EasySeq™ Microbiology & Infectious Disease

One-tube, single reaction library prep for simplified microbiology workflows.
16S Bacterial Identification Library Prep Kit (short-read)→ Identify and profile bacteriaFull-length 16S Library Prep Kit (long-read)→ Full-length 16S in 8 hrsITS Microbiome & DuoBiome Library Prep Kits (short-read)→ Profile fungi, bacteria, or bothTest EasySeq™ kits in your own microbiology workflow→ Request a sample kit
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Simplifying sequencing for microbiology and infectious disease

Sequencing should serve science, not slow it down. That belief drives everything NimaGen builds, and it defines EasySeq™, our family of NGS library prep kits for microbiology and infectious disease.

 

EasySeq™ is built on a one-tube, single reaction workflow, enabled by our proprietary Reverse Complement PCR (RC-PCR) technology. Instead of multiple reactions and repeated handling steps, target amplification, indexing and library preparation take place in a single closed-tube reaction. For laboratories working on bacterial identification, viral detection, or microbiome profiling, this means fewer steps, less risk of error, and results you can trust.

 

As NimaGen, innovators in DNA sequencing technologies since 2011, we exist to simplify what others complicate. EasySeq™ reflects that same principle, applied to microbiology.

 

Sequencing. Simpler. Smarter. Safer.

16S Bacterial Identification and Microbiome Library Prep

16S Bacterial Identification Library Prep Kit

EasySeq™ 16S Bacterial Identification Library Prep Kit provides the complete analysis of the variable regions V1-6 and V9 of the bacterial 16S rRNA gene, which supports an NGS driven bacterial identification strategy for infectious disease, contamination investigation and root cause analysis testing. NGS has become a proven and increasingly adopted tool in clinical and public health decision making. EasySeq™ 16S Bacterial Identification is aimed at analysis of mainly clinical samples, using a two-panel multiplex RC-PCR reaction.

 

→ Learn more about EasySeq™ 16S Bacterial Identification 

→ Request a sample kit

16S Microbiome Library Prep Kit

EasySeq™ 16S Microbiome Library Prep Kit for all major short-read platforms provides the complete analysis of the variable regions V3-V4 of the bacterial 16S rRNA gene, which supports an NGS driven strategy for deconvolution of bacterial communities, like the microbiome, using a singleplex RC-PCR reaction. To date, NGS analysis in bacteriology has demonstrated to help improve clinical and public health decisions through more accurate and rapid determination of the sources of infectious diseases, as well as the epidemiology and evolution of infectious pathogens in humans, animals, food, feed and environment. Integration of human, animal, and environment areas allows studying microbiomes from a One Health perspective.

 

→ Learn more about EasySeq™ 16S Microbiome

→ Request a sample kit

Test EasySeq™ on your own samples

Request test samples for bacterial, fungal, and combined microbiome workflows

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Full-length 16S Library Prep Kit 

Full-length 16S Library Prep Kit 

EasySeq™ Full-length 16S Library Prep Kit provides a complete analysis of the bacterial 16S rRNA gene, compatible with Oxford Nanopore Technologies® (ONT) platforms. The kit provides a high-sensitive, cost-efficient workflow to sequence the entire 16S rRNA gene to characterize bacteria up to species level and obtain a sample-to-result within 8 hours. As the ONT platform allows real-time sequencing, results can be obtained while sequencing is still ongoing. From DNA isolated of various sample types such as environmental, feed and food, human or animal derived samples (e.g. gut, skin, feces) without the need for culture. 

 

→ Learn more about EasySeq™ Full-length 16S 

→ Request a sample

AmpliClean™ Cleanup Kit, Magnetic Beads

The AmpliClean™ Cleanup Kit provides highly efficient and cost-effective magnetic bead-based technology for high-throughput purification and size selection of PCR-generated NGS libraries. AmpliClean™ removes salts, primers, primer-dimers and dNTPs, leaving ultra-pure DNA of the desired length. It requires no centrifugation or vacuum filtration steps. The AmpliClean™ protocol is highly flexible for manual operation and can be easily incorporated in any liquid handling system for automated processing. AmpliClean™ magnetic beads are the widely adopted alternative to AMPure XP beads for purification. (AMPure XP is a trademark of Beckman Coulter).

 

→ Learn more about AmpliClean™

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ITS Microbiome and DuoBiome Library Prep Kits

ITS Microbiome Library Prep

EasySeq™ ITS Microbiome Library Prep Kit enables reliable profiling of fungal communities on short-read sequencing platforms. In a single reaction, target amplification, indexing and library preparation are combined in one tube, enabled by our proprietary Reverse Complement PCR technology, supporting multiplexing of up to 768 samples per run. The kit works directly on DNA from environmental, soil, feed, food, human and animal-derived samples.

 

→ Learn more about EasySeq™ ITS Microbiome

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DuoBiome Library Prep Kit

EasySeq™ DuoBiome Library Prep Kit combines the EasySeq™ 16S Microbiome and EasySeq™ ITS Microbiome workflows in one kit format, enabling bacterial and fungal microbiome profiling from the same sample in a single library preparation session. It supports separate analysis of the 16S V3-V4 region for bacteria and the ITS2 region for fungi, each amplified, indexed and prepared for sequencing in its own closed-tube reaction, with separate input and cycling conditions suited to complex sample types such as fecal material. After QC, both libraries are pooled equimolarly for sequencing on short-read platforms, supporting multiplexing of up to 768 samples.

 

→ Learn more about EasySeq™ DuoBiome

→ Request a sample

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Built on a one-tube, single reaction workflow

EasySeq™ combines target amplification, indexing and library preparation in a single closed-tube reaction, enabled by our proprietary Reverse Complement PCR (RC-PCR) technology. This one-tube, single reaction workflow supports the assay sensitivity, specificity and discriminatory power required in microbiology analysis

EasySeq™ NGS library prep offers a cost-efficient workflow, confidence in test results, and flexibility relative to laboratory workload and resources. It is suited to public health, research, and industrial microbiology laboratories, including those without prior NGS experience.

Learn more about Reverse Complement PCR (RC-PCR)

Test how EasySeq™ microbiology kits perform in your workflow.

From bacterial identification and 16S or fungal ITS microbiome profiling to combined bacterial-fungal profiling with DuoBiome, we provide a tailored test setup based on your application, sample type and sequencing platform. After submission, our team will contact you to align on your requirements. 

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