Welcome
High-quality Dye Terminator Cycle sequencing kits are based on the gold-standard Sanger Chain Termination method.
ApplicationSanger Sequencing Cycle Sequencing kits and consumables for ABI Genetic Analyzers
Read moreApplicationNext Generation Sequencing RC-PCR and smMIP based targeted library prep solutions
Read moreApplicationGreenoTypeGenotyping and pathogen detection for Agri
Read moreApplicationAlpaqua Magnet PlatesMarket Leading, Innovative, high performance magnet plates with patented spring cushion technologyRead more
Premium kits and reagents for DNA Sequencing
Discover our brands
BrilliantDye
High-quality Dye Terminator Cycle sequencing kits are based on the gold-standard Sanger Chain Termination method.
Compatibility
Compatible with ABI genetic analyzers of the 310, 3100, 3130, 3500, 3730 and SeqStudio series. No need for changes
in protocol, settings or calibration.
NimaPOP
Polymer for capillary electrophoresis
Compatibility
Click-in bottles and vails for ABI genetic analyzers.
AmpliClean
A magnetic bead based clean-up method for the purification of Nucleic Acids. With its high flexibility it allows for standard cleanup and size selection.
Compatibility
Removes, salts, primers, primer-dimers and dNTPs, leaving ultra-pure DNA of the desired length. Purified products can be used directly for downstream applications.
D-Pure
A rapid, high performance dye-terminator removal process based on Solid Phase Reversible Immobilization (SPRI) technology.
Compatibility
Magnetic bead-based, low to high-throughput purification method for DNA sequencing reactions. No centrifugation or filtration steps required.
iX-Pure
DyeTerminator Cleanup kit for removing unwanted components such as salt ions, unincorporated dye terminators and dNTPs. Preventing their co-injection with your sequencing products.
Compatibility
Straightforward protocol, compatible with BigDye XTerminator run modules.
ExS-Pure
Eliminate all unwanted dNTP’s and residual primers from your PCR products.
Compatibility
Minimal hands-on time: just add ExS-Pure to your PCR product and the cleanup is performed in a standard thermal cycler in 5 minutes.
EasySeq Targeted Capture
Straightforward, one-step capturing and subsequently sample tagging for NGS sequencing.
Compatibility
Using sequence capturing with single molecule Molecular Inversion Probes, the system enables sequencing of the targeted regions in a very simple, fast and robust workflow, resulting in coverage with double probe tiling and independent reading of both strands. The resulting libraries are compatible with Illumina sequencing platforms.
RC-PCR
A new & novel Amplicon based targeted enrichment method for Next Generation Sequencing.
Reduce risk of cross contamination
Amplification and Barcode Indexing in 1 PCR Step.
GreenoType
NimaGen’s targeted Genotyping by NGS method, dedicated to the agriculture market.
Compatibility
GreenoType accelerates breeding programmes and Quality Assurance, while being very cost-effective compared to other technologies like two-step PCR library prep, KASP, TaqMan, Fluidigm, ddPCR.
Alpaqua
High performance magnet plates for a wide variety of applications.
Compatibility
Strong ring magnets and integrated spring cushion technology enable automation-friendly, high performance magnet bead separation.
Upcoming
Circulating Tumor DNA
Under development
NimaGen is currently validating a multiplex RC-PCR based assay for detection of hotspot mutations in circulating (cell-free) tumor DNA. With very low noise and high sensitivity, RC-PCR is the ultimate choice for this application, by preventing any kind of PCR cross contamination and immediate indexing step. More info to be followed soon. For request on performance, assay, content or interest to become a beta test or collaborator, please contact us
Targets included
EGFR p.G719 / p.T790M / p.C797S p.L858R / p.L861Q
EGFR insertions in exon 20 (p.V769 - D770ins / p.L747_T751ins / p.L747_P753ins)
EGFR deletions in exon 19 (deletions c.2235_2258 / p.745_753del)
KRAS p.G12 / p.G13 and p.Q61H
BRAF p.V600E
Upcoming
Human Identification
SNPs, STR and Mitochonrial
The Center for Human Identification at the University of North Texas Health Science Center in Fort Worth, TX, USA, developed in collaboration with NimaGen a customized RC-PCR SNP method for genotyping highly degraded DNA at ultral low concentration.
More information
Bone remains of several hundred year old could be genotyped with this method. Click on the link to view the abstract: Click here
Next to single-reaction / closed-tube SNP genotyping assays for Human Identification, NimaGen is also working on STR amplification for NGS and Mitochondrial sequencing.
Please contact us if you would be interested to have more information on our development in Human Identification applications
Product news
Latest news
News | 31.10.19
The European Human Genetics Conference 2020
NimaGen will be exhibiting at ESHG. June 6-9, 2020 in Berlin. Germany
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News | 08.09.19
International Plant & Animal Genome XXVIII
NimaGen will be exhibiting at PAG XXVIII January 11-15, 2020 in San Diego.
Read more
News | 12.05.19
Now available: Catalyst magnet plates
Designed for manual pipetting, this magnet plate pulls the beads to the sides, leaving space for the user to simply slide in their pipet tip without having to worry about disturbing the bead ring.
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News | 14.01.19
RC-PCR for NGS library generation
NimaGen B.V. today announce the agreement of licensing terms for a novel One Step PCR technology employing reverse complement probes from the Salisbury NHS Foundation Trust, of Salisbury, United Kingdom.
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