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EasySeq™ BRCA1/2 + CHEK2 HS is purpose-designed to aid in the screening of congenital predisposition to breast cancer by detecting sequence variants.
The RC-PCR powered NGS library preparation is aimed at the re-sequencing of the human BRCA1 and BRCA2 gene coding exons, including a minimum of 20 bases upstream and downstream of each exon. The assay also includes the CHEK2 1100del C mutation (NM_001005735) hotspot.
For Research Use Only.
The multiplex RC-PCR generates amplicons that can be sequenced on Illumina® platforms iSeq-100, MiniSeq, MiSeq, NextSeq 500/550 and NextSeq 1000/2000 and more. Depending on the platform, up to 768 samples can be multiplexed.
Please note: EasySeq™ BRCA1/2 + CHEK2 HS Sequencing Kit is designed to sequence the BRCA1, BRCA2 and CHEK2 HS regions in a multiplex reaction using two different probe panels. This requires two Unique Dual Index Plates for associated use.
The EasySeq™ BRCA1/2 + CHEK2 HS Sequencing Kit allows direct amplification of DNA extracted from peripheral blood. Specimens should have an absorbance ratio (260/280) of ~1.8, regardless of the extraction method used. The kit is tested and validated for an input DNA amount of 20 ng.