For all your questions about our products you can use search box below. You will be send directly to the product page with the current frequently asked questions.
Information
How do I make changes to an order I’ve already placed?
As soon as you place your order, we work on the dispatch process as quickly as possible, if you decide you want to amend your order please contact us and we will try to accommodate where possible.
When will my order ship out?
Orders have a processing time of 1-2 business days. Once dispatched, an email will be sent containing the tracking number for the parcel.
Which shipping carrier is used?
Depending on the shipping destination, DHL or FedEx is used. If preferred we also have options for UPS and TNT.
What can I do if my order is going to the wrong address?
Please contact us immediately, so we can attempt to contact the courier to return the parcel back to us for a refund.
I would like to pay by Credit Card, is this possible?
Yes, you can follow the normal order process and indicate this in the “Extra information” field. We will be in touch to process the CC payment.
What currencies can I use?
By default payment and invoicing is in Euro (EUR). Upon request we can also process Great British Pound (GBP) and United States Dollar (USD).
How do I return an item?
If you’re returning item(s) to us, please contact us with your name and order number at info@nimagen.com where we will be able to advise you further on our international returns process.
Please make sure temperature sensitive items are stored correctly in the meantime.
What is the maximum Ct value for a positive sample to be able to sequence?
We tested the kit with samples of Ct32 or lower. For samples with Ct <25 we recommend to dilute the cDNA
What can I do to sequence through a hairpin or secondairy structure?
- Use the BrilliantDye dGTP kit for the sequencing reaction. For optimal results it can be mixed with the default BrilliantDye kits to increase read lenghts.
- Add DMSO to a final 5% (v/v) concentration to the sequencing reaction.
- Incubate the reaction at 96C for 10 minutes before cycle sequencing.
What is the difference between the different versions of BrilliantDye™?
The BrilliantDye™ Terminator v1.1 Cycle Sequencing Kit is designed for specialty applications that require optimal basecalling adjacent to the primer.
The BrilliantDye™ Terminator v3.1. Cycle Sequencing Kit is the method of choice for longest reads.
For sequencing through G- and GT-rich templates, NimaGen offers the dGTP BrilliantDye™ Terminator v1.1 and v3.1 Cycle Sequencing Kits, optimized for challenging sequence templates.
I would like to have more information about your products?
We work hard to include as much information about our products in our product descriptions on the product page, but if there’s something more specific you’d like to know please contact us at info@nimagen.com.
What is Reverse Complement PCR (RC-PCR) Technology?
Reverse Complement PCR (RC-PCR) is our patented, IP-protected technology, exclusively licensed to NimaGen B.V. by Salisbury NHS Foundation Trust. It powers our One-tube, Single Reaction NGS Library Prep, integrating amplification, indexing, and adapter ligation in a single closed reaction, unlike traditional PCR methods. Production, distribution, or use of RC-PCR-based methods or kits without written consent is strictly prohibited.
Is the workflow really closed-tube?
Yes. Amplification, indexing and adapter ligation take place in a single, closed reaction, minimizing handling and contamination risk.
Which sequencing platforms are supported?
Libraries are compatible with all major sequencing platforms.
How much hands-on time is required?
Preparing sequencing-ready libraries requires approximately 40 minutes of hands-on time, including pooled cleanup.
How many samples can be multiplexed?
Depending on index availability, up to 384 or more samples can be multiplexed in a single sequencing run.
How sensitive is the method?
The workflow supports reliable library preparation from low DNA input, down to the pg range, with balanced amplification and high specificity.
Do I need specialist training?
No specialist training is required. The workflow is designed to be as straightforward as a standard PCR protocol.
Can libraries be pooled before cleanup?
Yes. Individual reactions can be pooled prior to cleanup, reducing hands-on time and consumable use.
Is automation possible?
Yes. The workflow can be implemented on a variety of liquid handling platforms.
What are custom NGS panels?
NG-Assay-on-Request™ provides fully customized NGS library prep kits for targeted sequencing, designed around your specific genomic targets rather than a generic catalogue selection. Built from scratch, or designed to extend the content of an existing panel. Free project consultation with our NGS experts is included, dedicated to a quick and practical turnaround.
Why might standard NGS panels not meet my specific research needs?
Most commercial panels are built for the broadest possible market, which means they are rarely optimized for any single research context.
Too narrow: Your variants of interest are absent from every available commercial panel, forcing you to accept incomplete coverage or invest months in an in-house design.
Too broad: You pay for sequencing depth across targets that add no value to your study, driving up costs without improving the quality of your results.
Time-consuming: Designing a custom panel in-house requires bioinformatics capacity for in silico probe design, wet-lab time for iterative validation, and expertise most diagnostic or research labs cannot spare, with development timelines that routinely stretch to several months.
