Product

dGTP BrilliantDye™ Terminator (v1.1) Cycle Sequencing Kit (100 rxn)

SKU: BRDG1-100
Variants
BRDG1-100
Variants
Product Information

dGTP BrilliantDye™ Terminator (v1.1) Cycle Sequencing Kit for Sequencing G- and GT-rich Templates

Format

dGTP BrilliantDye™ Cycle Sequencing Kits (v1.1 and v3.1) are designed and optimized for challenging sequence templates, by replacing the dITP with dGTP.

 

dGTP BrilliantDye™ Terminator (v1.1) Cycle Sequencing Kit provides the reagents (BrilliantDye Terminator, 5x sequencing buffer, DNA polymerase, pGEM control, M13 control primer) required for Sanger Sequencing of G- en GT-rich sequence templates, in a ready for use pre-mixed format.

 

dGTP BrilliantDye™ Terminator (v1.1) Cycle Sequencing Kit is conveniently offered in low to medium-throughput packaging, for 100 and 1,000 reactions respectively.

Compatibility

dGTP BrilliantDye™ Terminator (v1.1) Cycle Sequencing Kits are compatible with 310, 3100, 3130, 3500, 3730 Series Genetic Analyzers, and SeqStudio™.


dGTP BrilliantDye™ Terminator (v1.1) can be mixed with BrilliantDye™ (v1.1) for optimal results.

 

SeqStudio is a trademark of Applied Biosystems, LLC.

Premium features
FAQ
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Premium features

dGTP BrilliantDye™ Terminator (v1.1) Cycle Sequencing Kits offer robust, highly flexible chemistry, with uniform peak heights and optimized saignal balance. Replacement of dITP with dGTP enables to extend through those difficult-to-sequence regions with confidence, avoiding early signal loss in samples.

dGTP BrilliantDye™ Terminator v1.1 Cycle Sequencing Kit is designed for specialty applications that require optimal basecalling adjacent to the primer.

 

dGTP BrilliantDye™ Terminator v3.1 Cycle Sequencing Kit is the method of choice for longest reads.

Based on the proven Sanger Chain Termination method
No need for changes in protocol, setting or calibration
Available in chemistry versions dGTP v1.1 and dGTP v3.1
Compatible with all Genetic Analyzers
FAQ

How can we help to find the right answer for your question. If your question is not listed below, please use the contact form.

What can I do to sequence through a hairpin or secondairy structure?

- Use the BrilliantDye dGTP kit for the sequencing reaction. For optimal results it can be mixed with the default BrilliantDye kits to increase read lenghts.

- Add DMSO to a final 5% (v/v) concentration to the sequencing reaction.

- Incubate the reaction at 96C for 10 minutes before cycle sequencing.

How can I troubleshoot an issue with Sanger Sequencing?

A control primer (M13) and template (pGEM) are provided with each BrilliantDye kit. These can be run following the BrilliantDye protocol and can help you determine whether failed reactions are caused by poor template quality or sequencing reaction failure.

What is the difference between the different versions of BrilliantDye?

The BrilliantDye Terminator v1.1 Cycle sequencing kit is designed for specialty applications that require optimal basecalling adjacent to the primer.

The BrilliantDye Terminator v3.1. Cycle sequencing kit is the method of choice for longest reads.

For sequencing through G- and GT-rich templates, NimaGen offers the dGTP BrilliantDye kits, optimized for challenging sequence templates.

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