High Quality hot-start DNA polymerase
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HOTaq DNA Polymerase is hot-start Taq DNA Polymerase, which is a modified form of Taq DNA Polymerase. HOTaq DNA Polymerase is provided in an inactive state and has minimum enzymatic activity at ambient temperatures. This prevents the formation of misprimed products during reaction setup and the first denaturation step, leading to high PCR specificity. It is suitable for diagnostic reaction without miner band. The enzyme is a highly processive 5'-> 3' DNA polymerase that lacks 3'-> 5' exonuclease activity. Each lot of HOTaq DNA polymerase is tested for PCR amplification.
The product is a chemically modified form of the Taq enzyme. It will be inactive at room temperature, and become active after 10 minutes at 94ºC.
An E. coli strain that carries the Taq DNA Polymerase gene from Thermus aquaticus.(same as Taq DNA Polymerase)
94°C, 10min. -> (94°C, 10sec. -> 55°C, 30sec. ->72°C, 30sec.) for 25 cycles.
One unit is defined as the amount of enzyme that will incorporate 10 nmol of dNTP into acid-insoluble material in 30 minutes at 75°C.
Here is the result of comparing MCLAB's HoTaq with some other leading brands.