Product

IDseek® Mitochondrial DNA HVR Sequencing by MPS Sample Kit

SKU: IDS-HVR96S
Variants
Required products
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IDseek® Mitochondrial DNA HVR Sequencing by MPS Sample Kit
Variants
Required products
Automatically added to cart
Product Information

IDseek® Mitochondrial DNA HVR Sequencing by MPS Kit

Intended use

Reverse Complement (RC-PCR) based MPS library prep solution for analysis of hypervariable regions (mutation hotspots) in very low quantities of (highly degraded) nuclear mtDNA in complex samples that do not provide a full STR. For laboratories in those countries where full genome sequencing in forensics is restricted by guidelines or regulations. 

 

RC-PCR provides multiplex targeted amplification, sequencing indexing and adaptor addition occuring simultaneously in a single closed tube reaction. 

Compatibility

All amplicons are designed to meet the shortest possible fragment lengths and are compatible with illumina® MiSeq™ systems, with 2x 10 bp Unique Dual Index reads.

 

IDseek® Kits have been tested with Tannic Acid and Humic Acid. RC-PCR technology allows the reliable analysis of highly degraded samples frequently found in forensic casework.

Kit Components

IDseek® Reagents and Consumables

Sequence-ready libraries

IDseek® comprises a complete solution of reagents and consumables required for generating sequence-ready MPS libraries for Forensic applications: from indexing to amplification, magnetic library clean-up and size selection.

IDseek® components

IDseek® reagents and consumables required for 96 reactions:

- RC-PCR Probe Mix
- RC-PCR HiFi 2x Hotstart PCR Mastermix
- 2x Dehydrated Pre-spotted Unique Index Primer Plates
- AmpliClean™ Cleanup, Magnetic Beads


A magnet compatible with 1.5 mL/2.0 mL centrifuge tubes is recommended for use with the AmpliClean™ Magnetic Bead Cleanup.

idseek logo image
News | 13.09.23

NimaGen x NicheVision Partnership Announcement 

NimaGen and NicheVision are partnering to deliver a complete wet-to-dry lab solution for forensic DNA analysis!     This partnership aims to provide forensic DNA laboratories with a combined cutting-edge DNA sequencing and data analysis workflow for human identification, that helps DNA analysts resolve complex DNA mixtures through a fully continuous approach.   The partnership is a distribution and development agreement that grants NicheVision exclusive rights to distribute NimaGen products for forensic DNA analysis in North, Central and South America. The companies will collaborate to commercialize NimaGen’s Reverse Complement PCR powered IDseek® Massively Parallel Sequencing (MPS) workflow in combination with NicheVision MixtureAce™ expert forensic DNA analysis software; as well as NimaGen brand products (NimaPOP™, NimaPrime™) for Genetic Analyzer based Fragment Analysis.  Come see us at ISHI 2023 on Sept 18-21st in Denver, CO! NimaGen will be at Booth #313 and NicheVision will be at Booth #312. Come learn all about the next revolution in Forensic MPS!
News | 16.08.23

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