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D-Pure™ Dye Terminator Cleanup efficiently removes unincorporated dye terminators and salt-ions from extension products cycle sequenced with BrilliantDye™ and BigDye™ chemistries. Magnetic clean-up is completed directly on the cycle sequencing plate and can be processed both manually and automated. D-Pure™ is conveniently available in 5 mL, 50 mL and 500 mL format.
D-Pure™ DyeTerminator Cleanup kits are widely adopted as proven, high-quality reagents for laboratories using 310, 3100, 3130, 3500, 3730 Series Genetic Analyzers and SeqStudio™, and are compatible with BigDye™ and BrilliantDye™ Cycle Sequencing (v1.1 and v3.1) Kits. Purified dye-labeled extension products can be loaded directly on the Genetic Analyzers without the need for resuspension. D-Pure™ DyeTerminator Cleanup can be used in conjunction with Alpaqua® magnet plates, e.g. the 96R Ring Magnet Plate.
BigDye and SeqStudio are trademarks of Applied Biosystems, LLC
D-Pure™ purified sequencing reactions result in clean chromatograms, very high signal-to-noise (S/N) ratios, high quality scores and long read lengths, with 100% removal of any DyeTerminator blobs.
D-Pure™ does not involve any centrifugation or filtration steps, allowing for easy manual processing, and full automation using liquid handlers.
The Human Genetics Department decided to use NimaGen´s D-Pure™ DyeTerminator kit on the Hamilton Microlab® STARlet in its future sequencing projectsm since its cost-perfomance relation is significantly better than that of the other vendors, with no compromise in data quality and recovery.