The D-Pure™ Dye Terminator Cleanup Kit efficiently removes unincorporated dye terminators and salt-ions from extension products cycle sequenced with BrilliantDye™ and BigDye™ chemistries. Magnetic clean-up is completed directly on the cycle sequencing plate and can be processed both manually and automated. D-Pure™ effectively eliminates the presence of dye-blobs, through selective binding and high washing efficiency, allowing for cleaner sequences and more confident signal reading.
Intended use
Rapid and Effective Magnetic Dye Terminator Cleanup
Introduction
Compatibility
D-Pure™ DyeTerminator Cleanup kits are widely adopted as proven, high-quality reagents for laboratories using 310, 3100, 3130, 3500, 3730 Series Genetic Analyzers and SeqStudio™, and are compatible with BigDye™ and BrilliantDye™ Cycle Sequencing (v1.1 and v3.1) Kits. Purified dye-labeled extension products can be loaded directly on the Genetic Analyzers without the need for resuspension.
D-Pure™ DyeTerminator Cleanup can be used in conjunction with Alpaqua® magnet plates, e.g. the 96R Ring Magnet Plate.
BigDye and and SeqStudio are trademarks of Applied Biosystems, LLC.
Features
- Fast, reliable, proven, reproducible
- Efficient removal of cycle sequencing reaction contaminants
- Excellent short and long fragment recovery
- Straightforward workflow, both manually and automated
- No liquid transfer: cycle sequencing and clean-up in a single plate
- No centrifugation or filtration steps
- Compatible with BigDye XTerminator™ run modules
Also available
Alpaqua® Magnet Plates
NimaGen offers a broad range of Alpaqua® magnet plates, the leading and most frequently recommended brand for magnetic separation devices.
Alpaqua® magnets are used in combination with D-Pure™ DyeTerminator Cleanup Kit, or other magnetic bead-based PCR product and cycle sequencing clean-up products, and with AmpliClean™ Cleanup Kit and similar products for preparation of sequence-ready NGS libraries.