IDseek® CombiSTR™ Plus Autosomal and Y-chromosomal STR profiling Kit 96 rxn
Description
The IDseek® CombiSTR™ Plus Kit is a Reverse Complement (RC-PCR) based MPS library preparation solution designed for Y-STR haplotyping, enabling the characterization of paternal lineages from unknown male trace donors. This closed one-tube reaction system integrates multiplex targeted amplification, sequencing indexing, and adaptor addition. The method ensures efficient library preparation with minimal handling, reducing contamination risks.
This closed one-tube reaction system supports multiplexing of up to 768 samples with 2x10 bp Unique Dual Index reads and is validated for use with degraded forensic samples containing Tannic Acid and Humic Acid.
A magnet compatible with 1.5 mL/2.0 mL centrifuge tubes is recommended for bead cleanup.
Compatibility
• Compatible with Illumina® MiSeq, (Qiagen/Verogen) MiSeq FGx, and NextSeq 1000.
Kit components
• RC-PCR Probe Mix
• RC-PCR HiFi 2x Hotstart PCR Master Mix
• Dehydrated Pre-spotted Unique Index Primer Plate
• Probe Dilution Buffer
• AmpliClean™ Cleanup, Magnetic Beads
Note: This item requires (a) dedicated Index Plate(s).
Once you’ve added this RC-PCR kit to your cart, you'll be prompted to select the appropriate Unique Dual Index Primer Plate(s).
Benefits
✓ One-tube reaction minimizes handling and contamination risk
✓ Optimized for degraded forensic samples
✓ Compatible with multiple Illumina sequencing platforms
✓ Supports high-throughput multiplexing of up to 768 samples
✓ Includes all reagents for sequence-ready library preparation
This product is for research use only. Not for use in diagnostic procedures.