Conventional Amplicon based methods for NGS target enrichment involve two PCR steps – one to amplify the regions of interest followed by a second PCR reaction to add adapter sequences for NGS. While these methods are well established there is always a high risk of cross contamination or carry-over from first to second rounds of amplification.
RC-PCR is IP protected.
US Patent / WO Patent / EP Patent
Exclusive license granted to NimaGen B.V., Nijmegen, The Netherlands
by SALISBURY NHS FOUNDATION TRUST (SALISBURY, WILTSHIRE, GB)
It is strictly prohibited to use, produce, distribute, import, or sell RC-PCR based methods or kits without the patent owner's written consent.
How it Works
Just one Pre-PCR reaction setup, quick, easy and highly suited for automation
Product generation is more target specific
Traditional PCR reactions have high concentrations of primer at the beginning of the reaction when the template availability is low.
In a RC-PCR the target specific primer is synthesized during the reaction and consequently more aligned with the target availability reducing the chance for dimerization and off target binding.
Predesigned and custom panels available targeting up to 100 amplicons in a single closed tube reaction.
- Human Sample tracking and ID kit for WGS/WES QC
- Forensic Identification Kits
- Crop Genotyping assays (GreenoType)
- GMO detection kit
- Meat detection kit (including speciate)
- Pharmacogenetics (PGX)
- Circulating Tumour DNA kit
- Detection of aneuploidies
EasySeq NGS RC-PCR products
EasySeq™ RC-PCR SARS CoV-2 (novel coronavirus) Whole Genome Sequencing kit (96 reactions).
PCR 2x Hotstart HiFi Mastermix, compatible with RC-PCR assays, 2.2 mL
Index Primer Plate U01 96 Unique Dual Indexes
Index Primer Plate U02 96 Unique Dual Indexes (#097-192)
Index Primer Plates U01 & U02
Two PCR plates, pre aliquoted with 4 uL Unique Dual Index Primers. Set U01
Two PCR plates, pre aliquoted with 4 uL Unique Dual Index Primers. Set U02
Two PCR plates, pre aliquoted with 4 uL Unique Dual Index Primers. Set U03
Two PCR plates, pre aliquoted with 4 uL Unique Dual Index Primers. Set U05
Two PCR plates, pre aliquoted with 4 uL Unique Dual Index Primers. Set U06
Human Identification and Sample tracking kit
NimaGen offers complete solutions for your custom NGS kits.
Please provide us with some info regarding your needs, and we will contact you as soon as possible.