Product

D-Pure™ DyeTerminator Cleanup Kit, Magnetic Beads, 500 mL

SKU: DP-500
Variants
DP-500
Variants
Product Information

D-Pure™ Magnetic Bead-based Purification of Cycle Sequencing Extension Products

Intended use

D-Pure™ Dye Terminator Cleanup efficiently removes unincorporated dye terminators and salt-ions from extension products cycle sequenced with BrilliantDye™ or BigDye™ chemistries. Magnetic clean-up is completed directly on the cycle sequencing plate and can be processed both manually and automated.

 

D-Pure™ DyeTerminator Cleanup Kit is the magnetic bead-based alternative to iX-Pure™ DyeTerminator or BigDye XTerminator™ and the high-quality alternative for CleanSEQ DyeTerminator Removal Kit. D-Pure™ is conveniently available in 5 mL, 50 mL and 500 mL format.

Compatibility

D-Pure™ DyeTerminator Cleanup kits are widely adopted as proven, high-quality reagents for laboratories using 3130, 3500/SeqStudio™ Flex or 3730 Series Genetic Analyzers, and are compatible with BigDye XTerminator™ run modules. 

 

Purified dye-labeled extension products can be loaded directly on the Genetic Analyzers without the need for resuspension. D-Pure™ DyeTerminator Cleanup can be optimally used in conjunction with Alpaqua® magnet plates, e.g. the Magnum FLX® or 96S Super Magnet plates.

 

BigDye, BigDye XTerminator and SeqStudio are trademarks of Thermo Fisher Scientific. CleanSeq is a trademark of Beckman Coulter.

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D-Pure™ purified sequencing reactions result in clean chromatograms, very high signal-to-noise (S/N) ratios, high quality scores and long read lengths, with 100% removal of any DyeTerminator blobs.

D-Pure™ does not involve any centrifugation or filtration steps, allowing for easy manual processing, and full automation using liquid handlers.

Effective removal of cycle sequencing reaction contaminants
Cost-efficient product, with straightforward protocol
Ideal for high-throughput applications
Value for many versus CleanSEQ and BigDye XTerminator™
Reference

“Cost-performance relation is significantly better than that of the other vendors”

The Human Genetics Department decided to use NimaGen´s D-Pure™ DyeTerminator kit on the Hamilton Microlab® STARlet for its future sequencing projects, since its cost-perfomance relation is significantly better than that of the other vendors, with no compromise in data quality and recovery.

Department of Human Genetics, Radboud University Medical Center, Nijmegen, The Netherlands
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