Nucleic Acid Extraction and Clean-up

ExS-Pure™ Enzymatic PCR Purification Kit removes excess primers and unincorporated nucleotides from the PCR reaction prior to sequencing, as any leftover primers can interfere with the strand-specific cycle sequencing reactions that will follow.  This kit provides a ready-for-use reagent containing a well-balanced mixture of two highly effective enzymes, recombinant hydrolytic and heat-labile enzymes, Exonuclease-I and Shrimp Alkaline Phosphatase. Prior to cycle sequencing, ExS-Pure™ is added directly to the PCR product, and cleanup is performed in a standard thermal cycler within 5 minutes. ExS-Pure™ is the widely adopted drop-in alternative to ExoSAP-IT™. 

ExoSAP-IT is a trademark of Thermo Fisher Scientific.

The AmpliClean™ Clean-up Kit provides highly efficient and cost-effective magnetic bead-based technology for high-throughput purification of PCR products.  AmpliClean™ removes, salts, primers, primer-dimers and dNTPs, leaving ultra-pure DNA of the desired length. It requires no centrifugation or vacuum filtration steps. Purified PCR products can be used directly for Sanger cycle sequencing.  The AmpliClean™ protocol is highly flexible for manual operation and can be easily incorporated in any liquid handling system for automated processing. AmpliClean™ magnetic beads are the widely adopted alternative to AMPure XP beads for purification.

AMPure XP is a trademark of Beckman Coulter.

The iX-Pure™ DyeTerminator Clean-up Kit efficiently removes unincorporated dyeterminators and salt-ions from extension products cycle sequenced with BrilliantDye™ and BigDye™ chemistries. Resin-based clean-up is completed within 40 minutes. Purified dye-labeled extension products can be injected directly into Genetic Analyzers. The iX-Pure™ Dye Terminator Clean-up Kit is the proven drop-in alternative for BigDye XTerminator™.

BigDye and BigDye XTerminator are trademarks of Thermo Fisher Scientific.

The D-Pure™ DyeTerminator Cleanup Kit is the magnetic bead-based alternative to iX-Pure™ DyeTerminator or BigDye XTerminator™ and the high-quality alternative for CleanSEQ DyeTerminator Removal Kit.  Magnetic clean-up is completed directly on the cycle sequencing plate, either manually or automated. Purified products can be loaded directly on Genetic Analyzers without further resuspension. Compatible with BigDye XTerminator™ run modules.

BigDye and BigDye XTerminator are trademarks of Thermo Fisher Scientific. CleanSEQ is a trademark of Beckman Coulter.