Innovators in DNA Sequencing Technologies

EasySeq™ NGS Library Prep by Reverse Complement PCR: The Safest, The Fastest, The Simplest Method Available

Fields of Application & Techniques

IDseek® Forensic Applications

COVID-19 Sequencing

Sanger Sequencing

Alpaqua® Magnet Plates

Microbiology & Infectious Disease

Human Genetics

EasySeq™ NGS Library Prep

EasySeq™ NGS Sample Tracking

NimaGen Product Highlights

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COVID-19

Key contributor to fighting the COVID-19 pandemic: EasySeq™ RC-PCR SARS CoV-2 (novel coronavirus) WGS generates sequencing data from cDNA, derived from positive tested patients and wastewaters.

IDseek® Forensic Applications

IDseek® MPS Library Prep (EasySeq™ workflow based) using patented, high-sensitive Reverse Complement PCR. A complete range of STR, SNP and mtDNA panels for Forensic DNA analysis on illumina® platforms.

Sanger Sequencing and Fragment Analysis by Capillary Electrophoresis

A full range of widely adopted quality reagents for ABI Genetic Analyzer based Capillary Electrophoresis, e.g. BrilliantDye™, NimaPOP™, D-Pure™, iX-Pure™, ExS-Pure™, Seq-DI™, and Red/Orange DNA Size Standards.

Alpaqua® Magnet Plates

The most frequently recommended and widely adopted magnet plates for nucleic acid extraction, purification and NGS Library construction and exome capture. For manual and automated protocols.

Microbiology & Infectious Disease

EasySeq™ for identification, classification, and surveillance of bacteria, fungi and viruses, to help improve clinical and public health decisions associated with humans, animals, food, and environment.

Human Genetics

EasySeq™ NGS Library Prep by Reverse Complement PCR: the simplest, fastest and safest method for targeted DNA (re)sequencing of genes associated with cancers, Cystic Fibrosis and CFTR-related disorders.

BrilliantDye™ Terminator (v3.1) Cycle Sequencing Kit (100 rxn)

EasySeq™ Human WES WGS Sample Tracking Kit, 96 rxn

Alpaqua MAGNUM FLX® Enhanced Universal Magnet Plate

EasySeq™ 16S rRNA Bacterial ID Kit, 96 rxn

Discover our Premium Solutions for DNA Sequencing

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EasySeq™ SARS-CoV-2 WGS Library Prep Kit, 96 rxn

BrilliantDye™ Terminator (v3.1) Cycle Sequencing Kit (100 rxn)

EasySeq™ Human WES WGS Sample Tracking Kit, 96 rxn

IDseek® OmniSTR™ Global Autosomal STR Profiling Kit, 96 rxn

Alpaqua MAGNUM FLX® Enhanced Universal Magnet Plate

EasySeq™ 16S rRNA Bacterial ID Kit, 96 rxn

NGS-driven SARS-CoV-2 Surveillance

Revolutionary NGS Library Prep by patented RC-PCR technology. Generate Whole Genome Sequencing data from cDNA, derived from positive tested patients, providing efficient classification of the SARS-CoV-2 genome.

illumina® Platform Compatibility

Compatible with any low- and medium-throughput illumina® NGS sequencer of the latest generation: iSeq™, MiniSeq™, MiSeq™, NextSeq™, in combination with 2x151 bp paired-end read chemistry.

Proven, Robust Chemistry

Generate data with uniform peak heights and optimized signal balance to produce long, high-quality reads.

Genetic Analyzer Compatibility

Widely adopted by labs using 310, 3100, 3130, 3500, 3730 Series Genetic Analyzers and SeqStudio™.

Tracking Sample Identity

NGS samples misidentification: a recognizable concern. Independent confirmation of sample identity is highly desirable, preferably using a method that is intrinsic in the WES and WGS sample data.

EasySeq™ HST WES/WGS

EasySeq™ Human WES WGS Sample Tracking (HST WES/WGS) Kit is a single-tube multiplex assay, containing an optimized panel of 34 SNPs and Amelogenin X and Y, to be utilized as intrinsic identifiers from human genomic DNA.

IDseek®: Forensic MPS revolution

IDseek® is the first range of MPS assays providing a more safe and simple workflow, based on RC-PCR technology, with the potential to compete with CE-based assays, for routine application in forensic casework.

Kit Compatibility

Reverse Complement (RC-PCR) based MPS library prep solution for autosomal STRs relative to establishing identity of missing persons, confirm familial relations, and link persons of interest to crime scenes. Covering all relevant STR core loci from USA, EU, Germany, UK, and Interpol. All amplicons are designed to meet the shortest possible fragments lengths and are compatible with illumina® MiSeq™. This allows analysis of highly degraded samples, and sensitive detection of more STRs, avoiding the need for separate, secondary workflows in the lab.

Widely Adopted Alpaqua Technology

Alpaqua MAGNUM FLX® contains proprietary, solid-core ring magnets that enable fast separation of sample volumes up to 2 mL, and low volume nucleic acid elution down to 10 µl. SBS footprint, universally 96-well plate compatible.

Plate Compatibility

MAGNUM FLX® is compatible with all commonly used 96-well plate types: Flat-Bottom, Round-Bottom, V-Bottom, Deep-Well, or PCR plates (full, semi, or non-skirted). MAGNUM FLX® can be used in virtually all automated liquid handling decks, including those with deep plate nests.

Bacterial 16S Sequencing

Analyzing 16S rRNA via RC-PCR based targeted sequencing library prep provides new insights in identifying bacterial content (e.g. pathogens, antibiotic resistant strains) in challenging samples (animal, human, food, environment).

One-step Indexing and PCR

This 16S rRNA assay, based on RC-PCR, consists of the variable regions 1-2-3-4-5-6 and 9. Sequence ready libraries are generated from cDNA of the bacterial 16S rRNA, with minimal hands-on time and only two one-step RC-PCR reactions combining amplification and indexing.