31-08-2018

NimaGen's smMIP application improves the potato breeding strategy of HZPC

Application note

Introduction

Currently, Marker Assisted Breeding (MAB) is often supported by TaqMan® or KASP genotyping assays to identify single nucleotide polymorphisms (SNPs). These methods are cost-effective and can be applied in a high throughput setting.

Because of the strength of an Next Generation Sequencing (NGS) based method and user-friendliness of this platform, the EasySeq NGS Targeted Capture kit is applied, using tailor-made Molecular Inversion Probes (smMIP) derived from the potato genome.

Initially this technology is applied in research, but the perspective is that this technology in the future will be used also in the standard MAB operations. This allows for high throughput screening, up to multiple thousands of samples per year. An improved lab efficiency of the workflow and the throughput capabilities fit the needs of the company, making EasySeq NGS Targeted Capture kits a cost effective workflow.

Conclusion

The implementation of the smMIP technology of the EasySeq NGS Targeted Capture kits will provide HZPC with microhaplotypes for better and faster understanding of complex traits and EasySeq NGS Targeted Capture kit applies a cost effective method and could performed in a high throughput fashion. Furthermore it provides HZPC with a new tool for selection of better potato varieties in the future.

Read the full application note HERE.

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