Competent E.coli

Dh5-Alpha Competent E. coli

DH5a is the most frequently used E.Coli strain for routine cloning applications. In addition to supporting blue/white screening recA1 and endA1 mutations in DH5a increase insert stability and improve the quality of plasmid DNA prepared from minipreps.

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Ordering information

  • Dh5-Alpha Competent E.coli, 20 tubes
    20 x 50 µl (20 tubes)
    SKU: DA-100
  • Dh5-Alpha Competent E.coli, 96-well plate
    96 x 50µl (96-well plate)
    SKU: DA-196

Genotype:

F- 80dlacZ M15 (lacZYA-argF) U169 recA1 endA1hsdR17(rk-, mk+) phoAsupE44 -thi-1 gyrA96 relA1

Competent Cell Type:

Chemically

Transformation Efficiency (cfu/μg): 

>3x10^9

Blue-Whiet Screening: 

Yes

Strain:

K12

Reduces Recombination:

Yes

Cloning Methylated DNA:

No

Improves Plasmid Quality:

Yes

Preparing Unmethylated DNA:

Not Suitable

T1 Phage Resistant:

No

RecA Deficient:.

Yes

This product should be stored at -80°C. Thaw on ice only before use. Do not re-freeze.

  • Highest transformation efficiency
  • General cloning
  • Blue-white selection
  • Plasmid isolation
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