EasySeq™ NGS Library Prep by Reverse Complement PCR for Bacteriology, Mycology and Virology
Based on patented Reverse Complement PCR (RC-PCR) technology, providing simple and safer NGS Library Prep workflows combined with high assay sensitivity, EasySeq™ offers an ideal solution for public health, research, and industrial microbiology laboratories, even those lacking NGS experience.
To date, next generation sequencing (NGS) analysis in bacteriology, mycology and virology has demonstrated to help improve clinical and public health decisions through more accurate and rapid determination of the sources of infectious diseases, as well as the epidemiology and evolution of infectious pathogens in animals, food, and environment.
NGS Advantages in Microbiology and Infectious Disease
NGS enables personalized microbiology, standardization of methods and tracking of outbreak-specific clones. For example, our up-to-date SARS-CoV-2 kits are currently being used for wastewaster testing to track emerging variants:
With NGS, whole genome-sequencing, targeted next-generation sequencing and metagenomicss are possible. Our targeted next-generation sequencing EasySeq™ kits for 16S sequencing allow a comprehensive view of microbial communities, which allows for the identification of new pathogens. We have two versions of the 16S kit:
- EasySeq™ 16S rRNA V1-6 and V9 Bacterial ID Sequencing Kit
- Provides the complete analysis of the variable regions 1-6 and 9 of the bacterial 16S rRNA gene, which supports an NGS driven bacterial identification strategy for infectious disease, contamination investigation and root cause analysis testing. Analyzing hypervariable regions V1-V6 and V9 for mainly bacterial identification from clinical samples, for use with shorter amplicons in a two-pool multiplex reaction.
Genetic technique reveals pathogens much more accurately
''‘For example, in the Netherlands we monitor coronavirus in hospitals and wastewater, but we can easily expand that to other pathogens’, Coolen says. ‘In addition, we already apply this technique weekly in clinical diagnostics to detect so-called non-tuberculosis mycobacteria, a growing medical problem.''
''Especially when looking for tiny amounts of a pathogen's DNA in a sea of contamination, such as in wastewater. Jordy Coolen, a PhD student at Radboud university medical center, came up with several solutions to implement sequencing in routine care. His work also included a collaboration with the company NimaGen. With relatively simple steps and lower costs, he was able to very sensitively analyze the genome of microorganisms.''