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IDseek® OmniSTR™ Global Autosomal STR Profiling Kit, 96 rxn

SKU: IDS-ASTR96
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Product Information

IDseek® OmniSTR™ Global Autosomal STR Profiling Kit
 

Intended use

Reverse Complement (RC-PCR) based MPS library prep solution for autosomal STRs relative to establishing identity of missing persons, confirm familial relations, and link persons of interest to crime scenes. Covering all relevant STR core loci from USA, EU, Germany, UK, and Interpol.

 

RC-PCR provides multiplex targeted amplification, barcode indexing and adaptor addition. All steps are combined simultaneously in one single closed tube reaction. 

Compatibility

All amplicons are designed to meet the shortest possible fragment lengths and are compatible with illumina® MiSeq™ and illumina® (Qiagen/Verogen) MiSeq™ FGx systems, with 2x 10 bp Unique Dual Index reads.

 

IDseek® Kits have been tested and validated for inhibition with Tannic Acid, Humic Acid, Hematin, Indigo and bacterial DNA . RC-PCR technology allows the reliable analysis of highly degraded, low quality / quantity samples frequently found in forensic casework.

Kit Components

IDseek® Reagents and Consumables

Sequence-ready libraries

IDseek® comprises a complete solution of reagents and consumables required for generating sequence-ready MPS libraries for Forensic applications. 

IDseek® components

IDseek® reagent kits for 96 reactions contain:

- RC-PCR Probe Mix
- RC-PCR HiFi 2x Hotstart PCR Mastermix 
- Probe Dilution Buffer 

To be ordered separately:

- Dehydrated Pre-spotted Unique Index (UDI) Primer Plate    (choice of 4 different UDI plates), allowing for up to 384 samples per run, 

- AmpliClean™ Cleanup kit, Magnetic Beads

A magnet compatible with 1.5 mL/2.0 mL centrifuge tubes is recommended for use with the AmpliClean™ Magnetic Bead Cleanup.

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The OmniSTR™ Kit is a single reaction, multiplex library prep kit for autosomal STR profiling, including all the US and European expanded core loci, plus SE33, D4S2408, D6S1043, D9S1122, D17S1301, D20S482, PentaD, PentaE and DYS391. All targets have been designed for short amplicons, while maintaining the most informative sites in the flanking regions.

The combination of the latest advancements in MPS library prep, combined state-of-the-art features with the possibility for automated data analysis makes this kit the ideal solution for the transition from CE to MPS.

Sensitive: 100% of markers called, down to 60 pg of DNA input
Marker balance optimized for 1 ng input DNA, with limited impact on low inputs down to 60 pg
High on-target read percentage, even with low input, minimizing sequencing costs
Robust and inhibitor tolerant, tested for Humic Acid, Tannic Acid, Indigo, Hematin and Bacterial DNA
News | 13.09.23

NimaGen x NicheVision Partnership Announcement 

NimaGen and NicheVision are partnering to deliver a complete wet-to-dry lab solution for forensic DNA analysis!     This partnership aims to provide forensic DNA laboratories with a combined cutting-edge DNA sequencing and data analysis workflow for human identification, that helps DNA analysts resolve complex DNA mixtures through a fully continuous approach.   The partnership is a distribution and development agreement that grants NicheVision exclusive rights to distribute NimaGen products for forensic DNA analysis in North, Central and South America. The companies will collaborate to commercialize NimaGen’s Reverse Complement PCR powered IDseek® Massively Parallel Sequencing (MPS) workflow in combination with NicheVision MixtureAce™ expert forensic DNA analysis software; as well as NimaGen brand products (NimaPOP™, NimaPrime™) for Genetic Analyzer based Fragment Analysis.  Come see us at ISHI 2023 on Sept 18-21st in Denver, CO! NimaGen will be at Booth #313 and NicheVision will be at Booth #312. Come learn all about the next revolution in Forensic MPS!
News | 16.08.23

Triolab AS (Norway): Welcome to the NimaGen Distributor Network!

Creating Shared Value Recently, NimaGen has initiated a new collaboration with Triolab AS in Norway. We look forward to strengthening our service to customers in this region together with Triolab as our distributor. Triolab is based in Kjeller, just north of Oslo, and will exclusively represent our Sanger-CE and NGS Library Preparation portfolio. They have a strong scientific team that will be able to support researchers with their knowledge and expertise.    Contact information:  Triolab AS Jogstadveien 21 2007 KJELLER Norway   Phone: 0047 63835750 Email: info@triolab.no Website: https://www.triolab.no/
News | 13.07.23

Radboud University & NimaGen: Genetic technique reveals pathogens much more accurately

''‘For example, in the Netherlands we monitor coronavirus in hospitals and wastewater, but we can easily expand that to other pathogens’, Coolen says. ‘In addition, we already apply this technique weekly in clinical diagnostics to detect so-called non-tuberculosis mycobacteria, a growing medical problem.''   ''Especially when looking for tiny amounts of a pathogen's DNA in a sea of contamination, such as in wastewater. Jordy Coolen, a PhD student at Radboud university medical center, came up with several solutions to implement sequencing in routine care. His work also included a collaboration with the company NimaGen. With relatively simple steps and lower costs, he was able to very sensitively analyze the genome of microorganisms.''   Link to Radboud University collaboration article: https://www.radboudumc.nl/en/news-items/2023/genetic-technique-reveals-pathogens-much-more-accurately
News | 03.07.23

IVD Bulgaria Ltd (Bulgaria ): Welcome to the NimaGen Distributor Network!

We are happy to announce that we strengthened the collaboration with IVD Bulgaria. Our companies have been working together for several years and we have now solidified this with a Distributor Agreement for Bulgaria. Based in Sofia, Bulgaria, the company focusses on distribution of in-vitro laboratory reagents and consumables, tests and equipment used in human and veterinary diagnostics. We are happy to work together in supporting customers in Bulgaria!