BRCA12PCR

EasySeq PCR plates for BRCA1 and BRCA2 screening

Use our EasySeq PCR plates for fast, straightfoward and robust mutation screening of the BRCA1 and BRCA2 genes.

BRCA12PCRTXT

 

  • Based on the Gold Standard Sanger Sequencing
  • Sample in -> result out: no need for batching up samples
  • Thorough coverage: 100% gene resequencing
  • Minimal hands-on time
  • Compatible with:
    - Standard DyeTerminator sequencing workflows
    - BrilliantDye® Terminator Cycle Sequencing Kit
    - BigDye® Direct
  • Primers tailed with M13 sequences: Universal Cycle Sequencing

 

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Ordering information

  • EasySeq PCR plates, 5 plates
    For BRCA1/2 Mutation Detection
    SKU: 350596
  • EasySeq PCR plates, 25 plates
    For BRCA1/2 Mutation Detection
    SKU: 352596
  • EasySeq single amplicon primer pair
    50 rxn for BRCA1/2 mutation detection, in tube format
    SKU: 355001
  • EasySeq complete set of single amplicon primer pairs
    80 primer pairs of 10 rxns each for BRCA1/2 mutation detection, 96-well plate (Dried Down)
    SKU: 355010
  • EasySeq complete set of single amplicon primer pairs
    80 primer pairs of 50 rxns each for BRCA1/2 mutation detection, in 96-well plate format
    SKU: 355050

The unmatched fast, simple, robust and streamlined workflow for detecting mutations in the BRCA1 and BRCA2 genes. EasySeq™ PCR plates for BRCA1 and BRCA2 sequencing are offered in prespotted semi-skirted PCR plates. The plates contain primer pairs to generate 80 PCR products. These primer pairs are designed and optimized so that the gained PCR product covers 100% of the BRCA1 and BRCA2 genes. Also included are sections of approximately 50 base pairs up- and downstream of each exon.

For easy conversion and maximum flexibility of your worklow the plates contain 15 multiplex non-template control wells to prove the specificity of the results.

 Additional information

All primer pairs used in the EasySeq™ PCR plates are also available in separate tubes, enough for 50 rxns, to enable re-analysis of specific amplicons in case of failure, and to screen DNA samples for specific exons or mutations. The primer pairs are dried down in screw-capped tubes. In the description you will find between brackets the original position of this primer pair in the EasySeqPCR plate.

AMBeR sequencing primers are modified primers, which are used to improve resolution and mobility of the first 50 bases in your sequencing chromatograms. They can be ordered as ready to use primers in combination with the EasySeq PCR plates or as a custom primer.

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