5-Alpha Competent E. Coli

Description:

DH5a is the most frequently used E.Coli strain for routine cloning applications. In addition to supporting blue/white screening recA1 and endA1 mutations in DH5a increase insert stability and improve the quality of plasmid DNA prepared from minipreps.

Application:

  • Highest transformation efficiency
  • General cloning
  • Blue-white selection
  • Plasmid isolation

Recommended storage condition:

This product should be stored at -80°C. Thaw on ice only before use. Do not re-freeze.

Genotype:

F- 80dlacZ M15 (lacZYA-argF) U169 recA1 endA1hsdR17(rk-, mk+) phoAsupE44 -thi-1 gyrA96 relA1

Reference:

Woodcock, D.M., Crowther, D.M., Doherty, J., Jefferson,S., DeCruz, E., Noyer-Weidner, M., Smith, S.S., Michael, M.Z., and Graham, M.W., Nucl. Acids Res. (1989) 17, 3469-3478

Competent Cell Type:

Chemically

Transformation Efficiency (cfu/μg): 

>3x10^9

Blue-Whiet Screening: 

Yes

Strain:

K12

Reduces Recombination:

Yes

Cloning Methylated DNA:

No

Improves Plasmid Quality:

Yes

Preparing Unmethylated DNA:

Not Suitable

T1 Phage Resistant:

No

RecA Deficient:.

Yes