EasySeq™ 16S rRNA V3V4 Bacterial ID Sequencing Kit

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EasySeq™ 16S rRNA V1-6 and V9 Bacterial ID Sequencing Kit, 96 rxn
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Product Information

16S rRNA V3V4 for maximum discriminatory power with complex samples  

16S rRNA V3V4

All bacteria and archaea contain a ribosomal subunit known as 16S that is around 1,500 nucleotides long and contains nine hypervariable regions (V1-V9) spread out among highly conserved regions that are genus- or species specific. Sequencing of the 16S ribosomal RNA (rRNA) gene, an established genetic marker, is the gold standard for the analysis of bacterial samples for identification and classification of both pure cultures and mixed sample analysis in complex microbial communities, such as soil samples, oral cavity samples, or human gut microbiomes.


The main reason for using the V3 and V4 regions in majority of the cases is that these regions contain the maximum nucleotide heterogeneity and displays the maximum discriminatory power needed for complex samples, to evaluate bacterial diversity and detect the abundance of microbes in various environments.


Kit and Platform Compatibility

Please note: EasySeq™ 16S rRNA V3V4 Bacterial ID NGS Library Prep Kit is designed to sequence the hypervariable regions V3 and V4 in a singleplex reaction, using a single amplicon that is aimed at deconvoluting complex bacterial communities. This requires one Unique Dual Index Plate for associated use.


Compatible with Illumina® MiSeq and NextSeq 1000/2000 (2x250bp) runs, both suitable for 16S metagenomic sequencing.


Are you interested in running an evaluation of the EasySeq™ 18S rRNA and Cyp51A kits? Please send us your request by selecting the 'Inquiry' button.

Premium features
Premium features

This kit generates sequence-ready libraries from (c)DNA, using an efficient, single reaction,  Reverse Complement PCR workflow, combining targeted amplification and indexing in a single tube. Less handling, minimizing the risk of sample contamination.

Key Features

Targeted Sequencing Library Prep by Reverse Complement, assay developed to analyze the 16S rRNA for bacteria identification PCR
Combined target amplification and Indexing with RC-PCR
Less Handling, Less Risk, Greater Sample Safety
Easy to automate
News | 08.05.23

12th Haploid Markers Conference: Come Meet NimaGen's Pieter van Oers and learn all about IDseek®: The Next Revolution in Forensic MPS!

Following our attendance at ESWG ISFG 2023, NimaGen will have our representative Pieter van Oers present at the 12th Haploid Markers Conference in Budapest, Hungary from May 17th-20th, 2023. Come learn all about IDseek®, the next Revolution in Forensic MPS powered by RC-PCR technology!   Our NimaGen representative, Pieter van Oers, look forward to meeting you!   Interested? Learn more at or contact us directly at:
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Advena (Kazakhstan, Uzbekistan, Kyrgyzstan, Armenia): Welcome to the NimaGen Distributor Network!

We are happy to share that we have set up a new collaboration with Advena LLP as of April 1st, 2023. Advena are now the exclusive representatives for the NimaGen portfolio in Kazakhstan, Uzbekistan, Kyrgyzstan and Armenia. The company is headquartered in Astana, Kazakhstan, and their team has a long history in distribution and Life Science. We are excited to be able to support our customers in these countries through the collaboration with Advena.  
Product Information

16S rRNA Library Prep by Reverse Complement PCR

Bacterial Content Determination
Platform Compatibility

This kit is used with DNA extracted from pure culturesand also allow direct amplification of DNA from clinical materials. Libraries are compatible with Illumina®.