EasySeq™ NGS Library Prep by Reverse Complement PCR

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RC-PCR

Contamination Concerns?

Conventional Amplicon based methods for NGS target enrichment involve two PCR steps – one to amplify the regions of interest followed by a second PCR reaction to add adapter sequences for NGS. While these methods are well established there is always a high risk of cross contamination or carry-over from first to second rounds of amplification.

Legal Notice:

RC-PCR is IP protected.

US Patent / WO Patent / EP Patent

Exclusive license granted to NimaGen B.V., Nijmegen, The Netherlands

by SALISBURY NHS FOUNDATION TRUST (SALISBURY, WILTSHIRE, GB)

It is strictly prohibited to use, produce, distribute, import, or sell RC-PCR based methods or kits without the patent owner's written consent.

View products

How it Works

Handling

Easy Handling

Just one Pre-PCR reaction setup, quick, easy and highly suited for automation

Generation

Product Generation is More Target Specific

Traditional PCR reactions have high concentrations of primer at the beginning of the reaction when the template availability is low.

In a RC-PCR the target specific primer is synthesized during the reaction and consequently more aligned with the target availability reducing the chance for dimerization and off target binding.

Multiplex Capabilities

Predesigned and custom panels available targeting up to 100 amplicons in a single closed tube reaction.

Solutions available and in development

Human Sample tracking and ID kit for WGS/WES QC
Forensic Identification Kits
Crop Genotyping assays (GreenoType)
GMO detection kit
Meat detection kit (including speciate)
Pharmacogenetics (PGX)
Circulating Tumour DNA kit
Detection of aneuploidies

Products

EasySeq™ NGS Library Prep by Reverse Complement PCR

Contamination Concerns?

How it Works

Easy Handling

Product Generation is More Target Specific

EasySeq NGS RC-PCR Products

EasySeq™ 18S and Cyp51A rRNA Fungal ID Kit, 96 rxn

EasySeq™ PALB2 Sequencing Kit, 96 rxn

EasySeq™ CFTR HotSpot Sequencing Kit, 96 rxn

EasySeq™ CFTR Sequencing Kit, 96 rxn

EasySeq™ BRCA1/2 + CHEK2 HS Sequencing Kit, 96 rxn

IDseek® mYSTR Y-Chromosomal 27 Short Tandem Repeat Kit, 96 rxn

IDseek® OmniSTR Global 30-plex Short Tandem Repeat Kit, 96 rxn

IDseek® Mitochondrial HVR Sequencing by NGS Kit, 96 rxn

IDseek® Mitochondrial Full Genome Sequencing by NGS Kit, 96 rxn

IDseek® SNP-ID 85-plex Kit, 96 rxn

EasySeq™ TP53 Sequencing Kit, 96 rxn

AmpliClean™ Cleanup Kit, Magnetic Beads (5 mL)

AmpliClean™ Cleanup Kit, Magnetic Beads (50 mL)

AmpliClean™ Cleanup Kit, Magnetic Beads (500 mL)

EasySeq™ SARS-CoV-2 WGS Library Prep Kit, 96 rxn

EasySeq™ SARS-CoV-2 WGS Library Prep Kit, 384 rxn

EasySeq™ Human Exome Sample Tracking Kit for NGS, 96 rxn

EasySeq™ 16S rRNA Bacterial ID Kit, 96 rxn

Custom kits