BrilliantDye™ Terminator (v3.1) Cycle Sequencing Kit (24 rxn)

SKU: BRD3-024
Product Information

BrilliantDye™ Terminator (v3.1) Cycle Sequencing Kit


BrilliantDye™ Terminator (v3.1) Cycle Sequencing Kit provides the reagents (BrilliantDye Terminator, 5x sequencing buffer, pGEM control, M13 control primer) required for Sanger Sequencing reactions, in a ready for use pre-mixed format. It is optimized to provide a robust and highly flexible chemistry, designed for all kinds of DNA sequencing applications, including de novo and resequencing.


BrilliantDye™ Terminator (v3.1) Cycle Sequencing Kit is conveniently offered in low to medium to high-throughput packaging, ranging from 24, 100, 1,000, and 5,000 to 25,000 reactions respectively.


BrilliantDye™ Terminator Cycle Sequencing kits and associated buffers are widely adopted as proven, high-quality reagents for laboratories using 310, 3100, 3130, 3500, 3730 Series Genetic Analyzers and SeqStudio™.


BrilliantDye™ is developed for performing fluorescence-based cycle sequencing reactions on single-stranded or double-stranded DNA templates, including PCR fragments and plasmids. BrilliantDye™ generates data with uniform peak heights and optimized signal balance to produce long, high-quality reads, as well as highly accurate base assignments.


BrilliantDye™ Terminator v3.1 Cycle Sequencing Kits are a drop-in replacement for BigDye® Terminator v3.1 Cycle Sequencing Kits without having to change protocol, volume, or setting. The products can be used with other products in the Sanger sequencing workflow (e.g. NimaPOP™ or POP™ polymers and buffers) on Applied Biosystems® 3130, 3500, 3730 and SeqStudio™ Flex Genetic Analyzers.


BrilliantDye and NimaPOP are trademarks of NimaGen, B.V. SeqStudio and POP are a trademark of Thermo Fisher Scientific, Inc.

Premium features
Premium features
Based on the proven Sanger Chain Termination method
No need for changes in run protocol, setting or calibration
Available in chemistry versions v1.1, v3.1 and dGTP (v1.1 and v3.1)
Compatible with all Genetic Analyzers

How can we help to find the right answer for your question. If your question is not listed below, please use the contact form.

How can I troubleshoot an issue with Sanger Sequencing?

A control primer (M13) and template (pGEM) are provided with each BrilliantDye™ Terminator Cycle Sequencing Kit. These can be run following the BrilliantDye™ protocol and can help you determine whether failed reactions are caused by poor template quality or sequencing reaction failure.

What is the difference between the different versions of BrilliantDye™?

The BrilliantDye™ Terminator v1.1 Cycle Sequencing Kit is designed for specialty applications that require optimal basecalling adjacent to the primer.

The BrilliantDye™ Terminator v3.1. Cycle Sequencing Kit is the method of choice for longest reads.

For sequencing through G- and GT-rich templates, NimaGen offers the dGTP BrilliantDye™ Terminator v1.1 and v3.1 Cycle Sequencing Kits, optimized for challenging sequence templates.

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