About PCR Purification and DyeTerminator Sequencing Cleanup

The iX-Pure™ DyeTerminator Cleanup Kit efficiently removes unincorporated dyeterminators and salt-ions from extension products cycle sequenced with BrilliantDye™ and BigDye™ chemistries. Resin-based clean-up is completed within 40 minutes. Purified dye-labeled extension products can be injected directly into Genetic Analyzers. The iX-Pure™ Dye Terminator Cleanup Kit is the proven drop-in alternative for BigDye XTerminator™.

BigDye and BigDye XTerminator are trademarks of Thermo Fisher Scientific.

The D-Pure™ DyeTerminator Cleanup Kit is the magnetic bead-based alternative to iX-Pure™ DyeTerminator or BigDye XTerminator™ and the high-quality alternative for CleanSEQ DyeTerminator Removal Kit.  Magnetic cleanup is completed directly on the cycle sequencing plate, either manually or automated. Purified products can be loaded directly on Genetic Analyzers without further resuspension. Compatible with BigDye XTerminator™ run modules.

BigDye and BigDye XTerminator are trademarks of Thermo Fisher Scientific. CleanSEQ is a trademark of Beckman Coulter.

ExS-Pure™ Enzymatic PCR Purification Kit removes excess primers and unincorporated nucleotides from the PCR reaction prior to sequencing, as any leftover primers can interfere with the strand-specific cycle sequencing reactions that will follow.  This kit provides a ready-for-use reagent containing a well-balanced mixture of two highly effective enzymes, recombinant hydrolytic and heat-labile enzymes, Exonuclease-I and Shrimp Alkaline Phosphatase. Prior to cycle sequencing, ExS-Pure™ is added directly to the PCR product, and cleanup is performed in a standard thermal cycler within 5 minutes. ExS-Pure™ is the widely adopted drop-in alternative to ExoSAP-IT™. 

ExoSAP-IT is a trademark of Thermo Fisher Scientific.

The AmpliClean™ Cleanup Kit provides highly efficient and cost-effective magnetic bead-based technology for high-throughput purification of PCR products.  AmpliClean™ removes, salts, primers, primer-dimers and dNTPs, leaving ultra-pure DNA of the desired length. It requires no centrifugation or vacuum filtration steps. Purified PCR products can be used directly for Sanger cycle sequencing.  The AmpliClean™ protocol is highly flexible for manual operation and can be easily incorporated in any liquid handling system for automated processing. AmpliClean™ magnetic beads are the widely adopted alternative to AMPure XP beads for purification.

AMPure XP is a trademark of Beckman Coulter.