In clinical genetics and high-throughput sequencing environments, human sample identification is not an add-on; it is the foundation of data integrity. As laboratories scale WES and WGS workflows, identification must operate seamlessly within existing processes, without introducing additional complexity or risk. Integrated identification offers a more streamlined approach for laboratories seeking operational efficiency and confidence in data integrity.
Traditional sample tracking methods rely on separate handling steps and verification layers. While effective, they add operational burden and increase workflow complexity. Integrated, single-reaction identification embeds verification directly into library preparation rather than managing it as a separate workflow layer.
NimaGen's RC-PCR technology does something genuinely different. It collapses what has traditionally been a multi-step, error-prone library prep process into a single closed-tube reaction.
Timothy Kupferschmid - Sr. Director Genetic Identity - Promega Group
One tube, single reaction NGS library prep for short read sequencers
EasySeq™ Human DNA Sample Identification enables secure sample verification within the existing NGS workflow. Enabled by Reverse Complement PCR technology, amplification, indexing and library preparation are combined within one streamlined reaction. Sample identification becomes embedded in the sequencing workflow rather than managed as a separate control layer. This supports robust and traceable performance while maintaining the simplicity modern NGS laboratories expect.
Integrated sample identification and data confirmation workflow.
EasySeq™ Human SID workflow overview
Traditional library preparation separates amplification, adapter ligation, and indexing into multiple handling steps. Each step introduces complexity and risk.
Single-reaction library preparation integrates amplification, indexing and adapter addition within one closed-tube workflow. One PCR reaction creates a complete library, while subsequent pooling of 96 samples for cleanup cuts hands-on time by 50%.
"The end product is easy to use, very flexible, highly scalable and provides reliable genetic profiles to ensure sample identity. As a cost-effective solution with minimal hands-on time, the kit could be easily integrated into our existing NGS workflow."
MVZ Labor Krone, Bad Salzuflen, Germany
“Even though we pool 96 samples without individual QC, the balance after sequencing is surprisingly good. A titration experiment confirmed that the protocol remains robust at reduced input. The uniformity across samples is excellent.”
A. Verfaillie - UZ Leuven, Belgium
The advantages of our patented one-tube workflow:
The real test of any Sample Identification system is how it performs across your actual workflows, from routine WES/WGS runs to challenging sample types that push the limits. EasySeq™ has been validated across the scenarios labs encounter every day.
For laboratories where chain of custody must extend beyond extracted DNA, EasySeq™ Blood Direct verifies sample identity from the original blood input. By integrating the isolation step into the identification workflow, potential blind spots are reduced without adding procedural burden.
Human sample identification integrates seamlessly into existing WES and WGS workflows and is validated across commonly used sequencing platforms.
All detailed specifications including coverage ranges, read depth distribution, panel composition and validation data are available in a separate technical document.
Integrated, single-reaction identification reduces complexity while strengthening data integrity. See how it performs in your own laboratory environment.
From WGS and WES to Blood Direct and targeted panels, we provide a tailored test setup based on your application and sequencing platform. After submission, our team will contact you to align on your requirements.
