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The Orange-500 DNA Size Standard is a fluorescent labeled ultra-accurate internal size standard for the reproducible sizing of fragment analysis peaks during capillary electrophoresis and precise DNA fragment size comparisons between capillary electrophoresis runs. This DNA Size Standard contains 16 Orange dye-labeled, single-stranded fragments in the 35-500 nucleotides range with respective basepair lengths of 35, 50, 75, 100, 139, 150, 160, 200, 250, 300, 340, 350, 400, 450, 490 and 500 bp. Each of the DNA fragments is labeled with a LIZ® compatible fluorophore resulting in sharp orange peaks under denaturing conditions. The Orange-500 DNA Size Standard is for use with five-dye or six-dye spectral calibration Genetic Analyzers, such as 3130, 3500 and 3730 and SeqStudio™.
LIZ and SeqStudio are trademarks of Thermo Fisher Scientific.
The Orange-600 DNA Size Standard contains 22 Orange dye-labeled, single-stranded fragments in the 45-595 nucleotides range with respective basepair lengths of 45, 72, 98, 128, 147, 167, 197, 227, 247, 267, 297, 327, 347, 367, 397, 427, 445, 465, 495, 525, 545, 565 and 595 bp. Each of the DNA fragments is labeled with a LIZ® compatible fluorophore resulting in sharp orange peaks under denaturing conditions.
Please note: Orange-600 DNA Size Standard contains 22 DNA size fragments (45 - 595 nucleotide range) versus 36 size fragments present in GeneScan™ 600 LIZ® Dye Size Standards (20–600 nucleotide range).
GeneScan is a trademark of Thermo Fisher Scientific.
The Red-500 DNA Size Standard is a fluorescent labeled ultra-accurate internal size standard for the reproducible sizing of fragment analysis peaks during capillary electrophoresis and precise DNA fragment size comparisons between capillary electrophoresis runs. This DNA Size Standard contains 16 Red dye-labeled, single-stranded fragments in the 35-500 nucleotides range with respective basepair lengths of 35, 50, 75, 100, 139, 150, 160, 200, 250, 300, 340, 350, 400, 450, 490 and 500 bp. Each of the DNA fragments is labeled with ROX® fluorophore resulting in sharp red peaks under denaturing conditions. The Red-500 DNA Size Standard is for use with five-dye or six-dye spectral calibration Genetic Analyzers, such as 3130, 3500 and 3730 and SeqStudio™.
ROX and SeqStudio are trademarks of Thermo Fisher Scientific.
The Red-600 DNA Size Standard contains 22 Red dye-labeled, single-stranded fragments in the 45-595 nucleotides range with respective basepair lengths of 45, 72, 98, 128, 147, 167, 197, 227, 247, 267, 297, 327, 347, 367, 397, 427, 445, 465, 495, 525, 545, 565 and 595 bp. Each of the DNA fragments is labeled with ROX® fluorophore resulting in sharp red peaks under denaturing conditions.
Please note: Red-600 DNA Size Standard contains 22 DNA size fragments (45 - 595 nucleotide range) versus 36 size fragments present in GeneScan™ 600 ROX® Dye Size Standards (20–600 nucleotide range).
GeneScan is a trademark of Thermo Fisher Scientific.
Seq-DI™ Formamide v2 is an ultra-pure highly deionized formamide used to resuspend sequencing samples, including DNA Size Standards, before electrokinetic injection on capillary electrophoresis systems. It is commonly used in fragment analysis. Seq-DI™ Formamide provides ready-for-use injection on 3130, 3500 and 3730 Series Genetic Analyzers and SeqStudio™ Flex, without any requirement for changes in protocol, conditions or spectral calibrations. The proven economical but high-quality alternative to Hi-Di™.
SeqStudio and Hi-Di are trademarks of Thermo Fisher Scientific.
NimaPrime™ Conditioning Reagent is used to prime the polymer pump of Genetic Anayzers, as well as wash the polymer pump and channels when switching between polymer types or performing routine maintenance. The RFID labeled pouche is compatible with all Data Analysis Software versions of 3500 Series and SeqStudio™ Flex. NimaPrime™ Conditioning Reagent is a drop-in replacement for Applied Biosystems™ Conditioning Reagent for 3500/SeqStudio™ Flex.
Applied Biosystems and SeqStudio are trademarks of Thermo Fisher Scientific.
Capillary array as a carrier for polymers (e.g. POP™ and NimaPOP™ separation media) is critical for peak separation in Sanger Sequencing and Fragment Analysis. At the end of the array life, it will not generate well-separated peaks. ReCAP™ Capillary Array Regeneration Kit for 3130 Series Genetic Analyzers has been designed for in-line cleaning of the capillary array, aimed at rejuvenating failing arrays to like new, maintaining optimal performance and improve operational life span. ReCAP™ Capillary Array Regeneration Kit contains 4 different buffer solutions, to be applied sequentially to the pump blocks and capillaries via the instrument’s inbuilt Water Wash Wizard protocol.
ReCAP™ Capillary Array Regeneration Kit for 3730 Series Genetic Analyzers is used to perform a complete rejuvenation and revitalization of old and failing capillary arrays. Particularly those arrays that start to lose resolution, or show smears or tails in the electropherogram. Additionally, it cleans the pump blocks of the machine, preventing or eliminating yellow or red haze. Regeneration extends the lifespan of the array, maintaining optimal performance, avoiding the cost of unnecessary early array replacement. ReCAP™ requires no disassembling or reassembling of the array. There is no need for spectral and spatial calibration after array cleaning.
NimaPOP™ comprises a range of polymers for performing Fragment Analysis of DNA using Capillary Electrophoresis (CE), combined with NimaPOP™ running buffer.
In combination with Seq-DI™ Formamide and DNA Size Standards the ideal solution for separating DNA fragments of a known size range at a desired resolution and run time, for results and data you can trust.