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EasySeq™ CFTR Sequencing Kit, 2 pools/sample, incl. PCR Master Mix, 96 rxn

SKU: RC-CFTR096
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Product Information

Targeted Resequencing of Cystic Fibrosis Associated CFTR Gene Mutations

Intended use

EasySeq™ CFTR Sequencing Kit, powered by patented Reverse Complement PCR (RC-PCR) technology, facilitates an accurate detection of published cystic fibrosis transmembrane regulator (CFTR) mutations in a targeted resequencing assay, that is purpose-designed for fast and efficient lab processing. 

 

EasySeq™ CFTR Sequencing Kit offers a one-tube single-reaction multiplex assay, comprising an optimized panel of 41 human CFTR gene coding exons, including a minimum of 20 bases upstream and downstream of each exon, plus 2 flanking intronic hotspot mutations (rs397508266 and rs75039782), providing a 100% gene coverage.

 

For Research Use Only. Not for use in diagnostic procedures.

 

Compatibility

The multiplex RC-PCR generates 43 amplicons that can be sequenced on Illumina® benchtop platforms iSeq, MiniSeq, MiSeq, NextSeq and production-scale sequencers HiSeq and NovaSeq. 

 

The EasySeq™ CFTR Sequencing Kit contains two multiplex PCR primers pools and PCR Master Mix. Universal Index Plates and AmpliClean™ magnetic beads are ordered separately to complete the workflow from input DNA to sequencing-ready NGS libraries.

 

Please note: EasySeq™ CFTR Sequencing Kit is designed to sequence the set of 43 exonic targets using a two probe pool reaction. This requires two Unique Dual Index Plates for associated use.

 

The EasySeq™ CFTR Sequencing Kit allows direct amplification of DNA isolated from human peripheral blood, saliva or buccal swab.

 

Specimens should have an absorbance ratio (260/280) of ~1.8, regardless of the extraction method used. The kit is tested and validated for an input DNA amount of 20 ng.

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Premium features

Cost-efficient Workflow - Confidence in Test Results - Choice in Sequencing Platform and Workload

One closed-tube, single reaction workflow with simultaneous indexing and target amplification reduce hands-on time, pipetting error and minimizes risk of sample contamination
Pre-spotted and breakable Unique Dual Index plates ensure accuracy, match your sample workload and ensure optimal usage, minimizing waste
Single tube sample pooling for library clean-up significantly reduces usage of required magnetic beads and consumables
Unique RC-PCR kinetics promote high-target specificity (100% gene coverage), sensitivity and coverage uniformity (optimized read depth balance, >95%) from low DNA input
Well-balanced read distributions and use of phase-shifted primers reduces PhiX spike-in, maximizing sequencing instrument flow cell capacity
Compatible with various Illumina® platforms and liquid handling systems for medium to high-throughput workflows