Product

D-Pure™ DyeTerminator Cleanup Kit, Magnetic Beads, 5 mL

SKU: DP-005
Variants
DP-005
Variants
Product Information

D-Pure™ Magnetic Bead-based Purification of Cycle Sequencing Extension Products

Intended use

D-Pure™ Dye Terminator Cleanup efficiently removes unincorporated dye terminators and salt-ions from extension products cycle sequenced with BrilliantDye™ or BigDye™ chemistries. Magnetic clean-up is completed directly on the cycle sequencing plate and can be processed both manually and automated.

 

D-Pure™ DyeTerminator Cleanup Kit is the magnetic bead-based alternative to iX-Pure™ DyeTerminator or BigDye XTerminator™ and the high-quality alternative for CleanSEQ DyeTerminator Removal Kit. D-Pure™ is conveniently available in 5 mL, 50 mL and 500 mL format.

Compatibility

D-Pure™ DyeTerminator Cleanup kits are widely adopted as proven, high-quality reagents for laboratories using 3130, 3500/SeqStudio™ Flex or 3730 Series Genetic Analyzers, and are compatible with BigDye XTerminator™ run modules. 

 

Purified dye-labeled extension products can be loaded directly on the Genetic Analyzers without the need for resuspension. D-Pure™ DyeTerminator Cleanup can be optimally used in conjunction with Alpaqua® magnet plates, e.g. the Magnum FLX® or 96S Super Magnet plates.

 

BigDye, BigDye XTerminator and SeqStudio are trademarks of Thermo Fisher Scientific. CleanSeq is a trademark of Beckman Coulter.

Premium features
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Premium features

D-Pure™ purified sequencing reactions result in clean chromatograms, very high signal-to-noise (S/N) ratios, high quality scores and long read lengths, with 100% removal of any DyeTerminator blobs.

D-Pure™ does not involve any centrifugation or filtration steps, allowing for easy manual processing, and full automation using liquid handlers.

Effective removal of cycle sequencing reaction contaminants
Cost-efficient product, with straightforward protocol
Ideal for high-throughput applications
Value for many versus CleanSEQ and BigDye XTerminator™
Reference

“Cost-performance relation is significantly better than that of the other vendors”

The Human Genetics Department decided to use NimaGen´s D-Pure™ DyeTerminator kit on the Hamilton Microlab® STARlet in its future sequencing projectsm since its cost-perfomance relation is significantly better than that of the other vendors, with no compromise in data quality and recovery.

Department of Human Genetics, Radboud University Medical Center, Nijmegen, The Netherlands
Product news

Latest news

News | 08.01.24

NimaGen's 2023 Highlights ReCAPPed!

NimaGen had a great 2023 and were looking forward to an even better 2024!    NimaGen’s 2023 ReCAPPed™ (Yes, this a pun on our product name):  Our New Building and Facilities:  We kicked off 2023 by moving into our new building with state-of-the-art laboratory and production spaces, as well as comfortable office and meeting spaces! Our NimaGen Grand Opening party in May was an exciting event celebrated by all to commemorate such a momentous occasion; it was also attended by our local major of Nijmegen!            Our New Reverse Complement PCR Sequencing Kits:  From there, we spent the year developing several innovative and exciting new Reverse-Complement PCR sequencing kits for our application areas in Forensics, Microbiology and Infectious Disease and Human Genetics!      Distributors, Partnerships and Collaborations In 2023, NimaGen gained 15 partnerships around the world that help support our expanding product portfolio! From the far reaches of Barcode Biosciences in India to our neighboring country France supported by Dominique Dutscher SAS, as well as strategic collaborations with partnerships in North American with NicheVision for our Forensics portfolio, we desire to help our customers in every cornerstone of the world. Together, we will continue creating shared value!    Customer Experience Survey Rating At the end of 2023, active NimaGen customers received a customer experience survey with an average score of >8/10!. NimaGen responsiveness, solutions focus, personal interaction and product value were highly rated by both customers and distributors. We are grateful for the feedback we received and will continue to strive to continue improve our customer service, quality products and continued innovation that supports  research globally!    Continued ISO 90001 and 13485 Certification  NimaGen proudly continues to maintain its ISO 90001 and ISO 13485 certification. ISO 90001 is a globally recognized standard for quality management that ensures high-level customer service, ongoing optimization and continued process improvement. ISO 13485 is an international standard that specifies strict requirements for quality and competence in a medical device organization, including biotechnology companies, thus ensuring that we are providing high-quality reagents and products.  Congress and Event Attendance:  NimaGen attended several events around the world in 2023! Some conferences were local to the Netherlands such as Dutch Society of Medical Microbiology and the Royal Dutch Society of Microbiology (KNVM NVMM)  or our very own NimaGen Distributor Meeting here in Nijmegen! Other conferences were  within Europe, such as European Society of Clinical Microbiology and Infectious Diseases (ECCMID) in Denmark and the European Society of Human Genetics (ESHG) in Scotland. We also attended many international ones such as International Association of Forensic Sciences (IAFS) in Australia and the International Symposium on Human Identification (ISHI) for Forensics and the American Society for Human Genetics (ASHG) in the U.S.A!  Local: KNVM NVMM 2023 – Arnhem, The Netherlands    NimaGen Distributor Meeting 2023 - Creating Shared Value with our Partners  Europe: ECCMID 2023 – Copenhagen, Denmark   ESHG 2023 – Edinburgh, Scotland    International: IAFS 2023 – Sydney, Australia         ISHI 2023 – Denver, CO USA ASHG 2023 – Washington, D.C. USA             
News | 12.12.23

PT. Indolab Utama (Indonesia): Welcome to the NimaGen Distributor Network!

NimaGen is happy to announce the collaboration with @PT. Indolab Utama. Indolab Utama focusses on Research Institutes, Food and Beverage Industry, Pharmaceutical Industry, Hospital and Universities in the Indonesian market. They have supported several NimaGen users for some time already and we have now strengthened the partnership with an official distributorship. We look forward to working with Indolab Utama to support the customers in Indonesia better. 
News | 07.12.23

Dutscher (France): Welcome to the NimaGen Distributor Network!

NimaGen is happy to announce a new partnership with Dominique Dutscher SAS. Dutscher will be representing the NimaGen brands in France and support our customers in this region. The company was founded in 1982 and is now one of the largest distribution companies in Europe. They value service, quality, responsiveness and customization as well as a willingness to support customers in all their projects.  We look forward to working with Dutscher to increase the level of service for our French customers!