Unreliable: Self-designed panels introduce risk at every open-tube step, from probe design failures and uneven GC content to indexing errors and cross-contamination during library preparation.
The kit should fit your science, not the other way around, and that is precisely the problem NG-Assay-on-Request™ was built to solve.
What results can I expect from NG-Assay-on-Request™?
NG-Assay-on-Request™ delivers high on-target performance (≥70% reads), uniform coverage (≥95% within 3-fold of median), and reproducible results, all in a single reaction. Expect faster turnaround, fewer errors, and full traceability for your custom targets.
What applications and biological domains can NG-Assay-on-Request™ support?
NG-Assay-on-Request™ supports a wide range of applications across human, animal, plant, and microbiology domains, including hereditary diseases, cancer, forensic identification, breeding programs, QA/QC, pathogen detection, crop improvement, strain typing, and resistance analysis. If your target database exists, we can design a validated panel around it
How does the NG-Assay-on-Request™ process work?
The NG-Assay-on-Request™ process consists of four steps:
1. Submit your species, genome, targets, platform, and sample volume.
2. Design by our R&D team, ensuring specificity and balance.
3. Validate through wet-lab confirmation of on-target performance and coverage uniformity.
4. Deliver your ready-to-use kit, assembled and shipped.
This tailored process meets the needs of clinical labs, research institutes, and core facilities alike
How does NG-Assay-on-Request™ simplify NGS workflows?
NG-Assay-on-Request™ uses our Single Reaction Library Prep and Reverse Complement PCR to combine amplification, indexing, and library creation in one closed-tube reaction. This eliminates multi-step workflows, reduces contamination risk, and delivers sequencing-ready libraries faster, without requiring RC-PCR expertise.
No RC-PCR expertise? No problem. We’ve designed it so your targets, species, and sample volume drive the process, not the other way around
Products
Product related questions
I require technical support?
Our support staff is ready to answer any technical questions, should you run into any problems or need help troubleshooting.
Contact Customer Services at info@nimagen.com to connect you with the right support staff.
How do I make changes to an order I’ve already placed?
As soon as you place your order, we work on the dispatch process as quickly as possible, if you decide you want to amend your order please contact us and we will try to accommodate where possible.
When will my order ship out?
Orders have a processing time of 1-2 business days. Once dispatched, an email will be sent containing the tracking number for the parcel.
Which shipping carrier is used?
Depending on the shipping destination, DHL or FedEx is used. If preferred we also have options for UPS and TNT.
Can I track the status of my order?
Once your order is dispatched, an email will be sent containing the tracking number for the parcel. Lost your tracking number? Contact customer service at info@nimagen.com.
What can I do if my order is going to the wrong address?
Please contact us immediately, so we can attempt to contact the courier to return the parcel back to us for a refund.
I would like to pay by Credit Card, is this possible?
Yes, you can follow the normal order process and indicate this in the “Extra information” field. We will be in touch to process the CC payment.
What currencies can I use?
By default payment and invoicing is in Euro (EUR). Upon request we can also process Great British Pound (GBP) and United States Dollar (USD).
How do I return an item?
If you’re returning item(s) to us, please contact us with your name and order number at info@nimagen.com where we will be able to advise you further on our international returns process.
Please make sure temperature sensitive items are stored correctly in the meantime.
Is this product compatible with all Data Collection Software versions?
Yes, the NimaPOP pouches with integrated RFID tag are compatible with all Data Collection Software versions on 3500 Series Genetic Analyzers, including Applied Biosystems 3500 Series Data Collection Software v4.0 designed for data collection in human identification (HID) applications.
What is the maximum Ct value for a positive sample to be able to sequence?
We tested the kit with samples of Ct32 or lower. For samples with Ct <25 we recommend to dilute the cDNA
What can I do to sequence through a hairpin or secondairy structure?
- Use the BrilliantDye dGTP kit for the sequencing reaction. For optimal results it can be mixed with the default BrilliantDye kits to increase read lenghts.
- Add DMSO to a final 5% (v/v) concentration to the sequencing reaction.
- Incubate the reaction at 96C for 10 minutes before cycle sequencing.
How can I troubleshoot an issue with Sanger Sequencing?
A control primer (M13) and template (pGEM) are provided with each BrilliantDye™ Terminator Cycle Sequencing Kit. These can be run following the BrilliantDye™ protocol and can help you determine whether failed reactions are caused by poor template quality or sequencing reaction failure.
What is the difference between the different versions of BrilliantDye™?
The BrilliantDye™ Terminator v1.1 Cycle Sequencing Kit is designed for specialty applications that require optimal basecalling adjacent to the primer.
