Easyseq BOX

EasySeq NGS Targeted Capture Kit for Hereditary Breast and Ovarian Cancer (HBOC)

Now Available!

The EasySeq NGS Targeted Capture Kits provide a complete workflow for routine Next Generation DNA sequencing. Using sequence capturing with single molecule Molecular Inversion Probes, followed by a barcoding amplification step, the system enables sequencing of the targeted regions in a very simple, fast and robust workflow, resulting in coverage with double probe tiling and independent reading of both strands. The kits contain all reagents for a straightforward, one-step capturing and subsequently sample tagging for NGS sequencing. The resulting libraries are compatible with Illumina® sequencing platforms.

101Box

 

For data analysis, NimaGen is partnered with JSI Medical Systems logo-JSIto provide a powerful all-in-one solution for robust high-throughput variant detection and interpretation the NGS data.

Interested? Choose one of the options below to view pricing

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Ordering information

  • EasySeq™ NGS Targeted Capture Kit HBOC 10 genes, 24 Samples
    Includes BRCA1, BRCA2, PALB2, CHEK2, CDH1, RAD51C, RAD51D, TP53, BRIP1, ATM
    SKU: ESI-HBOC10-024
  • EasySeq™ NGS Targeted Capture Kit HBOC 10 genes, 96 Samples
    Includes BRCA1, BRCA2, PALB2, CHEK2, CDH1, RAD51C, RAD51D, TP53, BRIP1, ATM
    SKU: ESI-HBOC10-096
  • EasySeq™ NGS Targeted Capture Kit HBOC 7 genes, 24 Samples
    Includes BRCA1, BRCA2, PALB2, CHEK2, RAD51C, RAD51D, BRIP1
    SKU: ESI-HBOC7-024
  • EasySeq™ NGS Targeted Capture Kit HBOC 7 genes, 96 Samples
    Includes BRCA1, BRCA2, PALB2, CHEK2, RAD51C, RAD51D, BRIP1
    SKU: ESI-HBOC7-096
  • EasySeq™ NGS Targeted Capture Kit HBOC 5 genes, 24 Samples
    Includes BRCA1, BRCA2, PALB2, CHEK2, ATM
    SKU: ESI-HBOC5-024
  • EasySeq™ NGS Targeted Capture Kit HBOC 5 genes, 96 Samples
    Includes BRCA1, BRCA2, PALB2, CHEK2, ATM
    SKU: ESI-HBOC5-096
  • Coding regions 100% covered including intron-exon boundaries in one single tube assay

  • Based on Molecular Inversion Probe capturing with single molecule tags (smMIPs)

  • Brand new design, containing >1300 smMIPs probes with novel backbone

  • Simple and robust workflow, extremely low hands-on time: < 1 hour

  • Intrinsic refSNPs for sample ID and quality control

  • Single Click Analysis with JSI SeqNext Software

  • Compatible with all Illumina® Sequencing Platforms

 

Compared to other commercial available sequencing enrichment systems, EasySeqTM NGS kits deliver high quality data with complete and optimized coverage for all exons in both genes without the need of fragmenting the DNA. The nucleotides of the regions are covered by 2 independent smMIP probes for highly trustable data.

In addition, the smMIP pool, provided in this kit, contains genome-wide SNP-MIPs: >45 probes are targeting a set of highly variable Single Nucleotide positions, divided over the Human Genome, enabling sample tracking and heritance checking.


The robust workflow consists of a limited number of hands-on steps, is compatible with standard 96/384 well formats and amendable for automation for high-throughput labs. For downstream data analysis, NimaGen recommends SeqNext from JSI medical systems. Analysis settings templates for the EasySeq generated sequence data will be available thru JSI (www.JSI-medisys.de).A further, unique feature of smMIP enrichment is the extreme high rate of quantitative reproducibility in coverage. Due to the single molecule tag of 8 degenerative bases, the generated data can distinguish original, unique captures from reads caused by amplification bias. This enables researchers to minimize read errors, and discovery of very low mutation rates with the generated sequencing data, even on DNA derived from FFPE samples.

Looking for a selection of a panel with one ore more genes from HBOC-10 panel? Or additional genes? Please contact us to inquire about the many possibilities!

 

HBOC-5

HBOC-7

HBOC-10

BRCA1

BRCA1

BRCA1

BRCA2

BRCA2

BRCA2

PALB2

PALB2

PALB2

CHEK2

CHEK2

CHEK2

 ATM

RAD51C

RAD51C

 

RAD51D

RAD51D

 

BRIP1 

CDH1

 

 

TP53

 

 

ATM

 

 

BRIP1

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