Product

BrilliantDye™ Terminator (v1.1) Cycle Sequencing Kit (25000 rxn)

SKU: BRD1-25K
Variants
BRD1-25K
€ 175.850,00
Variants
€ 175.850,00
Product Information

BrilliantDye™ Terminator (v1.1) Cycle Sequencing Kit

Format

BrilliantDye™ Terminator (v1.1) Cycle Sequencing Kit provides the reagents (BrilliantDye Terminator, 5x sequencing buffer, pGEM control, M13 control primer) required for Sanger Sequencing reactions, in a ready for use pre-mixed format. It is optimized to provide a robust and highly flexible chemistry, designed for all kinds of DNA sequencing applications, including de novo and resequencing.

 

BrilliantDye™ Terminator (v1.1) Cycle Sequencing Kit is conveniently offered in low to medium to high-throughput packaging, ranging from 24, 100, 1,000, and 5,000 to 25,000 reactions respectively.

Compatibility

BrilliantDye™ Terminator Cycle Sequencing kits and associated buffers are widely adopted as proven, high-quality reagents for laboratories using 310, 3100, 3130, 3500, 3730 Series Genetic Analyzers and SeqStudio™.

 

BrilliantDye™ is developed for performing fluorescence-based cycle sequencing reactions on single-stranded or double-stranded DNA templates, including PCR fragments and plasmids. BrilliantDye™ generates data with uniform peak heights and optimized signal balance to produce long, high-quality reads, as well as highly accurate base assignments.

 

BrilliantDye™ Terminator v1.1 Cycle Sequencing Kits are a drop-in replacement for BigDye® Terminator v1.1 Cycle Sequencing Kits without having to change protocol, volume, or setting. The products can be used with other products in the Sanger sequencing workflow (e.g. NimaPOP™ or POP™ polymers and buffers) on Applied Biosystems® 3130, 3500, 3730 and SeqStudio™ Flex Genetic Analyzers.

 

BrilliantDye and NimaPOP are trademarks of NimaGen, B.V. SeqStudio and POP are a trademark of Thermo Fisher Scientific, Inc.

Premium features
FAQ
Downloads
Premium features

BrilliantDye™ is developed for performing fluorescence-based cycle sequencing reactions on single-stranded or double-stranded DNA templates, including PCR fragments and plasmids. BrilliantDye™ generates data with uniform peak heights and optimized signal balance to produce long, high-quality reads, as well as highly accurate base assignments.

 

BrilliantDye™ Terminator v1.1 Cycle Sequencing Kits are a drop-in replacement for BigDye® Terminator v1.1 Cycle Sequencing Kits without having to change protocol, volume, or setting. The products can be used with other products in the Sanger sequencing workflow (e.g. NimaPOP™ or POP™ polymers and buffers) on Applied Biosystems® 3130, 3500, 3730 and SeqStudio™ Flex Genetic Analyzers

Based on the proven Sanger Chain Termination method
No need for changes in run protocol, setting or calibration
Available in chemistry versions v1.1, v3.1 and dGTP (v1.1 and v3.1)
Compatible with all Genetic Analyzers
FAQ

How can we help to find the right answer for your question? If your question is not listed below, please use the contact form.

How can I troubleshoot an issue with Sanger Sequencing?

A control primer (M13) and template (pGEM) are provided with each BrilliantDye™ Terminator Cycle Sequencing Kit. These can be run following the BrilliantDye™ protocol and can help you determine whether failed reactions are caused by poor template quality or sequencing reaction failure.

What is the difference between the different versions of BrilliantDye™?

The BrilliantDye™ Terminator v1.1 Cycle Sequencing Kit is designed for specialty applications that require optimal basecalling adjacent to the primer.

The BrilliantDye™ Terminator v3.1. Cycle Sequencing Kit is the method of choice for longest reads.

For sequencing through G- and GT-rich templates, NimaGen offers the dGTP BrilliantDye™ Terminator v1.1 and v3.1 Cycle Sequencing Kits, optimized for challenging sequence templates.

