No search results found. Try to search the complete website.
Reverse Complement (RC-PCR) based MPS library prep solution for autosomal STRs relative to establishing identity of missing persons, confirm familial relations, and link persons of interest to crime scenes. Covering all relevant STR core loci from USA, EU, Germany, UK, and Interpol.
RC-PCR provides multiplex targeted amplification, barcode indexing and adaptor addition. All steps are combined simultaneously in one single closed tube reaction.
All amplicons are designed to meet the shortest possible fragment lengths and are compatible with illumina® MiSeq™ and illumina® (Qiagen/Verogen) MiSeq™ FGx systems, with 2x 10 bp Unique Dual Index reads.
IDseek® Kits have been tested and validated for inhibition with Tannic Acid, Humic Acid, Hematin, Indigo and bacterial DNA . RC-PCR technology allows the reliable analysis of highly degraded, low quality / quantity samples frequently found in forensic casework.
IDseek® comprises a complete solution of reagents and consumables required for generating sequence-ready MPS libraries for Forensic applications.
IDseek® reagent kits for 96 reactions contain:
- RC-PCR Probe Mix
- RC-PCR HiFi 2x Hotstart PCR Mastermix
- Probe Dilution Buffer
To be ordered separately:
- Dehydrated Pre-spotted Unique Index (UDI) Primer Plate (choice of 4 different UDI plates), allowing for up to 384 samples per run,
- AmpliClean™ Cleanup kit, Magnetic Beads
A magnet compatible with 1.5 mL/2.0 mL centrifuge tubes is recommended for use with the AmpliClean™ Magnetic Bead Cleanup.
.jpg)
The OmniSTR™ Kit is a single reaction, multiplex library prep kit for autosomal STR profiling, including all the US and European expanded core loci, plus SE33, D4S2408, D6S1043, D9S1122, D17S1301, D20S482, PentaD, PentaE and DYS391. All targets have been designed for short amplicons, while maintaining the most informative sites in the flanking regions.
The combination of the latest advancements in MPS library prep, combined state-of-the-art features with the possibility for automated data analysis makes this kit the ideal solution for the transition from CE to MPS.