Product

ExS-Pure™ Enzymatic PCR purification kit, 100 rxn

SKU: EXS-100
Variants
EXS-100
Variants
Product Information

Fast & Easy PCR Cleanup

Format

The kit consists of two recombinant hydrolytic enzymes: Exonuclease-I and Shrimp Alkaline Phosphatase. Together they eliminate all unwanted dNTP’s and residual primers from your PCR products, which would interfere with downstream applications.

100% Sample recovery

With ExS-Pure™ there is no need for time-consuming gel, column or magnetic bead purifications. Both short and long stranded PCR products are left completely intact.

Premium features
Downloads
Premium features

DNA sequencing, SNP analysis and many other applications require PCR products which are free of dNTP's and primers. For DNA sequencing, unincorporated primers and dNTP's can lead to high background and miscalling of bases. With Ex's-Pure you can efficiently remove these contaminants and make improvements in read length and base calling.

The straightforward workflow allows the cleanup to be performed in under 10 minutes, including hands-on time.

6 minute protocol
Removes primers and dNTPs from PCR products
Add directly to your PCR product
Easily incorporated in automated workflows
100% sample recovery
Reference

Quality of traces

I've just received the sequencing results and I'm quite impressed by the quality of the traces.

ExS-Pure trail - Norway
Product news

Latest news

News | 30.03.21

Press Release: NimaGen- Expanding Throughput for SARS-CoV-2 Whole Genome Sequencing

Nijmegen, The Netherlands, March 29, 2021   Following the release and success of their EasySeq™ SARS-CoV-2 WGS kit in 2020, NimaGen BV announce the further expansion of their kit range to facilitate higher throughput application of SARS-CoV-2 Whole Genome Sequencing (WGS) with the launch of a 384-sample version kit for Illumina sequencing and the addition of an expanded selection of accompanying Unique Dual Indexes (UDIs) now increased to 1536 UDIs. Rapid and Simplified Library Preparation for viral surveillanceThe recent emergence and spread of new nCoV-2 variants have clearly demonstrated the importance and need for rapid and high throughput screening for SARS-CoV-2 for viral surveillance. Utilising Reverse Complement PCR (RC-PCR), WGS library preparation with the EasySeq™ SARS-CoV-2 kit could not be simpler- with just a single PCR reaction for both Amplicon generation and indexing.Assay set-up is extremely easy and ideally suited for automation, with just 2 dispensing steps of sample and Mastermix to pre-spotted index plates before loading in the Thermocycler. Following the single PCR all samples can immediately be pooled for a single-tube magnetic bead clean-up step and once standard quality control checks have been performed the library is ready for sequencing.This drastically simplified protocol can reduce hands on time for library prep by up to 80% compared with other methods currently being used for the WGS of SARS-CoV-2. Dr Josh Quick from the Institute of Microbiology & Infection, University of Birmingham, UK and who was integrally involved in the creation of the ARTIC Network SARS-CoV-2 sequencing protocol has commented:"Multiplex RC-PCR is one of the solutions emerging from the pandemic that has excited me the most. The approach combines the robustness of amplicon sequencing with the scalability of PCR barcoding in a single PCR step, something I have puzzled over for years. In our testing the sensitivity was comparable to other amplicon based methods but with a greatly streamlined Illumina library preparation workflow and I am optimistic that there is even more performance to come with this approach in future." Simpler and more rapid library prep does allow for more samples to be prepared each day, improving public health authority’s ability to monitor the epidemic and the emergence, and spread of new variants. Sequence every positive COVID-19 Sample?“The addition of a 384 well version of the kit to the range will certainly help our high-throughput customers streamline their library preparation even further” says Joop Theelen, CSO and co-founder of NimaGen “Also, with up to 1536 accompanying UDIs now available, more than 3000 SARS-CoV-2 whole genomes could be sequenced on one Illumina NovaSeq SP dual lane flowcell – bringing the possibility to sequence every positive COVID-19 sample closer than ever before.” Future DevelopmentsNimaGen are also rapidly developing a “one-step” version of the kit that works direct on RNA samples, thus avoiding the need for a separate cDNA synthesis reaction before WGS library preparation.By adapting the RC-PCR method cDNA synthesis, amplification and indexing can be carried out in a single closed tube PCR reaction, further streamlining the complete workflow, and allowing for increased throughput.“The need for a separate cDNA synthesis step is currently a potential major workflow bottleneck for our customers and as with any additional handling step it also adds to the risks around assay and sample security” Walter van der Vliet, NGS Application & Development Scientist at NimaGen says “If we can incorporate the generation of cDNA into the RC-PCR reaction then our customers can go direct from extracted RNA to indexed amplicons in just one PCR step, making the whole workflow faster and more secure and ultimately allowing them to process more samples each day” Early development data looks positive and NimaGen expects to have the product commercially available in spring 2021.Further details available at www.nimagen.com/covid19