Product

EasySeq™ 16S Bacterial Identification Library Prep Kit, 2 pools/sample, incl. PCR Master Mix, 96 rxn

SKU: RC-16S096
Variants
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EasySeq™ 16S Bacterial Identification
Variants
Required products
Automatically added to cart
Product Information

16S rRNA V1-V6 and V9 for Bacterial Identification

Intended use

All species of archaea and bacteria have a 16S gene that codes for the small subunit of the ribosomes. This gene has highly variable regions (V1 to V9) used for taxonomical classifications. The gene also has conserved regions that can be used as targets for primers to amplify the highly variable regions.

 

Through introduction of Next-generation Sequencing (NGS), the 16S rRNA method is now also widely used to identify one or multiple bacterial species in a single sample.

 

EasySeq™ 16S Bacterial Identification Library Prep Kit, powered by Reverse Complement PCR (RC-PCR), provides a highly sensitive, cost-effective way to identify bacterial species in culture positive and culture-negative samples through analysis of hypervariable regions V1-V6 and V9. It can be used to decipher polymicrobial samples where traditional Sanger sequencing fails. Furthermore, resources and time are saved because the kit does not require culture and can be used with DNA extracted from many different sample types. 

Compatibility

The multiplex RC-PCR generates six amplicons (V1-2, V3, V4, V5, V6 and V9) that can be sequenced on Illumina® platforms iSeq-100, MiniSeq, MiSeq, NextSeq 500/550 and NextSeq 1000/2000. Depending on the platform, up to 768 samples can be multiplexed.

 

Please note: EasySeq™ 16S Bacterial Identification Library Prep Kit is designed to sequence the hypervariable regions V1-V6 and V9 in a multiplex reaction using two different probe panels. This requires two Unique Dual Index Plates for associated use. 

 

The EasySeq™ 16S Bacterial Identification Library Prep Kit allows direct amplification of DNA from clinical materials. The kit can also be used with DNA extracted from pure cultures.

Premium features
Downloads
Premium features

Cost-efficient Workflow - Confidence in Test Results - Choice in Sequencing Platform and Workload

One closed-tube, single reaction workflow with simultaneous indexing and target amplification reduce hands-on time, pipetting error and minimizes risk of sample contamination
Pre-spotted and breakable Unique Dual Index plates ensure accuracy, match your sample workload and ensure optimal usage, minimizing waste
Single tube sample pooling for library clean-up significantly reduces usage of required magnetic beads and consumables
Unique RC-PCR kinetics promote high-target specificity, sensitivity and coverage uniformity (optimized read depth balance) from low DNA input
Well-balanced read distributions and use of phase-shifted primers reduces PhiX spike-in, maximizing sequencing instrument flow cell capacity
Compatible with various Illumina® platforms and liquid handling systems for medium to high-throughput workflows

Publications 

''RC-PCR is an ideal compromise between the standard one-fragment approach and metagenomics sequencing and may guide future environmental sequencing studies, in which bacterial diversity is a central subject.'' 

 

Read more: https://ami-journals.onlinelibrary.wiley.com/doi/epdf/10.1111/1462-2920.16530

- Leontidou et al. (2023)
News | 08.01.24

NimaGen's 2023 Highlights ReCAPPed!

NimaGen had a great 2023 and were looking forward to an even better 2024!    NimaGen’s 2023 ReCAPPed™ (Yes, this a pun on our product name):  Our New Building and Facilities:  We kicked off 2023 by moving into our new building with state-of-the-art laboratory and production spaces, as well as comfortable office and meeting spaces! Our NimaGen Grand Opening party in May was an exciting event celebrated by all to commemorate such a momentous occasion; it was also attended by our local major of Nijmegen!            Our New Reverse Complement PCR Sequencing Kits:  From there, we spent the year developing several innovative and exciting new Reverse-Complement PCR sequencing kits for our application areas in Forensics, Microbiology and Infectious Disease and Human Genetics!      Distributors, Partnerships and Collaborations In 2023, NimaGen gained 15 partnerships around the world that help support our expanding product portfolio! From the far reaches of Barcode Biosciences in India to our neighboring country France supported by Dominique Dutscher SAS, as well as strategic collaborations with partnerships in North American with NicheVision for our Forensics portfolio, we desire to help our customers in every cornerstone of the world. Together, we will continue creating shared value!    Customer Experience Survey Rating At the end of 2023, active NimaGen customers received a customer experience survey with an average score of >8/10!. NimaGen responsiveness, solutions focus, personal interaction and product value were highly rated by both customers and distributors. We are grateful for the feedback we received and will continue to strive to continue improve our customer service, quality products and continued innovation that supports  research globally!    Continued ISO 90001 and 13485 Certification  NimaGen proudly continues to maintain its ISO 90001 and ISO 13485 certification. ISO 90001 is a globally recognized standard for quality management that ensures high-level customer service, ongoing optimization and continued process improvement. ISO 13485 is an international standard that specifies strict requirements for quality and competence in a medical device organization, including biotechnology companies, thus ensuring that we are providing high-quality reagents and products.  Congress and Event Attendance:  NimaGen attended several events around the world in 2023! Some conferences were local to the Netherlands such as Dutch Society of Medical Microbiology and the Royal Dutch Society of Microbiology (KNVM NVMM)  or our very own NimaGen Distributor Meeting here in Nijmegen! Other conferences were  within Europe, such as European Society of Clinical Microbiology and Infectious Diseases (ECCMID) in Denmark and the European Society of Human Genetics (ESHG) in Scotland. We also attended many international ones such as International Association of Forensic Sciences (IAFS) in Australia and the International Symposium on Human Identification (ISHI) for Forensics and the American Society for Human Genetics (ASHG) in the U.S.A!  Local: KNVM NVMM 2023 – Arnhem, The Netherlands    NimaGen Distributor Meeting 2023 - Creating Shared Value with our Partners  Europe: ECCMID 2023 – Copenhagen, Denmark   ESHG 2023 – Edinburgh, Scotland    International: IAFS 2023 – Sydney, Australia         ISHI 2023 – Denver, CO USA ASHG 2023 – Washington, D.C. USA             
News | 12.12.23

PT. Indolab Utama (Indonesia): Welcome to the NimaGen Distributor Network!

NimaGen is happy to announce the collaboration with @PT. Indolab Utama. Indolab Utama focusses on Research Institutes, Food and Beverage Industry, Pharmaceutical Industry, Hospital and Universities in the Indonesian market. They have supported several NimaGen users for some time already and we have now strengthened the partnership with an official distributorship. We look forward to working with Indolab Utama to support the customers in Indonesia better. 
News | 07.12.23

Dutscher (France): Welcome to the NimaGen Distributor Network!

NimaGen is happy to announce a new partnership with Dominique Dutscher SAS. Dutscher will be representing the NimaGen brands in France and support our customers in this region. The company was founded in 1982 and is now one of the largest distribution companies in Europe. They value service, quality, responsiveness and customization as well as a willingness to support customers in all their projects.  We look forward to working with Dutscher to increase the level of service for our French customers!