Custom NGS panels: Tailor-made sequencing solutions 

When standard does not do the trick
Tailor-made sequencing for your unique targets.→ Design panelOne-tube workflow, Cost-efficient workflow→ See benefitsFree quote in five simple questions.→ Get quoteAny questions on custom NGS panels?→ See FAQ
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What if the sequencing kit you need doesn’t exist yet?

Standard panels force you to compromise, on coverage, cost, or flexibility. At NimaGen, we don’t.

 

With NG-Assay-on-Request™, we turn your unique targets into a fully validated, ready-to-use kit, designed around your specifications, not the other way around. How? By leveraging our Single Reaction Library Prep, which consolidates amplification, indexing, and library creation into one closed-tube reaction.

 

Applications include:

  • Sample Integrity Confirmation (SampleID)
  • Cell Line Authentication and contamination detection
  • Mutation Detection for Inherited Diseases
  • Ultra-sensitive detection of specific mutations in cfDNA/ctDNA
  • Microbiology / Microbiome / Infectious Disease
  • QA/QC in plant breeding applications
  • Animal Identification / Inheritance Checking

 

Want to know if it fits your needs? 

You’re just five simple questions away from a free, no-obligation quote. 

 

→ Answer 5 questions & get your quote 

NG-Assay-on-Request™: Your perfect panel 

NG-Assay-on-Request™ is built on a one-tube, multiplex, single reaction NGS library prep approach, enabled by Reverse Complement PCR technology. That combination means every sample runs through a single reaction from input to indexed library, with amplicon design validated entirely around your targets, species, and required sample volume. There is no RC-PCR experience required on your end. 

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Your needs → your design → your kit 

  • Submit – tell us your species, genome, targets, platform, and sample volume
  • Design – our R&D team handles creation of the panel, focusing on specificity and balance
  • Validate – wet-lab confirmation of on-target performance and coverage uniformity
  • Deliver – your ready-to-use kit, assembled and shipped

 

Whether you're a clinical lab prioritizing compliance and reliability, a research institute seeking flexibility and performance, or a core facility optimizing throughput and scalability, we tailor the process to your needs.

 

Sequencing. Simpler. Smarter. Safer. 

Order your custom panel quote today

"Excellent customer support"

NimaGen offered to develop a custom NGS targeted SNP Genotyping kit for our specific targets. NimaGen has proven to be a reliable and competent partner that delivers excellent customer support and fast progress. 

 

MVZ Labor Krone, Bad Salzuflen, Germany 

What your kit includes 

  • RC-PCR multiplex Probe Panel (designed for your targets)
  • RC-PCR HiFi Master Mix (optimized for your application)
  • RC-PCR Probe Dilution Buffer
  • Choice from 8 different pre-spotted Unique Dual Index Primer Plate – 96-well (UDI) 

Key benefits at a glance 

  • Multiplexing. Process dozens of targets in a single reaction
  • Fully customizable. Your targets, your species. Built from scratch around your exact specifications.
  • Cost-efficient workflow. Single-tube clean-up and optimal flow cell capacity
  • Choice of indexing strategy. UDI (96-well) or combinatorial indexing. Validated specs: ≥70% on-target reads, ≥95% coverage uniformity within 3-fold of the median. 
Free quote

Answer 5 questions & get your quote

Getting a quote is straightforward. Answer the 5 questions in the form and our team will come back with a tailored proposal within two to three business days. No commitment required.  

 

 

That’s it. No lengthy briefing documents, no upfront costs.

Submit your contact details and we will take it from there. We will also inform you promptly in case the requested design falls outside the scope of our technology. 

 

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Frequently asked questions (FAQ)

What is Reverse Complement PCR (RC-PCR) Technology?

Reverse Complement PCR (RC-PCR) is our patented, IP-protected technology, exclusively licensed to NimaGen B.V. by Salisbury NHS Foundation Trust. It powers our One-tube, Single Reaction NGS Library Prep, integrating amplification, indexing, and adapter ligation in a single closed reaction, unlike traditional PCR methods. Production, distribution, or use of RC-PCR-based methods or kits without written consent is strictly prohibited.

What are custom NGS panels? 

NG-Assay-on-Request™ provides fully customized NGS library prep kits for targeted sequencing, designed around your specific genomic targets rather than a generic catalogue selection. Built from scratch, or designed to extend the content of an existing panel. Free project consultation with our NGS experts is included, dedicated to a quick and practical turnaround. 

Why might standard NGS panels not meet my specific research needs?

Most commercial panels are built for the broadest possible market, which means they are rarely optimized for any single research context. 

 

Too narrow: Your variants of interest are absent from every available commercial panel, forcing you to accept incomplete coverage or invest months in an in-house design. 

 

Too broad: You pay for sequencing depth across targets that add no value to your study, driving up costs without improving the quality of your results. 

 

Time-consuming: Designing a custom panel in-house requires bioinformatics capacity for in silico probe design, wet-lab time for iterative validation, and expertise most diagnostic or research labs cannot spare, with development timelines that routinely stretch to several months. 

 

Unreliable: Self-designed panels introduce risk at every open-tube step, from probe design failures and uneven GC content to indexing errors and cross-contamination during library preparation. 

 

The kit should fit your science, not the other way around, and that is precisely the problem NG-Assay-on-Request™ was built to solve. 

What results can I expect from NG-Assay-on-Request™?

NG-Assay-on-Request™ delivers high on-target performance (≥70% reads), uniform coverage (≥95% within 3-fold of median), and reproducible results, all in a single reaction. Expect faster turnaround, fewer errors, and full traceability for your custom targets.

What applications and biological domains can NG-Assay-on-Request™ support?

NG-Assay-on-Request™ supports a wide range of applications across human, animal, plant, and microbiology domains, including hereditary diseases, cancer, forensic identification, breeding programs, QA/QC, pathogen detection, crop improvement, strain typing, and resistance analysis. If your target database exists, we can design a validated panel around it

How does the NG-Assay-on-Request™ process work?

The NG-Assay-on-Request™ process consists of four steps:

 

1. Submit your species, genome, targets, platform, and sample volume.

2. Design by our R&D team, ensuring specificity and balance.

3. Validate through wet-lab confirmation of on-target performance and coverage uniformity.

4. Deliver your ready-to-use kit, assembled and shipped.

 

This tailored process meets the needs of clinical labs, research institutes, and core facilities alike

How does NG-Assay-on-Request™ simplify NGS workflows?

NG-Assay-on-Request™ uses our Single Reaction Library Prep and Reverse Complement PCR to combine amplification, indexing, and library creation in one closed-tube reaction. This eliminates multi-step workflows, reduces contamination risk, and delivers sequencing-ready libraries faster, without requiring RC-PCR expertise.

 

No RC-PCR expertise? No problem. We’ve designed it so your targets, species, and sample volume drive the process, not the other way around

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