In the current coronavirus pandemic, Real-Time qPCR is worldwide the gold method for detecting SARS-CoV-2 in large scale population screenings. The method is very accurate and sensitive, however only gives a positive or negative results. After successfully developing our RC-PCR based WGS assay, NimaGen is now working on a detection assay as a replacement for qPCR, that has a number of additional features, next to a pos/neg results: Genotyping of 9 hotspot mutations, detecting other winterviruses, like FluA/B, skipping RNA extraction, and increased throughput. This new approach using NGS based detection is secure, and results are accurate and more extensive.
- High-Throughput library generation, using raw swab material as input in a one-step, closed-tube library prep -- Less Handling, Less Risk, Greater Sample Safety
- Easily adapted for Automation
- Combined Reverse Transcription, Amplification and Indexing
With the NimaGen EasySeq™ RC-PCR Library Preparation method the implementation of an NGS driven viral surveillance strategy could not be simpler or safer. Utilising patented RC-PCR technology, sequence ready libraries are generated from raw samples covering 9 hotspot mutation regions of the SARS-CoV-2 Genome with minimal hands-on time and just a single PCR for Reverse Transcription, Amplification and indexing. Just add buffer from the nasopharyngeal swab and the RT-RC-PCR mastermix provided in the kit and load on to your PCR instrument. Post PCR all samples can be combined for pooled cleanup with the included Ampliclean Magnetic Bead Cleanup Kit and following standard quantification and QC checks your libraries are ready to sequence.
NimaGen is currently working on optimization and validation of this new NGS based approach for coronavirus detection. Throughput will be >2000 samples on a MiniSeq rapid run, based on a 10% positive rate. Current detection limit is 10-100 viral copies. If you want to know more about this method, please contact us on firstname.lastname@example.org.
EasySeq™ SARS-CoV-2 WGS Library Prep Kit, 96 rxn
RC-PCR, the next revolution in NGS Library Prep
Generate Whole Genome Sequencing data from cDNA, derived from Covid-19 positive tested patients.
Compatible with any low- and mid throughput illumina® NGS sequencer of the latest generations: iSeq®, MiniSeq®, MiSeq®, NextSeq®, in combination with 2x150 bp paired-end read chemistry.
High performance, great value
How can we help to find the right answer for your question. If your question is not listed below, please use the contact form.
What is the maximum Ct value for a positive sample to be able to sequence?
We tested the kit with samples of Ct32 or lower. For samples with Ct <25 we recommend to dilute the cDNA