You did all the hard work extracting DNA and prepare it for sequencing. Your PCR works perfectly. Your library prep looks solid. But then you get your sequencing output and those short unwanted fragments are everywhere in your data.
You know the problem
AmpliClean™ LF solves this
Building on NimaGen's proven magnetic bead technology as a trusted drop-in alternative to AMPure® XP, AmpliClean™ LF is specifically optimized for long DNA fragments with a size-selection range of 1 - 6 kb. While standard cleanup beads struggle with efficient size selection in this range, AmpliClean™ LF is engineered from the ground up to bind long fragments efficiently and remove short contaminants superior to competing beads.
AMPLICLEAN WORKS
Why AmpliClean™ LF outperforms standard beads
Superior fragment cleanup
AmpliClean™ LF removes unwanted short DNA fragments more efficiently than competing magnetic beads and our standard AmpliClean™ kit. The optimized bead formulation ensures reliable size selection in any NGS workflow, giving you cleaner data with less background noise.
Cost-effective performance
Delivers superior long-fragment cleanup at a competitive price point, making it an economical choice for both routine and high-throughput sequencing applications.
Customer-validated performance
Real-world data from customer applications shows a market improvement in sequencing output. In full-length 16S sequencing with Oxford Nanopore Technologies, the first customers showed complete removal of contaminating DNA fragments between 400bp - 1000bp.
THREE-STEP PROCESS
The workflow you know, now optimized
AmpliClean™ LF follows the same trusted three-step process you rely on:
- Bind – Long DNA fragments (1-6 kb) selectively bind to paramagnetic beads
- Wash – Salts, primers, dimers, nucleotides, and short contaminants are removed with ethanol
- Elute – Ultra-pure DNA is recovered, directly ready for downstream applications
No centrifugation. No vacuum filtration. Just reliable, high-quality DNA in three simple steps.
Automation-ready for any lab
Whether you run manual single-tube workflows or high-throughput sequencing facilities, AmpliClean™ LF adapts to your setup. Fully compatible with Alpaqua® magnet plates and liquid handlers, making it ideal for labs scaling up their NGS operations.
COMPARISON
AmpliClean™ LF vs. AmpliClean™ standard beads
Independent comparison shows AmpliClean™ LF delivers:
- Significantly improved removal of fragments <1 kb
- Improved sequencing output, thereby reducing cost
- Consistent performance across different fragment size ranges
Download our comparison leaflet (PDF) to see detailed performance data comparing AmpliClean™ LF with standard AmpliClean™ and competitor beads.
Ideal applications
- Long-read sequencing workflows (Oxford Nanopore, PacBio)
- Full-length 16S/18S amplicon sequencing
- Library preparation where long fragment size accuracy is critical
- Any NGS workflow requiring clean removal of short background DNA
Product availability
AmpliClean™ LF is available in three formats to match your throughput needs:
- 5 mL – for small-scale applications and pilot studies
- 50 mL – for medium-throughput laboratories
- 500 mL – for high-throughput sequencing facilities
Order directly in our webshop or request a test sample below.
Request your AmpliClean™ LF test sample
Want to see the difference for yourself? Request a test sample of AmpliClean™ LF and experience superior long-fragment cleanup in your own workflow.
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