BDR kits

GenVinSet HLA B27 Detection Kit

SKU:

Assay for detection of all HLA B27 group alleles

  • Detection of the HLA B27 alleles
  • Real-Time PCR
  • Easy interpretation and clear results
  • No need for conventional DNA extraction
  • No electrophoresis
  • CE/IVD certified kit

   

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The major histocompatibility complex (MHC) is the genetic region that contains the most polymorphic loci of the genome involved in the mechanism of antigen presentation and, as such, defines the general immunological response.

Within the MHC, the allelic family HLA B27 is part of the HLA-B locus and presents a frequency between 3 and 8% of the caucasian population. Despite this frequency the interest in this allelic family relies in its relation with different rheumatic diseases called spondyloarthropathies, from which we can highlight ankylosing spondylitis (AS).

Around 90% of the patients with AS are HLA B27 positive. Other related autoimmune diseases are Juvenile rheumatoid arthritis (80% of patients), and the Reiter syndrome or reactive arthritis (50-80%).

Detection method

The detection method used by Genvinset is based in a primer specific PCR, which anneal to all the alleles of the HLA-B27 family (*) monitored with Taqman® probes. At the same time the method amplifies and detects a control gene (ß-globin) to verify the assay’s result. This technique provides high resolution because each pair of primers recognizes 2 DNA areas situated in cis, resulting in high sensitivity, specificity and reproducibility.

HLA B27 Detection workflow

Kit contents

Reference GVSB2702-48 (48 tests)
• GVSB27PMv2: 2 vials x 83 μL Primer Mix (PM)
• GVSB27LS: 3 x 1.7 mL Lysis Solution (LS)
• GVSB27NC: 1 x 100 μL of Negative Control (NC

Kit storage

All of the kit’s reagents should be stored from -18ºC to -30ºC and they are stable at this temperature until its expiration date, as indicated in the bottle. Do not perform more than 3 freezing/thawing cycles to the Primer Mix vials (GVSB27PMv2) as this could reduce the assays sensitivity and change results. After receiving the kit, and once the lysis solution is unfrozen, store at room temperature (15-30ºC). Due to the reagent’s photo sensitivity nature, avoid continuous exposure to light.

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