Application

GreenoType®  
Customized Solutions for Agri Science.

GreenoType accelerates breeding programmes and Quality Assurance, while being very cost-effective compared to other technologies like two-step PCR library prep, KASP, TaqMan, Fluidigm, ddPCR.

GreenoType Plant Genotyping
Greenotype RC-PCR

Greenotype RC-PCR

High-Throughput screening by the most robust, cost effective and simple method available, NimaGen offers the patented RC-PCR technology for SNP Genotyping for up to 100 SNPs per assay*. Genotyping by NGS will never be easier: Just run a single PCR followed immediatly by pooling, single-tube cleanup and sequencing on an illumina NGS system.

GreenoType RC-PCR is also the method of choice for contamination detection in seed production, with a sensitivity of 0.33% (1 in 300 individual seeds in one pool).

Just provide us with your required targets**, including 100 bp up- and downstream, 5 reference DNA samples of your crop of interest, and NimaGen will design and wet validate your GreenoType Assay.

For more information, please contact us by fliing in the contact form below.

*300 plex soon available

**include 30% more targets to allow us to drop bad performers

GreenoType MIP capture

GreenoType MIP capture

With Molecular Inversion Probe capture, NimaGen offers a Medium to High Multiplex Genotyping-by-NGS solution for up to 5000 SNPs per sample. The three step protocol consist of a Capture/Ligation step, followed by a quick Exonuclease treatment and a final PCR. The MIP technology is robust and cost-effective.

Just provide us with your required SNP targets* in a bed file, including your reference genome and VCF file, 5 reference DNA samples of your crop of interest, and NimaGen will design and wet validate your GreenoType Assay.

For more information, please contact us by fliing in the contact form below.

*include 30% more targets to allow us to drop bad performers

Custom made

Custom kits

NimaGen offers complete solutions for your custom NGS kits.

Please provide us with some info regarding your needs, and we will contact you as soon as possible.

More Information