Hotsybr mastermix

2x HotSybr Real-Time PCR Kit-MCLab

High performance Sybr® Green Real-Time PCR master mix

  • Similar features to ABI's Power Sybr® Green PCR Master Mix
  • High sensitivity: Less than 10 copies detected per well
  • Wide dynamic range: More than 5 orders of magnitude
  • Consistent batch-to-batch reproducibility: ±1.0 fluorescence threshold

 

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Ordering information

MCLabs' 2X HotSybr Real-Time PCR Kit is similar to ABI's Power Sybr® Green PCR Master Mix. It delivers highly sensitive nucleic acid quantitation. As few as 1-10 copies of a target sequence can be detected. The design of the master mix enables reliable DNA amplification results and lot to lot variation in assay performance is minimized.

Fast sample to result

 mclab_pcr_image002.gif

  • Normally the total reaction time is 4350 sec: 95°C, 10min => (95°C, 15sec => 60°60sec) x 50
  • For 2X HotSybr PCR Reaction Mix, the total reaction time is reduced to 2350 sec: 95°C, 10min => (95°C, 5sec => 60°, 30sec) x 50

Recommended Reaction Conditions

95ºC, 10 minutes > (95ºC, 5 seconds > 60ºC, 30 seconds) for 50 cycles > melting curve.

Recommended storage condition

-20oC

  • Dye based qPCR: including DNA quantification
  • 2-step RT-PCR
  • SNP analysis 

To achieve the best performance, appropriate software, such as ABI Primer Express™, should be used to design primers with 50ºC~65ºC melting temperature. Further guidelines are:

  • Amplicon size should be small, <150bp
  • Avoid secondary structures in primers
  • Avoid more than 3 consecutive Gs in primers
  • Primers should not have complementary 3'-ends
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