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BrilliantDye™ Terminator (v3.1) Cycle Sequencing Kit provides the reagents (BrilliantDye Terminator, 5x sequencing buffer, pGEM control, M13 control primer) required for Sanger Sequencing reactions, in a ready for use pre-mixed format.
BrilliantDye™ Terminator (v3.1) Cycle Sequencing Kit is conveniently offered in low to medium to high-throughput packaging, ranging from 24, 100, 1,000, and 5,000 to 25,000 reactions respectively.
BrilliantDye™ Terminator Cycle Sequencing kits and associated buffers are widely adopted as proven, high-quality reagents for laboratories using 310, 3100, 3130, 3500, 3730 Series Genetic Analyzers and SeqStudio™.
SeqStudio is a trademark of Applied Biosystems, LLC.
BrilliantDye™ Terminator (v3.1) Cycle Sequencing Kits offer robust, highly flexible chemistry, providing data with uniform peak heights and optimized signal balance, which results in longer, higher-quality sequencing reads.
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How can I troubleshoot an issue with Sanger Sequencing?
A control primer (M13) and template (pGEM) are provided with each BrilliantDye kit. These can be run following the BrilliantDye protocol and can help you determine whether failed reactions are caused by poor template quality or sequencing reaction failure.
What is the difference between the different versions of BrilliantDye?
The BrilliantDye Terminator v1.1 Cycle sequencing kit is designed for specialty applications that require optimal basecalling adjacent to the primer.
The BrilliantDye Terminator v3.1. Cycle sequencing kit is the method of choice for longest reads.
For sequencing through G- and GT-rich templates, NimaGen offers the dGTP BrilliantDye kits, optimized for challenging sequence templates.