Conventional amplicon-based methods for NGS Library Prep target enrichment involve two PCR steps: one to amplify the regions of interest, followed by a second PCR reaction to add adapter sequences (indexes) for NGS. While these methods are well established there is always a high risk of cross contamination or carry-over from first to second rounds of amplification.
NGS Library Prep Contamination Concerns?
RC-PCR eliminates risk of cross-contamination and pipetting error
Reverse Complement PCR (RC-PCR) is the only single step NGS Library Prep method as simple as a single PCR, combining indexing and PCR amplification in a single tube and reaction. NGS Library Prep will never be any simpler and safer. See the video animation of the working principle below.
RC-PCR is IP protected.
US Patent / WO Patent / EP Patent
Exclusive license granted to NimaGen B.V., Nijmegen, The Netherlands by SALISBURY NHS FOUNDATION TRUST (SALISBURY, WILTSHIRE, GB)
It is strictly prohibited to use, produce, distribute, import, or sell RC-PCR based methods or kits without the patent owner's written consent.
How Reverse Complement PCR Works
Just one Pre-PCR reaction setup, quick, easy and highly suited for automation
Product Generation is More Target Specific
Traditional PCR reactions have high concentrations of primer at the beginning of the reaction when the template availability is low.
In a RC-PCR the target specific primer is synthesized during the reaction and consequently more aligned with the target availability reducing the chance for dimerization and off target binding.
Predesigned panels available, targeting up to 100 amplicons in a single closed tube reaction.
- EasySeq™ Human WES WGS Sample Tracking
- IDseek® OmniSTR
- IDseek® mYSTR
- IDseek® SNP-ID
- IDseek® Mitochondrial HVR
- IDseek® Mitochondrial Full Genome Sequencing
- EasySeq™ WGS SARS-CoV2
- EasySeq™ 16S rRNA
- EasySeq™ 18S + Cyp51A rRNA
- EasySeq™ TP53 (CE-IVD)
- EasySeq™ PALB2 + CHEK2 HS (CE-IVD)
- EasySeq™ BRCA 1/2 (CE-IVD)
- EasySeq™ CFTR (CE-IVD)
- EasySeq™ CFTR Hotspot (CE-IVD)
EasySeq™ NGS Library Prep by RC-PCR Products
EasySeq™ 18S and Cyp51A rRNA Fungal ID Kit, 96 rxn
EasySeq™ PALB2 Sequencing Kit, 96 rxn
EasySeq™ CFTR HotSpot Sequencing Kit, 96 rxn
EasySeq™ CFTR Sequencing Kit, 96 rxn
EasySeq™ BRCA1/2 + CHEK2 HS Sequencing Kit, 96 rxn
IDseek® mYSTR™ Y-Chromosomal STR Profiling Kit, 96 rxn
IDseek® OmniSTR™ Global Autosomal STR Profiling Kit, 96 rxn
IDseek® Mitochondrial DNA HVR Sequencing by MPS Kit, 96 rxn
IDseek® Mitochondrial DNA Full Genome Sequencing by MPS Kit, 96 rxn
IDseek® OmniSNP™ Identity Informative SNP Typing Kit, 96 rxn
EasySeq™ TP53 Sequencing Kit, 96 rxn
AmpliClean™ Cleanup Kit, Magnetic Beads (5 mL)
AmpliClean™ Cleanup Kit, Magnetic Beads (50 mL)
AmpliClean™ Cleanup Kit, Magnetic Beads (500 mL)
EasySeq™ SARS-CoV-2 WGS Library Prep Kit, 96 rxn
EasySeq™ SARS-CoV-2 WGS Library Prep Kit, 384 rxn
EasySeq™ Human WES WGS Sample Tracking Kit, 96 rxn
EasySeq™ 16S rRNA Bacterial ID Kit, 96 rxn
NimaGen offers complete solutions for your custom NGS kits.
Please provide us with some info regarding your needs, and we will contact you as soon as possible.