Nijmegen, The Netherlands, August 12, 2021
Following the overwhelming success of their EasySeq™ SARS-CoV-2 (novel coronavirus) WGS kit, NimaGen BV announces the further expansion of their RC-PCR based kit range with Forensic DNA applications.
SNPs, STRs and Mitochondrial sequencing
Short Tandem Repeat marker (STRs) analysis is the gold standard in forensics. DNA databases worldwide are built with STR data generated with a simple nomenclature convention, based on allele calling relative to known allelic ladders using fragment analysis on capillary electrophoresis systems. NimaGen’s IDseek® OmniSTaR Global (autosomal 30-plex STR kit) and mYSTR (Y Chromosome 27-plex STR kit) adds extra benefits over classical CE analysis, like information of the actual sequence in and around the repeat motifs and compatibility with highly degraded samples due to the smaller amplicons. It is sensitive and generates ready-to-sequence, indexed and tailed amplicons of common recommended markers, in one multiplex, closed-tube reaction. All amplicons are designed to meet the shortest possible fragment lengths and are compatible
with Illumina MiSeq systems, using 2 x 300 bp sequence runs with 2 x 10 bp Unique Dual Index reads. This combination of state-of-the-art features with the automated data analysis without human intervention makes this kit the ideal solution for the transition from classical electrophoresis to Massive Parallel Sequencing.
In addition to the STR kits, NimaGen offers also two types of Mitochondrial Sequencing kits (HV Region and full genome), and a SNP-ID 85-Plex system, especially designed for very highly degraded DNA, with amplicons down to 50 bp. See "Reverse complement-PCR, an innovative and effective method for multiplexing forensically relevant single nucleotide polymorphism marker systems" of Magdalena Bus et.al. (august 2021).
Part numbers and pricing will be available soon. NimaGen is looking for bèta test sites, and invites you to contact us if you are interested in a testkit.