Product

dGTP BrilliantDye™ Terminator (v3.1) Cycle Sequencing Kit (1000 rxn)

SKU: BRDG3-1000
Variants
BRDG3-1000
Variants
Product Information

dGTP BrilliantDye™ Terminator (v3.1) Cycle Sequencing Kit for Sequencing G- and GT-rich Templates

Format

dGTP BrilliantDye™ Cycle Sequencing Kits (v1.1 and v3.1) are designed and optimized for challenging sequence templates, by replacing the dITP with dGTP.

 

dGTP BrilliantDye™ Terminator (v3.1) Cycle Sequencing Kit provides the reagents (BrilliantDye Terminator, 5x sequencing buffer, DNA polymerase, pGEM control, M13 control primer) required for Sanger Sequencing of G- en GT-rich sequence templates, in a ready for use pre-mixed format.

 

dGTP BrilliantDye™ Terminator (v3.1) Cycle Sequencing Kit is conveniently offered in low to medium-throughput packaging, for 100 and 1,000 reactions respectively.

Compatibility

dGTP BrilliantDye™ Terminator (v3.1) Cycle Sequencing Kits are compatible with 310, 3100, 3130, 3500, 3730 Series Genetic Analyzers, and SeqStudio™.


dGTP BrilliantDye™ Terminator (v3.1) can be mixed with BrilliantDye™ (v3.1) for optimal results.

 

SeqStudio is a trademark of Applied Biosystems, LLC.

Premium features
FAQ
Downloads
Premium features

dGTP BrilliantDye™ Terminator (v3.1) Cycle Sequencing Kits offer robust, highly flexible chemistry, with uniform peak heights and optimized saignal balance. Replacement of dITP with dGTP enables to extend through those difficult-to-sequence regions with confidence, avoiding early signal loss in samples.

dGTP BrilliantDye™ Terminator v1.1 Cycle Sequencing Kit is designed for specialty applications that require optimal basecalling adjacent to the primer.

 

dGTP BrilliantDye™ Terminator v3.1 Cycle Sequencing Kit is the method of choice for longest reads.

Based on the proven Sanger Chain Termination method
No need for changes in run protocol, setting or calibration
Available in chemistry versions dGTP v1.1 and dGTP v3.1
Compatible with all Genetic Analyzers
FAQ

How can we help to find the right answer for your question. If your question is not listed below, please use the contact form.

What can I do to sequence through a hairpin or secondairy structure?

- Use the BrilliantDye dGTP kit for the sequencing reaction. For optimal results it can be mixed with the default BrilliantDye kits to increase read lenghts.

- Add DMSO to a final 5% (v/v) concentration to the sequencing reaction.

- Incubate the reaction at 96C for 10 minutes before cycle sequencing.

How can I troubleshoot an issue with Sanger Sequencing?

A control primer (M13) and template (pGEM) are provided with each BrilliantDye™ Terminator Cycle Sequencing Kit. These can be run following the BrilliantDye™ protocol and can help you determine whether failed reactions are caused by poor template quality or sequencing reaction failure.

What is the difference between the different versions of BrilliantDye™?

The BrilliantDye™ Terminator v1.1 Cycle Sequencing Kit is designed for specialty applications that require optimal basecalling adjacent to the primer.

The BrilliantDye™ Terminator v3.1. Cycle Sequencing Kit is the method of choice for longest reads.

For sequencing through G- and GT-rich templates, NimaGen offers the dGTP BrilliantDye™ Terminator v1.1 and v3.1 Cycle Sequencing Kits, optimized for challenging sequence templates.

Product news

Latest news

News | 13.09.23

NimaGen x NicheVision Partnership Announcement 

NimaGen and NicheVision are partnering to deliver a complete wet-to-dry lab solution for forensic DNA analysis!     This partnership aims to provide forensic DNA laboratories with a combined cutting-edge DNA sequencing and data analysis workflow for human identification, that helps DNA analysts resolve complex DNA mixtures through a fully continuous approach.   The partnership is a distribution and development agreement that grants NicheVision exclusive rights to distribute NimaGen products for forensic DNA analysis in North, Central and South America. The companies will collaborate to commercialize NimaGen’s Reverse Complement PCR powered IDseek® Massively Parallel Sequencing (MPS) workflow in combination with NicheVision MixtureAce™ expert forensic DNA analysis software; as well as NimaGen brand products (NimaPOP™, NimaPrime™) for Genetic Analyzer based Fragment Analysis.  Come see us at ISHI 2023 on Sept 18-21st in Denver, CO! NimaGen will be at Booth #313 and NicheVision will be at Booth #312. Come learn all about the next revolution in Forensic MPS!
News | 16.08.23

Triolab AS (Norway): Welcome to the NimaGen Distributor Network!

Creating Shared Value Recently, NimaGen has initiated a new collaboration with Triolab AS in Norway. We look forward to strengthening our service to customers in this region together with Triolab as our distributor. Triolab is based in Kjeller, just north of Oslo, and will exclusively represent our Sanger-CE and NGS Library Preparation portfolio. They have a strong scientific team that will be able to support researchers with their knowledge and expertise.    Contact information:  Triolab AS Jogstadveien 21 2007 KJELLER Norway   Phone: 0047 63835750 Email: info@triolab.no Website: https://www.triolab.no/
News | 13.07.23

Radboud University & NimaGen: Genetic technique reveals pathogens much more accurately

''‘For example, in the Netherlands we monitor coronavirus in hospitals and wastewater, but we can easily expand that to other pathogens’, Coolen says. ‘In addition, we already apply this technique weekly in clinical diagnostics to detect so-called non-tuberculosis mycobacteria, a growing medical problem.''   ''Especially when looking for tiny amounts of a pathogen's DNA in a sea of contamination, such as in wastewater. Jordy Coolen, a PhD student at Radboud university medical center, came up with several solutions to implement sequencing in routine care. His work also included a collaboration with the company NimaGen. With relatively simple steps and lower costs, he was able to very sensitively analyze the genome of microorganisms.''   Link to Radboud University collaboration article: https://www.radboudumc.nl/en/news-items/2023/genetic-technique-reveals-pathogens-much-more-accurately
News | 03.07.23

IVD Bulgaria Ltd (Bulgaria ): Welcome to the NimaGen Distributor Network!

We are happy to announce that we strengthened the collaboration with IVD Bulgaria. Our companies have been working together for several years and we have now solidified this with a Distributor Agreement for Bulgaria. Based in Sofia, Bulgaria, the company focusses on distribution of in-vitro laboratory reagents and consumables, tests and equipment used in human and veterinary diagnostics. We are happy to work together in supporting customers in Bulgaria!