Product

Catalyst™ 48 Slotted Magnet Plate

SKU: A000530
Catalyst 48
Product Information

48 well slotted magnet plate for Nest Scientific plates

Alpaqua

The Catalyst 48 slotted ring magnet plate is designed to work with the 48 deep-well round bottom plate by Nest Scientific. Each well of that plate holds up to 3.5 ml, perfect for protocols that require that little bit of extra volume. The 48 well format fills the gap between 96-deep well plates, which top out at 2.2 ml, and the next step up, being a 24 well plate at 8-10 ml.

Compatibility

The deepwell plates used with the Catalyst 48 Magnet Plate are made by Nest Scientific. SKU: 504102

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The combination of the slotted magnets and the round-bottom wells work together extremely well; the beads settle rapidly and form two clean arches at the well bottom, leaving an opening for pipette tips to slide down the well wall towards the bottom without ever touching the beads. At 11 mm diameter, the wells are big enough so one can actually see what the pipet tip is doing inside the well, making this plate very easy to use.

The magnets can be rotated to work by row or by column.

Exceptionally easy to use manually
Round wells for efficient orbital shaking
Faster bead pull-down compared to 96-well format
All-metal construction allows for on-magnet 37º incubation or cooling
Product news

Latest news

News | 30.03.21

Press Release: NimaGen- Expanding Throughput for SARS-CoV-2 Whole Genome Sequencing

Nijmegen, The Netherlands, March 29, 2021   Following the release and success of their EasySeq™ SARS-CoV-2 WGS kit in 2020, NimaGen BV announce the further expansion of their kit range to facilitate higher throughput application of SARS-CoV-2 Whole Genome Sequencing (WGS) with the launch of a 384-sample version kit for Illumina sequencing and the addition of an expanded selection of accompanying Unique Dual Indexes (UDIs) now increased to 1536 UDIs. Rapid and Simplified Library Preparation for viral surveillanceThe recent emergence and spread of new nCoV-2 variants have clearly demonstrated the importance and need for rapid and high throughput screening for SARS-CoV-2 for viral surveillance. Utilising Reverse Complement PCR (RC-PCR), WGS library preparation with the EasySeq™ SARS-CoV-2 kit could not be simpler- with just a single PCR reaction for both Amplicon generation and indexing.Assay set-up is extremely easy and ideally suited for automation, with just 2 dispensing steps of sample and Mastermix to pre-spotted index plates before loading in the Thermocycler. Following the single PCR all samples can immediately be pooled for a single-tube magnetic bead clean-up step and once standard quality control checks have been performed the library is ready for sequencing.This drastically simplified protocol can reduce hands on time for library prep by up to 80% compared with other methods currently being used for the WGS of SARS-CoV-2. Dr Josh Quick from the Institute of Microbiology & Infection, University of Birmingham, UK and who was integrally involved in the creation of the ARTIC Network SARS-CoV-2 sequencing protocol has commented:"Multiplex RC-PCR is one of the solutions emerging from the pandemic that has excited me the most. The approach combines the robustness of amplicon sequencing with the scalability of PCR barcoding in a single PCR step, something I have puzzled over for years. In our testing the sensitivity was comparable to other amplicon based methods but with a greatly streamlined Illumina library preparation workflow and I am optimistic that there is even more performance to come with this approach in future." Simpler and more rapid library prep does allow for more samples to be prepared each day, improving public health authority’s ability to monitor the epidemic and the emergence, and spread of new variants. Sequence every positive COVID-19 Sample?“The addition of a 384 well version of the kit to the range will certainly help our high-throughput customers streamline their library preparation even further” says Joop Theelen, CSO and co-founder of NimaGen “Also, with up to 1536 accompanying UDIs now available, more than 3000 SARS-CoV-2 whole genomes could be sequenced on one Illumina NovaSeq SP dual lane flowcell – bringing the possibility to sequence every positive COVID-19 sample closer than ever before.” Future DevelopmentsNimaGen are also rapidly developing a “one-step” version of the kit that works direct on RNA samples, thus avoiding the need for a separate cDNA synthesis reaction before WGS library preparation.By adapting the RC-PCR method cDNA synthesis, amplification and indexing can be carried out in a single closed tube PCR reaction, further streamlining the complete workflow, and allowing for increased throughput.“The need for a separate cDNA synthesis step is currently a potential major workflow bottleneck for our customers and as with any additional handling step it also adds to the risks around assay and sample security” Walter van der Vliet, NGS Application & Development Scientist at NimaGen says “If we can incorporate the generation of cDNA into the RC-PCR reaction then our customers can go direct from extracted RNA to indexed amplicons in just one PCR step, making the whole workflow faster and more secure and ultimately allowing them to process more samples each day” Early development data looks positive and NimaGen expects to have the product commercially available in spring 2021.Further details available at www.nimagen.com/covid19