Single pre-PCR reaction setup - Just add cDNA and Mastermix to UDI index plates and go. Post- PCR pool all samples, perform single clean-up reaction, QC, quantify and sequence.
Less handling steps, less risk of contamination or sample mix up, greater safety and reassurance.
High-quality Dye Terminator Cycle sequencing kits are based on the gold-standard Sanger Chain Termination method.
Compatible with ABI genetic analyzers of the 310, 3100, 3130, 3500, 3730 and SeqStudio series. No need for changes
in protocol, settings or calibration.
Polymer for capillary electrophoresis
Click-in bottles and vails for ABI genetic analyzers.
A magnetic bead based clean-up method for the purification of Nucleic Acids. With its high flexibility it allows for standard cleanup and size selection.
Removes, salts, primers, primer-dimers and dNTPs, leaving ultra-pure DNA of the desired length. Purified products can be used directly for downstream applications.
A rapid, high performance dye-terminator removal process based on Magnetic Beads.
Magnetic bead-based, low to high-throughput purification method for DNA sequencing reactions. No centrifugation or filtration steps required.
DyeTerminator Cleanup kit for removing unwanted components such as salt ions, unincorporated dye terminators and dNTPs. Preventing their co-injection with your sequencing products.
Straightforward protocol, compatible with BigDye XTerminator run modules.
Eliminate all unwanted dNTP’s and residual primers from your PCR products.
Minimal hands-on time: just add ExS-Pure to your PCR product and the cleanup is performed in a standard thermal cycler in 5 minutes.
Straightforward, one-step capturing and subsequently sample tagging for NGS sequencing.
Using sequence capturing with single molecule Molecular Inversion Probes, the system enables sequencing of the targeted regions in a very simple, fast and robust workflow, resulting in coverage with double probe tiling and independent reading of both strands. The resulting libraries are compatible with Illumina sequencing platforms.
A new & novel Amplicon based targeted enrichment method for Next Generation Sequencing.
Amplification and Barcode Indexing in 1 PCR Step.
NimaGen’s targeted Genotyping by NGS method, dedicated to the agriculture market.
GreenoType accelerates breeding programmes and Quality Assurance, while being very cost-effective compared to other technologies like two-step PCR library prep, KASP, TaqMan, Fluidigm, ddPCR.
High performance magnet plates for a wide variety of applications.
Strong ring magnets and integrated spring cushion technology enable automation-friendly, high performance magnet bead separation.
NimaGen is currently validating a multiplex RC-PCR based assay for detection of hotspot mutations in circulating (cell-free) tumor DNA. With very low noise and high sensitivity, RC-PCR is the ultimate choice for this application, by preventing any kind of PCR cross contamination and immediate indexing step. More info to be followed soon. For request on performance, assay, content or interest to become a beta test or collaborator, please contact us. For more information on the assay and it's benefits, please click HERE
EGFR p.G719 / p.T790M / p.C797S p.L858R / p.L861Q
EGFR insertions in exon 20 (p.V769 - D770ins / p.L747_T751ins / p.L747_P753ins)
EGFR deletions in exon 19 (deletions c.2235_2258 / p.745_753del)
KRAS p.G12 / p.G13 and p.Q61H
BRAF p.V600E
The Center for Human Identification at the University of North Texas Health Science Center in Fort Worth, TX, USA, developed in collaboration with NimaGen a customized RC-PCR SNP method for genotyping highly degraded DNA at ultra low concentration.
Bone remains of several hundred year old could be genotyped with this method. Click on the link to view the abstract: Click here
Next to single-reaction / closed-tube SNP genotyping assays for Human Identification, NimaGen is also working on STR amplification for NGS and Mitochondrial sequencing.
Please contact us if you would be interested to have more information on our development in Human Identification applications
