Conventional Amplicon based methods for NGS target enrichment involve two PCR steps – one to amplify the regions of interest followed by a second PCR reaction to add adapter sequences for NGS. While these methods are well established there is always a high risk of cross contamination or carry-over from first to second rounds of amplification.
Contamination Concerns?
Legal Notice:
RC-PCR is IP protected.
US Patent / WO Patent / EP Patent
Exclusive license granted to NimaGen B.V., Nijmegen, The Netherlands
by SALISBURY NHS FOUNDATION TRUST (SALISBURY, WILTSHIRE, GB)
It is strictly prohibited to use, produce, distribute, import, or sell RC-PCR based methods or kits without the patent owner's written consent.
How it Works
Easy Handling
Just one Pre-PCR reaction setup, quick, easy and highly suited for automation
Product Generation is More Target Specific
Traditional PCR reactions have high concentrations of primer at the beginning of the reaction when the template availability is low.
In a RC-PCR the target specific primer is synthesized during the reaction and consequently more aligned with the target availability reducing the chance for dimerization and off target binding.
Predesigned and custom panels available targeting up to 100 amplicons in a single closed tube reaction.
- Human Sample tracking and ID kit for WGS/WES QC
- Forensic Identification Kits
- Crop Genotyping assays (GreenoType)
- GMO detection kit
- Meat detection kit (including speciate)
- Pharmacogenetics (PGX)
- Circulating Tumour DNA kit
- Detection of aneuploidies
EasySeq NGS RC-PCR Products
EasySeq™ 18S and Cyp51A rRNA Fungal ID Kit, 96 rxn
EasySeq™ PALB2 Sequencing Kit, 96 rxn
EasySeq™ CFTR HotSpot Sequencing Kit, 96 rxn
EasySeq™ CFTR Sequencing Kit, 96 rxn
EasySeq™ BRCA1/2 + CHEK2 HS Sequencing Kit, 96 rxn
IDseek® mYSTR Y-Chromosomal 27 Short Tandem Repeat Kit, 96 rxn
IDseek® OmniSTR Global 30-plex Short Tandem Repeat Kit, 96 rxn
IDseek® Mitochondrial HVR Sequencing by NGS Kit, 96 rxn
IDseek® Mitochondrial Full Genome Sequencing by NGS Kit, 96 rxn
IDseek® SNP-ID 85-plex Kit, 96 rxn
EasySeq™ TP53 Sequencing Kit, 96 rxn
AmpliClean™ Cleanup Kit, Magnetic Beads (5 mL)
AmpliClean™ Cleanup Kit, Magnetic Beads (50 mL)
AmpliClean™ Cleanup Kit, Magnetic Beads (500 mL)
EasySeq™ SARS-CoV-2 WGS Library Prep Kit, 96 rxn
EasySeq™ SARS-CoV-2 WGS Library Prep Kit, 384 rxn
EasySeq™ Human Exome Sample Tracking Kit for NGS, 96 rxn
EasySeq™ 16S rRNA Bacterial ID Kit, 96 rxn
Custom kits
NimaGen offers complete solutions for your custom NGS kits.
Please provide us with some info regarding your needs, and we will contact you as soon as possible.