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Innovators in DNA tech

BrilliantDyeNimaPOPAmpliCleanD-PureiX-PureExS-PureEasySeq NGS Targeted CaptureEasySeq NGS RC-PCRGreenoTypeAlpaqua Magnet PlatesRead more
Premium kits and reagents for DNA Sequencing

Discover our brands

Our Brands

BrilliantDye


NimaPOP


AmpliClean


D-Pure


iX-Pure


ExS-Pure


EasySeq NGS Targeted Capture


EasySeq NGS RC-PCR


GreenoType


Alpaqua Magnet Plates

BrilliantDye

High-quality Dye Terminator Cycle sequencing kits are based on the gold-standard Sanger Chain Termination method.

Compatibility

Compatible with ABI genetic analyzers of the 310, 3100, 3130, 3500, 3730 and SeqStudio series. No need for changes
in protocol, settings or calibration.

NimaPOP

Polymer for capillary electrophoresis

Compatibility

Click-in bottles and vails for ABI genetic analyzers.

AmpliClean

A magnetic bead based clean-up method for the purification of Nucleic Acids. With its high flexibility it allows for standard cleanup and size selection.

Compatibility

Removes, salts, primers, primer-dimers and dNTPs, leaving ultra-pure DNA of the desired length. Purified products can be used directly for downstream applications.

D-Pure

A rapid, high performance dye-terminator removal process based on Solid Phase Reversible Immobilization (SPRI) technology.

Compatibility

Magnetic bead-based, low to high-throughput purification method for DNA sequencing reactions. No centrifugation or filtration steps required.

iX-Pure

DyeTerminator Cleanup kit for removing unwanted components such as salt ions, unincorporated dye terminators and dNTPs. Preventing their co-injection with your sequencing products.

Compatibility

Straightforward protocol, compatible with BigDye XTerminator run modules.

ExS-Pure

Eliminate all unwanted dNTP’s and residual primers from your PCR products.

Compatibility

Minimal hands-on time: just add ExS-Pure to your PCR product and the cleanup is performed in a standard thermal cycler in 5 minutes.

EasySeq Targeted Capture

Straightforward, one-step capturing and subsequently sample tagging for NGS sequencing.

Compatibility

Using sequence capturing with single molecule Molecular Inversion Probes, the system enables sequencing of the targeted regions in a very simple, fast and robust workflow, resulting in coverage with double probe tiling and independent reading of both strands. The resulting libraries are compatible with Illumina sequencing platforms.

RC-PCR

A new & novel Amplicon based targeted enrichment method for Next Generation Sequencing.

Reduce risk of cross contamination

Amplification and Barcode Indexing in 1 PCR Step.

GreenoType

NimaGen’s targeted Genotyping by NGS method, dedicated to the agriculture market.

Compatibility

GreenoType accelerates breeding programmes and Quality Assurance, while being very cost-effective compared to other technologies like two-step PCR library prep, KASP, TaqMan, Fluidigm, ddPCR.

Alpaqua

High performance magnet plates for a wide variety of applications.

Compatibility

Strong ring magnets and integrated spring cushion technology enable automation-friendly, high performance magnet bead separation.

Premium kits and reagents for DNA Sequencing

Discover our brands

BrilliantDye

High-quality Dye Terminator Cycle sequencing kits are based on the gold-standard Sanger Chain Termination method.

Compatibility

Compatible with ABI genetic analyzers of the 310, 3100, 3130, 3500, 3730 and SeqStudio series. No need for changes
in protocol, settings or calibration.

NimaPOP

Polymer for capillary electrophoresis

Compatibility

Click-in bottles and vails for ABI genetic analyzers.

AmpliClean

A magnetic bead based clean-up method for the purification of Nucleic Acids. With its high flexibility it allows for standard cleanup and size selection.

Compatibility

Removes, salts, primers, primer-dimers and dNTPs, leaving ultra-pure DNA of the desired length. Purified products can be used directly for downstream applications.

D-Pure

A rapid, high performance dye-terminator removal process based on Solid Phase Reversible Immobilization (SPRI) technology.

Compatibility

Magnetic bead-based, low to high-throughput purification method for DNA sequencing reactions. No centrifugation or filtration steps required.

iX-Pure

DyeTerminator Cleanup kit for removing unwanted components such as salt ions, unincorporated dye terminators and dNTPs. Preventing their co-injection with your sequencing products.

Compatibility

Straightforward protocol, compatible with BigDye XTerminator run modules.

ExS-Pure

Eliminate all unwanted dNTP’s and residual primers from your PCR products.

Compatibility

Minimal hands-on time: just add ExS-Pure to your PCR product and the cleanup is performed in a standard thermal cycler in 5 minutes.

EasySeq Targeted Capture

Straightforward, one-step capturing and subsequently sample tagging for NGS sequencing.

Compatibility

Using sequence capturing with single molecule Molecular Inversion Probes, the system enables sequencing of the targeted regions in a very simple, fast and robust workflow, resulting in coverage with double probe tiling and independent reading of both strands. The resulting libraries are compatible with Illumina sequencing platforms.

RC-PCR

A new & novel Amplicon based targeted enrichment method for Next Generation Sequencing.

Reduce risk of cross contamination

Amplification and Barcode Indexing in 1 PCR Step.

GreenoType

NimaGen’s targeted Genotyping by NGS method, dedicated to the agriculture market.

Compatibility

GreenoType accelerates breeding programmes and Quality Assurance, while being very cost-effective compared to other technologies like two-step PCR library prep, KASP, TaqMan, Fluidigm, ddPCR.

Alpaqua

High performance magnet plates for a wide variety of applications.

Compatibility

Strong ring magnets and integrated spring cushion technology enable automation-friendly, high performance magnet bead separation.

Upcoming

Circulating Tumor DNA

Under development

NimaGen is currently validating a multiplex RC-PCR based assay for detection of hotspot mutations in circulating (cell-free) tumor DNA. With very low noise and high sensitivity, RC-PCR is the ultimate choice for this application,  by preventing any kind of PCR cross contamination and immediate indexing step. More info to be followed soon. For request on performance, assay, content or interest to become a beta test or collaborator, please contact us

Targets included

EGFR p.G719 / p.T790M / p.C797S p.L858R / p.L861Q
EGFR insertions in exon 20 (p.V769 - D770ins / p.L747_T751ins / p.L747_P753ins)
EGFR deletions in exon 19 (deletions c.2235_2258 / p.745_753del)
KRAS p.G12 / p.G13 and p.Q61H
BRAF p.V600E

Upcoming

Human Identification

SNPs, STR and Mitochonrial

The Center for Human Identification at the University of North Texas Health Science Center in Fort Worth, TX, USA, developed in collaboration with NimaGen a customized RC-PCR SNP method for genotyping highly degraded DNA at ultral low concentration. 

 

More information

Bone remains of several hundred year old could be genotyped with this method.  Click on the link to view the abstract: Click here

Next to single-reaction / closed-tube SNP genotyping assays for Human Identification, NimaGen is also working on STR amplification for NGS and Mitochondrial sequencing.

Please contact us if you would be interested to have more information on our development in Human Identification applications