Application

Sanger
Sequencing

Introduction

The gold standard

Sanger sequencing is a method of DNA sequencing, based on the selective incorporation of chain-terminating dideoxynucleotides by DNA polymerase during in vitro DNA replication, developed by Frederick Sanger and colleagues in 1977. Since the introduction of massive parallel sequencing (NGS), the Sanger method remains in wide use, for smaller-scale projects, validation of NGS results and for obtaining especially long contiguous DNA sequence reads.

Fluorescent DNA fragment analysis is one of the most useful methods in molecular biology. The method measures the relative size of DNA fragments with a very high resolution and reproducibility, by capillary electrophoresis (CE) of fluorescent labelled DNA fragments on an automated DNA CE Genetic Analyzer, using internal fluorescent size standards.

Portfolio

NimaGen offers a complete portfolio of high-quality and cost-effective reagents for Applied Biosystems capillary electrophoresis based Genetic Analyzers, like the 310, 3100 series, 3130 series, 3500 series, 3730 series and SeqStudio.

Sanger Sequencing

Products