IDseek® Mitochondrial DNA HVR Kit 96 rxn
Description
The IDseek® Mitochondrial DNA HVR Sequencing by MPS Kit is a Reverse Complement (RC-PCR) based library preparation solution for analyzing the hypervariable regions in very low quantities of (highly degraded) nuclear mtDNA in complex samples that do not provide a full STR profile. The kit enables multiplex targeted amplification, sequencing indexing, and adaptor addition in a single closed-tube reaction.
The IDseek® Mitochondrial DNA HVR Kit is optimized for use with Illumina® MiSeq™ systems, utilizing 2x 10 bp Unique Dual Index reads. RC-PCR technology allows the reliable analysis of highly degraded samples frequently found in forensic casework.
Kit components
• Master Mix
• Probe Dilution Buffer
• Probe Panels
• Elution Buffer
Note: This item requires (a) dedicated Index Plate(s).
Once you’ve added this RC-PCR kit to your cart, you'll be prompted to select the appropriate Unique Dual Index Primer Plate(s).
Compatibility
• Compatible with Illumina® MiSeq™ systems.
Benefits
✓ Single-tube reaction for simplified workflow
✓ Minimized risk of contamination and errors
✓ RC-PCR kinetics enhance specificity and sensitivity
✓ Cost effective and high quality Mitochondrial DNA analysis
✓ Optimized for degraded and low-input DNA
This product is for research use only. Not for use in diagnostic procedures.