Product

IDseek® Mitochondrial DNA HVR Sequencing by MPS Kit, 96 rxn

SKU: IDS-HVR96
Variants
Required products
Automatically added to cart
IDseek® Mitochondrial DNA HVR Sequencing by MPS Kit, 96 rxn
Variants
Required products
Automatically added to cart
Product Information

IDseek® Mitochondrial DNA HVR Sequencing by MPS Kit

Intended use

Reverse Complement (RC-PCR) based MPS library prep solution for analysis of hypervariable regions (mutation hotspots) in very low quantities of (highly degraded) nuclear mtDNA in complex samples that do not provide a full STR. For laboratories in those countries where full genome sequencing in forensics is restricted by guidelines or regulations. 

 

RC-PCR provides multiplex targeted amplification, sequencing indexing and adaptor addition occuring simultaneously in a single closed tube reaction. 

Compatibility

All amplicons are designed to meet the shortest possible fragment lengths and are compatible with illumina® MiSeq™ systems, with 2x 10 bp Unique Dual Index reads.

 

IDseek® Kits have been tested with Tannic Acid and Humic Acid. RC-PCR technology allows the reliable analysis of highly degraded samples frequently found in forensic casework.

Kit Components

IDseek® Reagents and Consumables

Sequence-ready libraries

IDseek® comprises a complete solution of reagents and consumables required for generating sequence-ready MPS libraries for Forensic applications: from indexing to amplification, magnetic library clean-up and size selection.

IDseek® components

IDseek® reagents and consumables required for 96 reactions:

- RC-PCR Probe Mix
- RC-PCR HiFi 2x Hotstart PCR Mastermix
- 2x Dehydrated Pre-spotted Unique Index Primer Plates
- AmpliClean™ Cleanup, Magnetic Beads


A magnet compatible with 1.5 mL/2.0 mL centrifuge tubes is recommended for use with the AmpliClean™ Magnetic Bead Cleanup.

idseek logo image
News | 08.05.23

12th Haploid Markers Conference: Come Meet NimaGen's Pieter van Oers and learn all about IDseek®: The Next Revolution in Forensic MPS!

Following our attendance at ESWG ISFG 2023, NimaGen will have our representative Pieter van Oers present at the 12th Haploid Markers Conference in Budapest, Hungary from May 17th-20th, 2023. Come learn all about IDseek®, the next Revolution in Forensic MPS powered by RC-PCR technology!   Our NimaGen representative, Pieter van Oers, look forward to meeting you!   Interested? Learn more at www.nimagen.com/forensics or contact us directly at: www.nimagen.com/contact
News | 12.04.23

Advena (Kazakhstan, Uzbekistan, Kyrgyzstan, Armenia): Welcome to the NimaGen Distributor Network!

We are happy to share that we have set up a new collaboration with Advena LLP as of April 1st, 2023. Advena are now the exclusive representatives for the NimaGen portfolio in Kazakhstan, Uzbekistan, Kyrgyzstan and Armenia. The company is headquartered in Astana, Kazakhstan, and their team has a long history in distribution and Life Science. We are excited to be able to support our customers in these countries through the collaboration with Advena.