Product news

Latest news

News | 08.01.24

NimaGen's 2023 Highlights ReCAPPed!

NimaGen had a great 2023 and were looking forward to an even better 2024!    NimaGen’s 2023 ReCAPPed™ (Yes, this a pun on our product name):  Our New Building and Facilities:  We kicked off 2023 by moving into our new building with state-of-the-art laboratory and production spaces, as well as comfortable office and meeting spaces! Our NimaGen Grand Opening party in May was an exciting event celebrated by all to commemorate such a momentous occasion; it was also attended by our local major of Nijmegen!            Our New Reverse Complement PCR Sequencing Kits:  From there, we spent the year developing several innovative and exciting new Reverse-Complement PCR sequencing kits for our application areas in Forensics, Microbiology and Infectious Disease and Human Genetics!      Distributors, Partnerships and Collaborations In 2023, NimaGen gained 15 partnerships around the world that help support our expanding product portfolio! From the far reaches of Barcode Biosciences in India to our neighboring country France supported by Dominique Dutscher SAS, as well as strategic collaborations with partnerships in North American with NicheVision for our Forensics portfolio, we desire to help our customers in every cornerstone of the world. Together, we will continue creating shared value!    Customer Experience Survey Rating At the end of 2023, active NimaGen customers received a customer experience survey with an average score of >8/10!. NimaGen responsiveness, solutions focus, personal interaction and product value were highly rated by both customers and distributors. We are grateful for the feedback we received and will continue to strive to continue improve our customer service, quality products and continued innovation that supports  research globally!    Continued ISO 90001 and 13485 Certification  NimaGen proudly continues to maintain its ISO 90001 and ISO 13485 certification. ISO 90001 is a globally recognized standard for quality management that ensures high-level customer service, ongoing optimization and continued process improvement. ISO 13485 is an international standard that specifies strict requirements for quality and competence in a medical device organization, including biotechnology companies, thus ensuring that we are providing high-quality reagents and products.  Congress and Event Attendance:  NimaGen attended several events around the world in 2023! Some conferences were local to the Netherlands such as Dutch Society of Medical Microbiology and the Royal Dutch Society of Microbiology (KNVM NVMM)  or our very own NimaGen Distributor Meeting here in Nijmegen! Other conferences were  within Europe, such as European Society of Clinical Microbiology and Infectious Diseases (ECCMID) in Denmark and the European Society of Human Genetics (ESHG) in Scotland. We also attended many international ones such as International Association of Forensic Sciences (IAFS) in Australia and the International Symposium on Human Identification (ISHI) for Forensics and the American Society for Human Genetics (ASHG) in the U.S.A!  Local: KNVM NVMM 2023 – Arnhem, The Netherlands    NimaGen Distributor Meeting 2023 - Creating Shared Value with our Partners  Europe: ECCMID 2023 – Copenhagen, Denmark   ESHG 2023 – Edinburgh, Scotland    International: IAFS 2023 – Sydney, Australia         ISHI 2023 – Denver, CO USA ASHG 2023 – Washington, D.C. USA             
News | 12.12.23

PT. Indolab Utama (Indonesia): Welcome to the NimaGen Distributor Network!

NimaGen is happy to announce the collaboration with @PT. Indolab Utama. Indolab Utama focusses on Research Institutes, Food and Beverage Industry, Pharmaceutical Industry, Hospital and Universities in the Indonesian market. They have supported several NimaGen users for some time already and we have now strengthened the partnership with an official distributorship. We look forward to working with Indolab Utama to support the customers in Indonesia better. 
News | 07.12.23

Dutscher (France): Welcome to the NimaGen Distributor Network!

NimaGen is happy to announce a new partnership with Dominique Dutscher SAS. Dutscher will be representing the NimaGen brands in France and support our customers in this region. The company was founded in 1982 and is now one of the largest distribution companies in Europe. They value service, quality, responsiveness and customization as well as a willingness to support customers in all their projects.  We look forward to working with Dutscher to increase the level of service for our French customers!