PCR Purification Kits
ExS-Pure™ Enzymatic PCR Purification Kit removes excess primers and unincorporated nucleotides from the PCR reaction prior to sequencing, as any leftover primers can interfere with the strand-specific cycle sequencing reactions that will follow. This kit provides a ready-for-use reagent containing a well-balanced mixture of two highly effective enzymes, recombinant hydrolytic and heat-labile enzymes, Exonuclease-I and Shrimp Alkaline Phosphatase. Prior to cycle sequencing, ExS-Pure™ is added directly to the PCR product, and cleanup is performed in a standard thermal cycler within 5 minutes. ExS-Pure™ is the widely adopted drop-in alternative to ExoSAP-IT™.
ExoSAP-IT is a trademark of Thermo Fisher Scientific.
The AmpliClean™ Clean-up Kit provides highly efficient and cost-effective magnetic bead-based technology for high-throughput purification of PCR products. AmpliClean™ removes, salts, primers, primer-dimers and dNTPs, leaving ultra-pure DNA of the desired length. It requires no centrifugation or vacuum filtration steps. Purified PCR products can be used directly for Sanger cycle sequencing. The AmpliClean™ protocol is highly flexible for manual operation and can be easily incorporated in any liquid handling system for automated processing. AmpliClean™ magnetic beads are the widely adopted alternative to AMPure XP beads for purification.
AMPure XP is a trademark of Beckman Coulter.
DyeTerminator Clean-up Kits
The iX-Pure™ DyeTerminator Clean-up Kit efficiently removes unincorporated dyeterminators and salt-ions from extension products cycle sequenced with BrilliantDye™ and BigDye™ chemistries. Resin-based clean-up is completed within 40 minutes. Purified dye-labeled extension products can be injected directly into Genetic Analyzers. The iX-Pure™ Dye Terminator Clean-up Kit is the proven drop-in alternative for BigDye XTerminator™.
BigDye and BigDye XTerminator are trademarks of Thermo Fisher Scientific.
The D-Pure™ DyeTerminator Cleanup Kit is the magnetic bead-based alternative to iX-Pure™ DyeTerminator or BigDye XTerminator™ and the high-quality alternative for CleanSEQ DyeTerminator Removal Kit. Magnetic clean-up is completed directly on the cycle sequencing plate, either manually or automated. Purified products can be loaded directly on Genetic Analyzers without further resuspension. Compatible with BigDye XTerminator™ run modules.
BigDye and BigDye XTerminator are trademarks of Thermo Fisher Scientific. CleanSEQ is a trademark of Beckman Coulter.
Purification and Clean-up Kits
D-Pure™ DyeTerminator Cleanup Kit, Magnetic Beads, 5 mL
D-Pure™ DyeTerminator Cleanup Kit, Magnetic Beads, 50 mL
D-Pure™ DyeTerminator Cleanup Kit, Magnetic Beads, 500 mL
AmpliClean™ Cleanup Kit, Magnetic Beads (5 mL)
AmpliClean™ Cleanup Kit, Magnetic Beads (50 mL)
AmpliClean™ Cleanup Kit, Magnetic Beads (500 mL)
ExS-Pure™ Enzymatic PCR Purification Kit, 100 rxn
ExS-Pure™ Enzymatic PCR Purification Kit, 500 rxn
ExS-Pure™ Enzymatic PCR Purification Kit, 5000 rxn
iX-Pure™ DyeTerminator Cleanup Kit, Resin Based, 100 preps
iX-Pure™ DyeTerminator Cleanup Kit, Resin Based, 1000 preps
iX-Pure™ DyeTerminator Cleanup Kit, Resin Based, 2500 preps
iX-Pure™ DyeTerminator Cleanup Kit, Resin Based, 40000 preps
Alpaqua® Magnet Plates
Alpaqua® is a trusted partner to a variety of industry-leading reagent, accessory and liquid handling providers. Alpaqua® magnets are SBS compliant and are the most widely used magnet plates in automation platforms.
Alpaqua magnet plates are widly adopted in a variety of applications, from nucleic acid extraction from a sample, and from PCR purification to dyeterminator clean-up and NGS library preparation. They are the recommended plates in combination with magnetic bead-based solutions such as AMPure XP, AmpliClean™, CleanSEQ or D-Pure™.