Nucleic Acid Extraction and Clean-up

Following nucleic acid extraction from a sample, an integral part of any DNA sequencing workflow is effectively removing excess primers, unincorporated nucleotides, or salts from the PCR reaction prior to sequencing. Eliminating unincorporated dyeterminators from extension products cycle sequenced with BigDye™ or BrilliantDye™. And effective clean-up and size selection in NGS library prep. Pivotal in generating high-quality experimental results and reliable data.

PCR Purification Kits

ExS-Pure™ Enzymatic PCR Purification Kit

ExS-Pure™ Enzymatic PCR Purification Kit removes excess primers and unincorporated nucleotides from the PCR reaction prior to sequencing, as any leftover primers can interfere with the strand-specific cycle sequencing reactions that will follow.  This kit provides a ready-for-use reagent containing a well-balanced mixture of two highly effective enzymes, recombinant hydrolytic and heat-labile enzymes, Exonuclease-I and Shrimp Alkaline Phosphatase. Prior to cycle sequencing, ExS-Pure™ is added directly to the PCR product, and cleanup is performed in a standard thermal cycler within 5 minutes. ExS-Pure™ is the widely adopted drop-in alternative to ExoSAP-IT™. 

 

ExoSAP-IT is a trademark of Thermo Fisher Scientific.

AmpliClean™ Clean-up Kit, Magnetic Beads

The AmpliClean™ Clean-up Kit provides highly efficient and cost-effective magnetic bead-based technology for high-throughput purification of PCR products.  AmpliClean™ removes, salts, primers, primer-dimers and dNTPs, leaving ultra-pure DNA of the desired length. It requires no centrifugation or vacuum filtration steps. Purified PCR products can be used directly for Sanger cycle sequencing.  The AmpliClean™ protocol is highly flexible for manual operation and can be easily incorporated in any liquid handling system for automated processing. AmpliClean™ magnetic beads are the widely adopted alternative to AMPure XP beads for purification.

 

AMPure XP is a trademark of Beckman Coulter.

DyeTerminator Clean-up Kits

iX-Pure™ DyeTerminator Clean-up Kit

The iX-Pure™ DyeTerminator Clean-up Kit efficiently removes unincorporated dyeterminators and salt-ions from extension products cycle sequenced with BrilliantDye™ and BigDye™ chemistries. Resin-based clean-up is completed within 40 minutes. Purified dye-labeled extension products can be injected directly into Genetic Analyzers. The iX-Pure™ Dye Terminator Clean-up Kit is the proven drop-in alternative for BigDye XTerminator™.

 

BigDye and BigDye XTerminator are trademarks of Thermo Fisher Scientific.

D-Pure™ DyeTerminator Clean-up Kit

The D-Pure™ DyeTerminator Cleanup Kit is the magnetic bead-based alternative to iX-Pure™ DyeTerminator or BigDye XTerminator™ and the high-quality alternative for CleanSEQ DyeTerminator Removal Kit.  Magnetic clean-up is completed directly on the cycle sequencing plate, either manually or automated. Purified products can be loaded directly on Genetic Analyzers without further resuspension. Compatible with BigDye XTerminator™ run modules.

 

BigDye and BigDye XTerminator are trademarks of Thermo Fisher Scientific. CleanSEQ is a trademark of Beckman Coulter.

Also Available

Alpaqua® Magnet Plates

Alpaqua® is a trusted partner to a variety of industry-leading reagent, accessory and liquid handling providers. Alpaqua® magnets are SBS compliant and are the most widely used magnet plates in automation platforms.

Alpaqua magnet plates are widly adopted in a variety of applications, from nucleic acid extraction from a sample, and from PCR purification to dyeterminator clean-up and NGS library preparation. They are the recommended plates in combination with magnetic bead-based solutions such as AMPure XP, AmpliClean™, CleanSEQ or D-Pure™.

View Alpaqua® Magnet Plates