Reverse Complement (RC-PCR) based MPS library prep solution for analysis of hypervariable regions (mutation hotspots) in very low quantities of (highly degraded) nuclear mtDNA in complex samples that do not provide a full STR. For laboratories in those countries where full genome sequencing in forensics is restricted by guidelines or regulations.
RC-PCR provides multiplex targeted amplification, sequencing indexing and adaptor addition occuring simultaneously in a single closed tube reaction.