EasySeq™ 16S Microbiome Kit 96 rxn

The EasySeq™ 16S Microbiome Library Prep Kit enables cost-effective microbiome profiling through Next-Generation Sequencing (NGS). Using Reverse Complement PCR (RC-PCR), this kit provides a streamlined, single-tube workflow for amplifying the hypervariable V3-V4 regions of the 16S rRNA gene. These regions offer high nucleotide heterogeneity, allowing for accurate taxonomic classification and microbial diversity analysis. The kit is designed for direct DNA amplification from various sample types, including environmental, feed, food, human, and animal-derived samples, without requiring bacterial culture.   The workflow integrates indexing and target amplification in one closed-tube reaction, reducing hands-on time and contamination risk. The pre-spotted, breakable Unique Dual Index plates ensure sample accuracy and minimize waste. The single-tube sample pooling simplifies library clean-up, optimizing the use of magnetic beads and consumables.   Compatibility•    Compatible with Illumina® MiSeq and NextSeq 1000/2000 platforms•    Supports multiplexing of up to 768 samples   Kit Components•    PCR Master Mix•    Unique Dual Index Plate (required for sequencing)   Benefits ✔ Cost-effective, single-tube workflow ✔ High sensitivity for low-abundance bacteria ✔ Reduced pipetting errors and contamination risks ✔ Optimized read depth balance and sequencing efficiency ✔ Compatible with liquid handling systems for high-throughput workflows