The BrilliantDye™ Terminator v3.1. Cycle Sequencing Kit is the method of choice for longest reads.
For sequencing through G- and GT-rich templates, NimaGen offers the dGTP BrilliantDye™ Terminator v1.1 and v3.1 Cycle Sequencing Kits, optimized for challenging sequence templates.
I would like to have more information about your products?
We work hard to include as much information about our products in our product descriptions on the product page, but if there’s something more specific you’d like to know please contact us at info@nimagen.com.
What is Reverse Complement PCR (RC-PCR) Technology?
Reverse Complement PCR (RC-PCR) is our patented, IP-protected technology, exclusively licensed to NimaGen B.V. by Salisbury NHS Foundation Trust. It powers our One-tube, Single Reaction NGS Library Prep, integrating amplification, indexing, and adapter ligation in a single closed reaction, unlike traditional PCR methods. Production, distribution, or use of RC-PCR-based methods or kits without written consent is strictly prohibited.
Is the workflow really closed-tube?
Yes. Amplification, indexing and adapter ligation take place in a single, closed reaction, minimizing handling and contamination risk.
Which sequencing platforms are supported?
Libraries are compatible with all major sequencing platforms.
How much hands-on time is required?
Preparing sequencing-ready libraries requires approximately 40 minutes of hands-on time, including pooled cleanup.
How many samples can be multiplexed?
Depending on index availability, up to 384 or more samples can be multiplexed in a single sequencing run.
How sensitive is the method?
The workflow supports reliable library preparation from low DNA input, down to the pg range, with balanced amplification and high specificity.
Do I need specialist training?
No specialist training is required. The workflow is designed to be as straightforward as a standard PCR protocol.
Can libraries be pooled before cleanup?
Yes. Individual reactions can be pooled prior to cleanup, reducing hands-on time and consumable use.
Is automation possible?
Yes. The workflow can be implemented on a variety of liquid handling platforms.
What are custom NGS panels?
NG-Assay-on-Request™ provides fully customized NGS library prep kits for targeted sequencing, designed around your specific genomic targets rather than a generic catalogue selection. Built from scratch, or designed to extend the content of an existing panel. Free project consultation with our NGS experts is included, dedicated to a quick and practical turnaround.
Why might standard NGS panels not meet my specific research needs?
Most commercial panels are built for the broadest possible market, which means they are rarely optimized for any single research context.
Too narrow: Your variants of interest are absent from every available commercial panel, forcing you to accept incomplete coverage or invest months in an in-house design.
Too broad: You pay for sequencing depth across targets that add no value to your study, driving up costs without improving the quality of your results.
Time-consuming: Designing a custom panel in-house requires bioinformatics capacity for in silico probe design, wet-lab time for iterative validation, and expertise most diagnostic or research labs cannot spare, with development timelines that routinely stretch to several months.
Unreliable: Self-designed panels introduce risk at every open-tube step, from probe design failures and uneven GC content to indexing errors and cross-contamination during library preparation.
The kit should fit your science, not the other way around, and that is precisely the problem NG-Assay-on-Request™ was built to solve.
What results can I expect from NG-Assay-on-Request™?
NG-Assay-on-Request™ delivers high on-target performance (≥70% reads), uniform coverage (≥95% within 3-fold of median), and reproducible results, all in a single reaction. Expect faster turnaround, fewer errors, and full traceability for your custom targets.
What applications and biological domains can NG-Assay-on-Request™ support?
NG-Assay-on-Request™ supports a wide range of applications across human, animal, plant, and microbiology domains, including hereditary diseases, cancer, forensic identification, breeding programs, QA/QC, pathogen detection, crop improvement, strain typing, and resistance analysis. If your target database exists, we can design a validated panel around it
How does the NG-Assay-on-Request™ process work?
The NG-Assay-on-Request™ process consists of four steps:
1. Submit your species, genome, targets, platform, and sample volume.
2. Design by our R&D team, ensuring specificity and balance.
3. Validate through wet-lab confirmation of on-target performance and coverage uniformity.
4. Deliver your ready-to-use kit, assembled and shipped.
This tailored process meets the needs of clinical labs, research institutes, and core facilities alike
How does NG-Assay-on-Request™ simplify NGS workflows?
NG-Assay-on-Request™ uses our Single Reaction Library Prep and Reverse Complement PCR to combine amplification, indexing, and library creation in one closed-tube reaction. This eliminates multi-step workflows, reduces contamination risk, and delivers sequencing-ready libraries faster, without requiring RC-PCR expertise.
No RC-PCR expertise? No problem. We’ve designed it so your targets, species, and sample volume drive the process, not the other way around
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