Sanger sequencing is a method of DNA sequencing, based on the selective incorporation of chain-terminating dideoxynucleotides by DNA polymerase during in vitro DNA replication, developed by Frederick Sanger and colleagues in 1977. Since the introduction of massive parallel sequencing (NGS), the Sanger method remains in wide use, for smaller-scale projects, validation of NGS results and for obtaining especially long contiguous DNA sequence reads.
Fluorescent DNA Fragment Analysis
Fluorescent DNA fragment analysis is one of the most useful methods in molecular biology. The method measures the relative size of DNA fragments with a very high resolution and reproducibility, by capillary electrophoresis (CE) of fluorescent labelled DNA fragments on an automated DNA CE Genetic Analyzer, using internal fluorescent size standards.