BrilliantDye™ Terminator (v3.1) Cycle Sequencing Kit (5000 rxn)

SKU: BRD3-5000
Product Information

BrilliantDye™ Terminator (v3.1) Cycle Sequencing Kit


BrilliantDye™ Terminator (v3.1) Cycle Sequencing Kit provides the reagents (BrilliantDye Terminator, 5x sequencing buffer, pGEM control, M13 control primer) required for Sanger Sequencing reactions, in a ready for use pre-mixed format.


BrilliantDye™ Terminator (v3.1) Cycle Sequencing Kit is conveniently offered in low to medium to high-throughput packaging, ranging from 24, 100, 1,000, and 5,000 to 25,000 reactions respectively.


BrilliantDye™ Terminator Cycle Sequencing kits and associated buffers are widely adopted as proven, high-quality reagents for laboratories using 310, 3100, 3130, 3500, 3730 Series Genetic Analyzers and SeqStudio™.


SeqStudio is a trademark of Applied Biosystems, LLC.

Premium features
Premium features

BrilliantDye™ Terminator (v3.1) Cycle Sequencing Kits offer robust, highly flexible chemistry, providing data with uniform peak heights and optimized signal balance, which results in longer, higher-quality sequencing reads.

Based on the proven Sanger Chain Termination method
No need for changes in protocol, setting or calibration
Available in chemistry versions v1.1, v3.1 and dGTP (v1.1 and v3.1)
Compatible with all Genetic Analyzers

How can we help to find the right answer for your question. If your question is not listed below, please use the contact form.

How can I troubleshoot an issue with Sanger Sequencing?

A control primer (M13) and template (pGEM) are provided with each BrilliantDye kit. These can be run following the BrilliantDye protocol and can help you determine whether failed reactions are caused by poor template quality or sequencing reaction failure.

What is the difference between the different versions of BrilliantDye?

The BrilliantDye Terminator v1.1 Cycle sequencing kit is designed for specialty applications that require optimal basecalling adjacent to the primer.

The BrilliantDye Terminator v3.1. Cycle sequencing kit is the method of choice for longest reads.

For sequencing through G- and GT-rich templates, NimaGen offers the dGTP BrilliantDye kits, optimized for challenging sequence templates.

Product news

Latest news

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LifeGene (Israel): Welcome to the NimaGen Distributor Network!

We are happy to share that we have set up a new collaboration with LifeGene Ltd. As of March 1st, 2023, LifeGene will exclusively distribute the NimaGen portfolio in Israel. This Israeli company, based in Modi’in, has been a longstanding partner of NimaGen. Now, we are strengthening the ties by setting up an official distribution agreement. LifeGene serves customers in biological, chemical, forensic as well as diagnostic fields. We look forward to supporting our customers in Israel with this collaboration.   
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