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Reverse Complement (RC-PCR) based MPS library prep solution for Y-STR haplotyping, to characterise paternal lineages of unknown male trace donors.
RC-PCR provides multiplex targeted amplification, sequencing indexing and adaptor addition occuring simultaneously in a closed one-tube single reaction.
All amplicons are designed to meet the shortest possible fragment lengths. The multiplex RC-PCR generates amplicons that can be sequenced on Illumina® platforms MiSeq, (Qiagen/Verogen) MiSeq FGx, and NextSeq 1000. Depending on the platform, up to 768 samples can be multiplexed, with 2x 10 bp Unique Dual Index reads.
IDseek® Kits have been tested with Tannic Acid and Humic Acid. RC-PCR technology allows the reliable analysis of highly degraded samples frequently found in forensic casework.
IDseek® comprises a complete solution of reagents and consumables required for generating sequence-ready MPS libraries for Forensic applications: from indexing to amplification.
IDseek® reagents and consumables required for 96 reactions:
- RC-PCR Probe Mix
- RC-PCR HiFi 2x Hotstart PCR Mastermix
- Dehydrated Pre-spotted Unique Index Primer Plate
- Probe Dilution Buffer
- AmpliClean™ Cleanup, Magnetic Beads
A magnet compatible with 1.5 mL/2.0 mL centrifuge tubes is recommended for use with the AmpliClean™ Magnetic Bead Cleanup.
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