EasySeq™ 16S Bacterial Identification Library Prep Kit, 2 pools/sample, incl. PCR Master Mix, 96 rxn

SKU: RC-16S096
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EasySeq™ 16S Bacterial Identification
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Product Information

16S rRNA V1-V6 and V9 for Bacterial Identification

Intended use

All species of archaea and bacteria have a 16S gene that codes for the small subunit of the ribosomes. This gene has highly variable regions (V1 to V9) used for taxonomical classifications. The gene also has conserved regions that can be used as targets for primers to amplify the highly variable regions.


Through introduction of Next-generation Sequencing (NGS), the 16S rRNA method is now also widely used to identify one or multiple bacterial species in a single sample.


EasySeq™ 16S Bacterial Identification Library Prep Kit, powered by Reverse Complement PCR (RC-PCR), provides a highly sensitive, cost-effective way to identify bacterial species in culture positive and culture-negative samples through analysis of hypervariable regions V1-V6 and V9. It can be used to decipher polymicrobial samples where traditional Sanger sequencing fails. Furthermore, resources and time are saved because the kit does not require culture and can be used with DNA extracted from many different sample types. 


The multiplex RC-PCR generates six amplicons (V1-2, V3, V4, V5, V6 and V9) that can be sequenced on Illumina® platforms iSeq-100, MiniSeq, MiSeq, NextSeq 500/550 and NextSeq 1000/2000. Depending on the platform, up to 768 samples can be multiplexed.


Please note: EasySeq™ 16S Bacterial Identification Library Prep Kit is designed to sequence the hypervariable regions V1-V6 and V9 in a multiplex reaction using two different probe panels. This requires two Unique Dual Index Plates for associated use. 


The EasySeq™ 16S Bacterial Identification Library Prep Kit allows direct amplification of DNA from clinical materials. The kit can also be used with DNA extracted from pure cultures.

Premium features
Premium features

Cost-efficient Workflow - Confidence in Test Results - Choice in Sequencing Platform and Workload

One closed-tube, single reaction workflow with simultaneous indexing and target amplification reduce hands-on time, pipetting error and minimizes risk of sample contamination
Pre-spotted and breakable Unique Dual Index plates ensure accuracy, match your sample workload and ensure optimal usage, minimizing waste
Single tube sample pooling for library clean-up significantly reduces usage of required magnetic beads and consumables
Unique RC-PCR kinetics promote high-target specificity, sensitivity and coverage uniformity (optimized read depth balance) from low DNA input
Well-balanced read distributions and use of phase-shifted primers reduces PhiX spike-in, maximizing sequencing instrument flow cell capacity
Compatible with various Illumina® platforms and liquid handling systems for medium to high-throughput workflows


''RC-PCR is an ideal compromise between the standard one-fragment approach and metagenomics sequencing and may guide future environmental sequencing studies, in which bacterial diversity is a central subject.'' 


Read more:

- Leontidou et al. (2023)
News | 08.01.24

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