Innovators in DNA Sequencing Technologies

EasySeq™ NGS Library Prep by Reverse Complement PCR: The Safest, The Fastest, The Simplest Method Available

Fields of Application & Techniques

COVID-19

Forensics

Sanger Sequencing

Alpaqua® Magnet Plates

Microbiology & Infectious Disease

Human Genetics

EasySeq™ NGS Library Prep

NimaGen Product Highlights

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COVID-19

Key contributor to fighting the COVID-19 pandemic: EasySeq™ RC-PCR SARS CoV-2 (novel coronavirus) WGS generates sequencing data from cDNA, derived from positive tested patients and wastewaters.

IDseek® Forensic Applications

IDseek® MPS Library Prep (EasySeq™ workflow based) using patented, high-sensitive Reverse Complement PCR. A complete range of panels for Forensic DNA analysis on illumina® Sequencing Platforms.

Sanger Sequencing and Fragment Analysis by Capillary Electrophoresis

A full range of widely adopted quality reagents for ABI Genetic Analyzer based Capillary Electrophoresis, e.g. BrilliantDye™, NimaPOP, D-Pure™, iX-Pure™, ExS-Pure™, and Red and Orange DNA Size Standards.

Alpaqua® Magnet Plates

The most frequently recommended and widely adopted magnet plates for nucleic acid extraction, purification and NGS Library construction and exome capture. For manual and automated protocols.

Microbiology & Infectious Disease

EasySeq™ for identification, classification, and surveillance of bacteria, fungi and viruses, to help improve clinical and public health decisions associated with humans, animals, food, and environment.

Human Genetics

EasySeq™ NGS Library Prep by Reverse Complement PCR: the simplest, fastest and safest method for targeted DNA (re)sequencing of genes associated with cancers, Cystic Fibrosis and CFTR-related disorders.

BrilliantDye™ Terminator (v3.1) Cycle Sequencing Kit (100 rxn)

EasySeq™ Human Exome Sample Tracking Kit for NGS, 96 rxn

IDseek® OmniSTR Global 30-plex Short Tandem Repeat Kit, 96 rxn

Alpaqua MAGNUM FLX® Enhanced Universal Magnet Plate

EasySeq™ 16S rRNA Bacterial ID Kit, 96 rxn

Discover our Premium Solutions for DNA Sequencing

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EasySeq™ SARS-CoV-2 WGS Library Prep Kit, 96 rxn

BrilliantDye™ Terminator (v3.1) Cycle Sequencing Kit (100 rxn)

EasySeq™ Human Exome Sample Tracking Kit for NGS, 96 rxn

IDseek® OmniSTR Global 30-plex Short Tandem Repeat Kit, 96 rxn

Alpaqua MAGNUM FLX® Enhanced Universal Magnet Plate

EasySeq™ 16S rRNA Bacterial ID Kit, 96 rxn

NGS-driven SARS-CoV-2 Surveillance

Revolutionary NGS Library Prep by patented RC-PCR technology. Generate Whole Genome Sequencing data from cDNA, derived from positive tested patients, providing efficient classification of the SARS-CoV-2 genome.

illumina® Platform Compatibility

Compatible with any low- and medium-throughput illumina® NGS sequencer of the latest generation: iSeq™, MiniSeq™, MiSeq™, NextSeq™, in combination with 2x151 bp paired-end read chemistry.

Proven, Robust Chemistry

Generate data with uniform peak heights and optimized signal balance to produce long, high-quality reads.

Genetic Analyzer Compatibility

Widely adopted by labs using 310, 3100, 3130, 3500, 3730 Series Genetic Analyzers and SeqStudio™.

Tracking Sample Identity

NGS samples misidentification: a recognizable concern. Independent confirmation of sample identity is highly desirable, preferably using a method that is intrinsic in the WGS/WES sample data.

EasySeq™ HEST

EasySeq™ Human Exome Sample Tracking (HEST) Kit is a single-tube multiplex assay, containing an optimized panel of 34 SNPs and Amelogenin X and Y, to be utilized as intrinsic identifiers from human genomic DNA.

IDseek®: Forensic MPS revolution

IDseek® is the first range of MPS assays providing a more fast, safe and simple workflow, based on RC-PCR technology, with the potential to compete with CE-based assays, for routine application in forensic casework.

Kit Compatibility

All amplicons are designed to meet the shortest possible fragments lengths and are compatible with illumina® MiSeq™. This allows analysis of highly degraded samples, and sensitive detection of more STRs, avoiding the need for separate, secondary workflows in the lab.

Widely Adopted Alpaqua Technology

Alpaqua MAGNUM FLX® contains proprietary, solid-core ring magnets that enable fast separation of sample volumes up to 2 mL, and low volume nucleic acid elution down to 10 µl. SBS footprint, universally 96-well plate compatible.

Plate Compatibility

MAGNUM FLX® is compatible with all commonly used 96-well plate types: Flat-Bottom, Round-Bottom, V-Bottom, Deep-Well, or PCR plates (full, semi, or non-skirted). MAGNUM FLX® can be used in virtually all automated liquid handling decks, including those with deep plate nests.

Bacterial 16S Sequencing

Analyzing 16S rRNA via RC-PCR based targeted sequencing library prep provides new insights in identifying bacterial content (e.g. pathogens, antibiotic resistant strains) in challenging samples (animal, human, food, environment).

One-step Indexing and PCR

This 16S rRNA assay, based on RC-PCR, consists of the variable regions 1-2-3-4-5-6 and 9. Sequence ready libraries are generated from cDNA of the bacterial 16S rRNA, with minimal hands-on time and only two one-step RC-PCR reactions combining amplification and indexing.