EasySeq™ 16S rRNA V1-6 and V9 Selective Bacterial ID Sequencing Kit, 96 rxn

SKU: RC-16S096
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EasySeq™ 16S rRNA V1-6 and V9 Bacterial ID Sequencing Kit, 96 rxn
Product Information

16S rRNA V1-V6 and V9 library prep for selective bacterial identification

16S rRNA V1-V6 and V9

All bacteria and archaea contain a ribosomal subunit known as 16S that is around 1,500 nucleotides long and contains nine hypervariable regions (V1-V9) spread out among highly conserved regions that are genus- or speciesspecific. Sequencing of the 16S ribosomal RNA (rRNA) gene, an established genetic marker, is the gold standard for the analysis of bacterial samples for identification and classification of both pure cultures and mixed sample analysis. Through introduction of Next-generation Sequencing (NGS), the 16S rRNA method is now also widely used to deconvolute complex microbial communities, such
as human gut microbiomes.


The V1-V9 region is better able to distinguish bacterial species than other sub-regions. EasySeq™ 16S rRNA V1-V6 and V9 Bacterial ID provides the complete analysis of the variable regions V1-6 and V9 of the bacterial 16S rRNA gene. This kit supports an NGS driven bacterial identification strategy for infectious disease, contamination investigation and root cause analysis testing from clinical samples. 

Kit and Platform Compatibility

Please note: EasySeq™ 16S rRNA V1-6 and V9 Bacterial ID NGS Library Prep Kit is designed to sequence the hypervariable regions V1-V6 and V9 in a multiplex reaction using two different probe panels. This requires two Unique Dual Index Plates for associated use.


The EasySeq™ 16S rRNA V1-6 and V9 Bacterial ID Sequencing Kit is used with DNA extracted from pure cultures and also allow direct amplification of DNA from clinical materials.


Compatible with Illumina® MiSeq and NextSeq 1000/2000 (2x250bp) runs, both suitable for 16S metagenomic sequencing.


Targeting the 16S rRNA Gene by Reverse Complement PCR Next-Generation Sequencing: Specific and Sensitive Detection and Identification of Microbes Directly in Clinical Samples

''Here, we demonstrate the clinical utility of bacterial identification in clinical samples by a novel method called 16S RC-PCR. Using 16S RC-PCR, we reveal a significant increase in the number of clinical samples in which a potentially clinically relevant pathogen is identified compared to the commonly used 16S Sanger method. Moreover, RC-PCR allows automation and is well suited for implementation in a diagnostic laboratory. In conclusion, the implementation of this method as a diagnostic tool is expected to result in an increased number of diagnosed bacterial infections, and in combination with adequate treatment, this could improve clinical outcomes for patients.''

Read more here or download PDF version below

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