IDseek® OmniSTR™ Validated: A Breakthrough in Forensic DNA

We are excited to share the publication by Kristiaan van der Gaag of the Netherlands Forensic Institute in Forensic Science International: Genetics:

"Validation of the IDseek® OmniSTR™ Global Autosomal STR Profiling Kit"

This study highlights Reverse Complement PCR (RC-PCR) as an improved tool for routine massively parallel sequencing (MPS) of short tandem repeats (STRs). Read the full article on Elsevier: https://doi.org/10.1016/j.fsigen.2024.103174

Highlights:

• OmniSTR™ performance for minimal DNA amounts exceeds current MPS and CE methods;
• SE33 sequence variation results in many more variants compared to length variation;
• OmniSTR™ tolerance for inhibitors Humic and Tannic acid is comparable to PPF6C but improved for EtOH;
• Automated analysis of references achieved 99.94 % accuracy for 96.7 % called markers;
• Minor alleles down to a level of 1.5 % of the major can be called in mixtures for OmniSTR™

With the introduction of the novel and innovative Reverse Complement PCR (RC-PCR) technology, NimaGen is setting a new standard in forensic DNA analysis with their IDseek® Forensic MPS Kit Portfolio. This breakthrough allows the forensic community to safely and simply transition from classic Capillary Electrophoresis (CE) to Massively Parallel Sequencing (MPS) without compromise

Key Benefits:

• Closed-tube workflow:
  A single reaction combining indexing and target amplification reduces labor time, minimizes pipetting errors, and prevents sample swapping or contamination;
• Enhanced specificity and sensitivity:
  RC-PCR’s reaction kinetics improve recovery of more complete profiles from challenging samples;
• Cost-efficient sequencing:
  High on-target read percentages reduce off-target and primer-dimer reads, optimizing flow cell capacity and lowering sequencing costs.

Learn more: IDseek® OmniSTR™ Global Autosomal STR Profiling Kit

Looking for more information? Our team is here to help. Reach out today to explore how RC-PCR technology can benefit your lab.

• Email: p.vanoers@nimagen.com
• Phone: +31 24 820 02 40