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IDseek® OmniSTR™ Global Autosomal STR Profiling Kit, 96 rxn

SKU: IDS-ASTR96
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Product Information

IDseek® OmniSTR™ Global Autosomal STR Profiling Kit
 

Intended use

Reverse Complement (RC-PCR) based MPS library prep solution for autosomal STRs relative to establishing identity of missing persons, confirm familial relations, and link persons of interest to crime scenes. Covering all relevant STR core loci from USA, EU, Germany, UK, and Interpol.

 

RC-PCR provides multiplex targeted amplification, barcode indexing and adaptor addition. All steps are combined simultaneously in one single closed tube reaction. 

Compatibility

All amplicons are designed to meet the shortest possible fragment lengths and are compatible with illumina® MiSeq™ and illumina® (Qiagen/Verogen) MiSeq™ FGx systems, with 2x 10 bp Unique Dual Index reads.

 

IDseek® Kits have been tested and validated for inhibition with Tannic Acid, Humic Acid, Hematin, Indigo and bacterial DNA . RC-PCR technology allows the reliable analysis of highly degraded, low quality / quantity samples frequently found in forensic casework.

Kit Components

IDseek® Reagents and Consumables

Sequence-ready libraries

IDseek® comprises a complete solution of reagents and consumables required for generating sequence-ready MPS libraries for Forensic applications. 

IDseek® components

IDseek® reagent kits for 96 reactions contain:

- RC-PCR Probe Mix
- RC-PCR HiFi 2x Hotstart PCR Mastermix 
- Probe Dilution Buffer 

To be ordered separately:

- Dehydrated Pre-spotted Unique Index (UDI) Primer Plate    (choice of 4 different UDI plates), allowing for up to 384 samples per run, 

- AmpliClean™ Cleanup kit, Magnetic Beads

A magnet compatible with 1.5 mL/2.0 mL centrifuge tubes is recommended for use with the AmpliClean™ Magnetic Bead Cleanup.

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The OmniSTR™ Kit is a single reaction, multiplex library prep kit for autosomal STR profiling, including all the US and European expanded core loci, plus SE33, D4S2408, D6S1043, D9S1122, D17S1301, D20S482, PentaD, PentaE and DYS391. All targets have been designed for short amplicons, while maintaining the most informative sites in the flanking regions.

The combination of the latest advancements in MPS library prep, combined state-of-the-art features with the possibility for automated data analysis makes this kit the ideal solution for the transition from CE to MPS.

Sensitive: 100% of markers called, down to 60 pg of DNA input
Marker balance optimized for 1 ng input DNA, with limited impact on low inputs down to 60 pg
High on-target read percentage, even with low input, minimizing sequencing costs
Robust and inhibitor tolerant, tested for Humic Acid, Tannic Acid, Indigo, Hematin and Bacterial DNA
News | 08.05.23

12th Haploid Markers Conference: Come Meet NimaGen's Pieter van Oers and learn all about IDseek®: The Next Revolution in Forensic MPS!

Following our attendance at ESWG ISFG 2023, NimaGen will have our representative Pieter van Oers present at the 12th Haploid Markers Conference in Budapest, Hungary from May 17th-20th, 2023. Come learn all about IDseek®, the next Revolution in Forensic MPS powered by RC-PCR technology!   Our NimaGen representative, Pieter van Oers, look forward to meeting you!   Interested? Learn more at www.nimagen.com/forensics or contact us directly at: www.nimagen.com/contact
News | 12.04.23

Advena (Kazakhstan, Uzbekistan, Kyrgyzstan, Armenia): Welcome to the NimaGen Distributor Network!

We are happy to share that we have set up a new collaboration with Advena LLP as of April 1st, 2023. Advena are now the exclusive representatives for the NimaGen portfolio in Kazakhstan, Uzbekistan, Kyrgyzstan and Armenia. The company is headquartered in Astana, Kazakhstan, and their team has a long history in distribution and Life Science. We are excited to be able to support our customers in these countries through the collaboration with Advena.