EasySeq™ NGS Library Prep by Reverse Complement PCR


NGS Library Prep Contamination Concerns?

Conventional amplicon-based methods for NGS Library Prep target enrichment involve two PCR steps: one to amplify the regions of interest, followed by a second PCR reaction to add adapter sequences (indexes) for NGS. While these methods are well established there is always a high risk of cross contamination or carry-over from first to second rounds of amplification.

RC-PCR eliminates risk of cross-contamination and pipetting error

Reverse Complement PCR (RC-PCR) is the only single step NGS Library Prep method as simple as a single PCR, combining indexing and PCR amplification in a single tube and reaction. NGS Library Prep will never be any simpler and safer. See the video animation of the working principle below.


Legal Notice:

RC-PCR is IP protected.

US Patent / WO Patent / EP Patent


Exclusive license granted to NimaGen B.V., Nijmegen, The Netherlands by SALISBURY NHS FOUNDATION TRUST (SALISBURY, WILTSHIRE, GB)


It is strictly prohibited to use, produce, distribute, import, or sell RC-PCR based methods or kits without the patent owner's written consent.

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Working principle

How Reverse Complement PCR Works


Easy Handling

Just one Pre-PCR reaction setup, quick, easy and highly suited for automation


Product Generation is More Target Specific

Traditional PCR reactions have high concentrations of primer at the beginning of the reaction when the template availability is low. 

In a RC-PCR the target specific primer is synthesized during the reaction and consequently more aligned with the target availability reducing the chance for dimerization and off target binding.

Multiplex Capabilities

Predesigned panels available, targeting up to 100 amplicons in a single closed tube reaction.

Solutions available
  • EasySeq™ Human WES WGS Sample Tracking
  • IDseek® OmniSTR
  • IDseek® mYSTR
  • IDseek® SNP-ID
  • IDseek® Mitochondrial HVR
  • IDseek® Mitochondrial Full Genome Sequencing
  • EasySeq™ WGS SARS-CoV2
  • EasySeq™ 16S rRNA
  • EasySeq™ 18S + Cyp51A rRNA
  • EasySeq™ TP53 
  • EasySeq™ PALB2 + CHEK2 HS 
  • EasySeq™ BRCA 1/2 
  • EasySeq™ CFTR 
  • EasySeq™ CFTR Hotspot 
Custom made

Custom kits

NimaGen offers complete solutions for your custom NGS kits.

Please provide us with some info regarding your needs, and we will contact you as soon as possible.

Custom your own NGS kit