Direct Detection by NGS in one step on raw sample swab material

Generate 10 hotspot Sequencing data direct from RNA in a single tube closed reaction: RNA in, Illumina-ready, indexed library out  



In the current coronavirus pandemic, Real-Time qPCR is worldwide the gold method for detecting SARS-CoV-2 in large scale population screenings. The method is very accurate and sensitive, however only gives a positive or negative results. After successfully developing our RC-PCR based WGS assay, NimaGen is now working on a detection assay as a replacement for qPCR, that has a number of additional features, next to a pos/neg results: Genotyping of 9 hotspot mutations, detecting other winterviruses, like FluA/B, skipping RNA extraction, and increased throughput. This new approach using NGS based detection is secure, and results are accurate and more extensive.

RT-RC-PCR, the next revolution in NGS Library Prep

- High-Throughput library generation, using raw swab material as input in a one-step, closed-tube library prep                  -- Less Handling, Less Risk, Greater Sample Safety
- Easily adapted for Automation
- Combined Reverse Transcription, Amplification and Indexing 

With the NimaGen EasySeq™ RC-PCR Library Preparation method the implementation of an NGS driven viral surveillance strategy could not be simpler or safer. Utilising patented RC-PCR technology, sequence ready libraries are generated from raw samples covering 9 hotspot mutation regions of the SARS-CoV-2 Genome with minimal hands-on time and just a single PCR for Reverse Transcription, Amplification and indexing. Just add buffer from the nasopharyngeal swab and the RT-RC-PCR mastermix provided in the kit and load on to your PCR instrument. Post PCR all samples can be combined for pooled cleanup with the included Ampliclean Magnetic Bead Cleanup Kit and following standard quantification and QC checks your libraries are ready to sequence.


Next Generation Sequencing


NimaGen is currently working on optimization and validation of this new NGS based approach for coronavirus detection. Throughput will be >2000 samples on a MiniSeq rapid run, based on a 10% positive rate. Current detection limit is 10-100 viral copies. If you want to know more about this method, please contact us on info@nimagen.com.


EasySeq™ SARS-CoV-2 WGS Library Prep Kit, 96 rxn

Product Information

RC-PCR, the next revolution in NGS Library Prep


Generate Whole Genome Sequencing data from cDNA, derived from Covid-19 positive tested patients.


Compatible with any low- and mid throughput illumina® NGS sequencer of the latest generations: iSeq®, MiniSeq®, MiSeq®, NextSeq®, in combination with 2x150 bp paired-end read chemistry.

Premium features
Premium features

High performance, great value

The simplicity and security of RC-PCR.
Single pre-PCR reaction setup - Just add cDNA and Mastermix to UDI index plates and go. Post- PCR pool all samples, perform single clean-up reaction, QC, quantify and sequence.
Hands on time 60 minutes, RCPCR and bead clean-up.
Ideal for HTP automation.
Less handling steps, less risk of contamination or sample mix up, greater safety and reassurance.

How can we help to find the right answer for your question. If your question is not listed below, please use the contact form.

What is the maximum Ct value for a positive sample to be able to sequence?

We tested the kit with samples of Ct32 or lower. For samples with Ct <25 we recommend to dilute the cDNA