Product

EasySeq™ Human DNA Sample Identification Kit - Blood Direct, 1 pool/sample, includes PCR Master Mix, 96 rxn

SKU: RC-BDSID096
Variants
Required products
Automatically added to cart
Human DNA Sample Identification
Variants
Required products
Automatically added to cart
Product Information

Track Sample Identity, Safeguard WES/WGS Data Integrity and Validity 

Intended use

EasySeq™ Human DNA Sample Identification Blood Direct Kit, powered by patented Reverse Complement PCR (RC-PCR) technology, facilitates an effective end-to-end identification and tracking of independent samples in a single targeted sequencing assay that is purpose-designed for fast and efficient lab processing. EasySeq™ Human DNA Sample Identification Blood Direct Kit offers validation of sample identity from the very first step of the WES/WGS workflow, including DNA isolation: RC-PCR can be performed directly on the blood sample. 

 

EasySeq™ Human DNA Sample Identification Blood Direct Kit is complementary to both Whole Exome and Whole Genome Sequencing Sample workflows. Sample IDs are matched with WES/WGS data and, as a result, data integrity and validity can be confirmed.

 

The EasySeq™ Human DNA Sample Identification Blood Direct Kit offers a one-tube single-reaction multiplex assay, comprising an optimized panel of 40 exonic targets, the genotypic profile which can be utilized to extract intrinsic identifiers from the human exome and genome: 37 exonic single nucleotide polymorphisms (SNPs) with high minor allele frequency (MAF), and Amelogenin X - Y and TXLNGY for gender determination. This panel is identical to EasySeq™ Human DNA Sample Identification Kit (RC-SID096).

Compatibility

The multiplex RC-PCR generates 40 amplicons that can be sequenced on Illumina® benchtop platforms iSeq, MiniSeq, MiSeq, NextSeq and production-scale sequencers HiSeq and NovaSeq. 

 

Please note: EasySeq™ Human DNA Sample Identification Blood Direct Kit is designed to sequence the set of 40 exonic targets using a single probe pool reaction. This requires one Unique Dual Index Plate for associated use.

 

The EasySeq™ Human DNA Sample Identification Blood Direct Kit allows DNA amplification directly from human blood samples, i.e. fresh or frozen blood, or dry bloodspots.

 

Are you currently using:

- Applied Biosystems QuantStudio™ OpenArray™

- Twist Human Sample ID Kit

- Agena Bioscience iPlex® MassARRAY®

- Homebrew Sample Tracking Method

and interested in running an evaluation of the EasySeq™ Human DNA Sample Identification Blood Direct Kit? Please send us your request by selecting the 'Contact' button.

EasySeq™ Human DNA Sample Identification Blood Direct Kit Workflow
Premium features
Downloads
Premium features

Confidence in test results - Cost-efficient workflow - Choice in workload and platform - Complementary to both WES and WGS workflows

One closed-tube, single reaction workflow with simultaneous indexing and target amplification reduces labor time
Single tube sample pooling for library clean-up significantly reduces usage of magnetic beads and consumables
MAF SNPs selected in combination with RC-PCR kinetics provide high discrimination power; UDIs of 10 bp increase discriminatory power and help to prevent index hopping
Minimizing pipetting error and preventing sample swaps: correct data interpretation and the right critical samples are matched
Well-balanced read distributions maximize sequencing instrument flow cell capacity
Complementary to both Whole Exome and Whole Genome Sequencing workflows on a variety of Illumina® platforms
Blood Direct Kit: include the DNA isolation step in the validation process, to exclude possible sample swabs or contamination