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BrilliantDye™ Terminator Cycle Sequencing Kits and Buffers

Intended use

BrilliantDye™ comprises a range of Dye Terminator Cycle Sequencing reagents and buffers for performing fluorescence-based cycle sequencing, including de novo sequencing and resequencing of single- and double-stranded DNA, PCR fragments and plasmids, using Capillary Electrophoresis (CE).


BrilliantDye™ Terminator Cycle Sequencing kits contain all required reagent components (BrilliantDye Terminator, 5x sequencing buffer, pGEM control, M13 control primer) for the sequencing reaction in a ready reaction, pre-mixed format. 


BrilliantDye™ kits and buffers are widely adopted as proven, high-quality reagents for laboratories using 310, 3100, 3130, 3500, 3730 Series Genetic Analyzers and SeqStudio™ Flex.


BrilliantDye™ Terminator (v1.1 and v3.1) Cycle Sequencing Kits are the proven alternative to Thermo Fisher BigDye™ Terminator Cycle Sequencing Kits.

For optimal results

BrilliantDye™ Terminator Cycle Sequencing Kits offer robust, highly flexible chemistry, providing data with uniform peak heights and optimized signal balance, which results in longer, higher-quality sequencing reads. NimaGen has four kit formats available to meet these sequencing requirements:


BrilliantDye™ Terminator v1.1 Cycle Sequencing Kit is designed for specialty applications that require optimal basecalling adjacent to the primer.


BrilliantDye™ Terminator v3.1 Cycle Sequencing Kit is the method of choice for longest reads.


dGTP BrilliantDye™ Cycle Sequencing Kits (v1.1 and v3.1), for sequencing through G- and GT-rich templates, are designed and optimized for challenging sequence templates, by replacing the dITP with dGTP.


BigDye and SeqStudio are trademarks of Thermo Fisher Scientific. 

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