All species of archaea and bacteria have a 16S gene that codes for the small subunit of the ribosomes. This gene has highly variable regions (V1 to V9) used for taxonomical classifications. The gene also has conserved regions that can be used as targets for primers to amplify the highly variable regions.
Through introduction of Next-generation Sequencing (NGS), the 16S rRNA method is now also widely used to identify one or multiple bacterial species in a single sample.
EasySeq™ 16S Bacterial Identification Library Prep Kit, powered by Reverse Complement PCR (RC-PCR), provides a highly sensitive, cost-effective way to identify bacterial species in culture positive and culture-negative samples through analysis of hypervariable regions V1-V6 and V9. It can be used to decipher polymicrobial samples where traditional Sanger sequencing fails. Furthermore, resources and time are saved because the kit does not require culture and can be used with DNA extracted from many different sample types.