Conventional Amplicon based methods for NGS target enrichment involve two PCR steps – one to amplify the regions of interest followed by a second PCR reaction to add adapter sequences for NGS. While these methods are well established there is always a high risk of cross contamination or carry-over from first to second rounds of amplification.
EasySeq™ NGS RC-PCR (Reverse Complement PCR) from NimaGen is the ideal solution to these concerns with a single closed tube PCR step for both amplification and indexing.
Just one Pre-PCR reaction setup, quick, easy and highly suited for automation
Traditional PCR reactions have high concentrations of primer at the beginning of the reaction when the template availability is low.
In an RC-PCR the target specific primer is synthesised during the reaction and consequently more aligned with the target availability reducing the chance for dimerization and off target binding.
Predesigned and custom panels available targeting up to 100 amplicons in a single closed tube reaction.
NimaGen offers complete solutions for your custom NGS kits.
Please provide us with some info regarding your needs, and we will contact you as soon as possible.