IDseek® mYSTR™ MPS Library Normalization Kit 96 rxn

mYSTR™ MPS Library Normalization Kit (96 rxn) is a qPCR assay for relative read-depth normalization of mYSTR™ libraries, enabling laboratories to balance sequencing output across samples prior to massively parallel sequencing.    Achieving consistent read depth in forensic MPS can be challenging, especially when working with casework samples or degraded or ancient DNA. Variations in sample quantity and quality often lead to uneven read depths: some samples fall below analysis thresholds, while others consume excessive sequencing capacity and increase overall costs. To address this challenge, sample-to-sample normalization is essential.   The mYSTR™ MPS Library Normalization Kit provides an efficient qPCR-based method for relative read depth normalization specifically designed for mYSTR™ libraries. Using nested targeted primers instead of conventional Illumina® adapter-based primers, the assay offers a robust and user-friendly workflow that delivers optimized sample-to-sample read depth normalization across a wide range of input qualities and quantities.    Kit Components  •     mYSTR™ qPCR Primer Set •     2x qPCR Master Mix    Benefits ✓ Balances sample-to-sample read depth ✓ Maximizes use of available sequencing capacity ✓ Accurate normalization across input quantities ✓ Target-specific nested primer design ✓ Automation-compatible workflow ✓ Simple and robust qPCR workflow