EasySeq™ Human DNA Sample ID Kit 96 rxn - Hereditary Cancer
Description
The EasySeq™ Human DNA Sample Identification Hereditary Cancer Kit is designed to safeguard sample identity and associated WES/WGS or panel sequencing data integrity within existing NGS workflows (RUO).
The kit is a multiplex NGS library preparation solution for genotyping an optimized panel of 32 exonic SNPs with a high Minor Allele Frequency (MAF) in human genes involved with hereditary cancer. This SNP profile enables verification of sequencing data integrity by matching intrinsic identifiers derived from hereditary cancer panel sequencing data, generating Illumina®-compatible libraries.
The workflow enables one-tube, single reaction NGS library preparation, combining target amplification, indexing and adapter addition in a single reaction to reduce handling steps and decrease the risk of PCR contamination and sample mix-ups. This streamlined workflow is enabled by Reverse Complement PCR technology, which supports multiplex amplification and library construction within the same reaction.
| EasySeq™ Human DNA Sample Identification Hereditary Cancer Targets | |||
|---|---|---|---|
| rs3219489 | rs1799977 | rs2228230 | rs1801406 |
| AMEL-X | AMEL-Y | rs1042522 | rs799917 |
| rs357564 | rs2071313 | rs1800860 | rs26279 |
| rs1800392 | rs7862221 | rs1126417 | rs1800935 |
| rs17807673 | rs13712 | rs4986764 | rs13054014 |
| rs866006 | rs2736098 | rs3744093 | rs11528010 |
| rs2066827 | rs3852673 | rs1801552 | rs1126497 |
| rs41737 | rs7935 | rs709816 | rs8556 |
Compatibility
• Illumina® sequencers: iSeq, MiniSeq, MiSeq, NextSeq, NovaSeq
Kit Components
• EasySeq™ Human Sample ID Hereditary Cancer Probe Panel
• Blood Direct HiFi Master Mix (Hot Start HiFi)
• Probe Dilution Buffer Enhanced
Note: This item requires (a) dedicated Index Plate(s).
Once you’ve added this RC-PCR kit to your cart, you'll be prompted to select the appropriate Unique Dual Index Primer Plate(s).
Benefits
✓ 32 exonic SNPs with a high Minor Allele Frequency (MAF)
✓ Direct DNA amplification from blood samples
✓ Detects sample mix-ups and pipetting errors
✓ Single-tube reaction for simplified and safe workflow
✓ Compatible with multiple short read sequencers
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This product is for research use only. Not for use in diagnostic procedures.